About: Ribosomal RNA depletion or exclusion has negligible effect on the detection of viruses in a pan viral microarray

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About: Ribosomal RNA depletion or exclusion has negligible effect on the detection of viruses in a pan viral microarray Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Ribosomal RNA depletion or exclusion has negligible effect on the detection of viruses in a pan viral microarray
Creator	Dastjerdi, Akbar Steinbach, Falko Blake, Damer Nunez-Garcia, Javier Mcgowan, Sarah Rocca, Anna
source	Elsevier; Medline; PMC
abstract	Pan viral DNA microarrays, which can detect known, novel and multiple viral infections, are major laboratory assets contributing to the control of infectious diseases. The large quantity of ribosomal RNA (rRNA) found in tissue samples is thought to be a major factor contributing to the comparatively lower sensitivity of detecting RNA viruses, as a sequence-independent PCR is used to amplify unknown samples for microarray analysis. This study aimed to determine whether depletion or exclusion of rRNA can improve microarray detection and simplify its analysis. Therefore, two different rRNA depletion and exclusion protocols, RiboMinus™ technology and non-rRNA binding hexanucleotides, were applied to the microarray sample processing and the outcome was compared with those of the sequence-independent amplification protocol. This study concludes that the two procedures, described to deplete or exclude rRNA, have negligible effect on the microarrays detection and analysis and might only in combination with further techniques result in a significant enhancement of sensitivity. Currently, existing protocols of random amplification and background adjustment are pertinent for the purpose of sample processing for microarray analysis.
has issue date	2014-07-14(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2014.07.012
bibo:pmid	25034125
has license	no-cc
sha1sum (hex)	b6d49512ce3a1a6d71dc5e5c45a8bd9e76662b5b
schema:url	https://doi.org/10.1016/j.jviromet.2014.07.012
resource representing a document's title	Ribosomal RNA depletion or exclusion has negligible effect on the detection of viruses in a pan viral microarray
has PubMed Central identifier	PMC7119560
has PubMed identifier	25034125
schema:publication	J Virol Methods
resource representing a document's body	covid:b6d49512ce3a1a6d71dc5e5c45a8bd9e76662b5b#body_text
is schema:about of	named entity 'VIRUSES' named entity 'microarray' named entity 'DNA microarrays' named entity 'technology' named entity 'infectious diseases' named entity 'protocols' named entity 'PAN' named entity 'DNA MICROARRAYS' named entity 'MAJOR' named entity 'AMPLIFICATION PROTOCOL' named entity 'OUTCOME' named entity 'TISSUE' named entity 'EXCLUSION OF' named entity 'NON-' named entity 'UNKNOWN' named entity 'LABORATORY' named entity 'SIMPLIFY' named entity 'PAN' named entity 'INFECTIOUS DISEASES' named entity 'USED' named entity 'CONCLUDES' named entity 'THOUGHT' named entity 'FOUND' named entity 'MICROARRAYS' named entity 'RRNA DEPLETION' named entity 'SIGNIFICANT' named entity 'KNOWN' named entity 'ADJUSTMENT' named entity 'SAMPLE PROCESSING' named entity 'ENHANCEMENT' named entity 'MICROARRAY ANALYSIS' named entity 'DEPLETION' named entity 'RRNA' named entity 'SEQUENCE' named entity 'DEPLETION' named entity 'EXCLUSION' named entity 'MICROARRAY' named entity 'NOVEL' named entity 'ITS' named entity 'EXCLUDE' named entity 'CONTRIBUTING' named entity 'RANDOM' named entity 'A MAJOR' named entity 'AMPLIFICATION' named entity 'STUDY' named entity 'LOWER' named entity 'PURPOSE' named entity 'HAVE' named entity 'AMPLIFY' named entity 'PCR' named entity 'SAMPLES' named entity 'BACKGROUND' named entity 'DETECTING' named entity 'EXCLUSION' named entity 'EFFECT' named entity 'NEGLIGIBLE' named entity 'MULTIPLE' named entity 'INDEPENDENT' named entity 'RNA VIRUSES' named entity 'VIRAL INFECTIONS' named entity 'MICROARRAY' named entity 'RIBOSOMAL RNA' named entity 'CONTROL' named entity 'DIFFERENT' named entity 'PROTOCOLS' named entity 'RRNA BINDING' named entity 'COMBINATION' named entity 'VIRAL' named entity 'DETECTION' named entity 'DESCRIBED' named entity 'COMPARED' named entity 'DETECTION' named entity 'NEGLIGIBLE' named entity 'QUANTITY' named entity 'TECHNOLOGY' named entity 'DETECT'

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