About: The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes

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About: The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes
Creator	Chen, Zhiwei Zhang, Linqi Liu, Li Tien, Po Fang, Qing Wang, Haibo Yu, Wenbo Zhou, Jingying Zhu, Weijun Zhuang, Ke
Source	Elsevier; Medline; PMC
abstract	To generate a safe vaccinia Tian Tan (VTT)-based vaccine vector, it is necessary to develop a method to attenuate the virus. A modified VTT (MVTT(2-GFP)) was constructed by replacing the viral M1L-K2L genes with a GFP gene. In comparison to the parental VTT, MVTT(2-GFP) lost its replication capacity in rabbit RK13 and human HeLa cell lines. The life cycle of viral replication was blocked at different stages in these two cell lines as determined by electron microscope examination. MVTT(2-GFP) was less virulent than VTT for 100-fold by measuring mouse body weight loss after intranasal viral inoculation and for 340-fold by determining the intracranial LD(50) value in mice. The foreign GFP gene was stable genetically after 10 rounds of passage in Vero cells. Importantly, MVTT(2-GFP) elicited both humoral and cell-mediated immune responses to the GFP gene in mice. With two intramuscular inoculations of 10(5) PFU virus, the anti-GFP antibody reciprocal endpoint titer reached over 700 as determined by an ELISA. The number of IFN-γ secreting T cells reached over 350 SFU per million splenocytes against a CD8+ T cell-specific epitope of GFP. Collectively, the removal of the M1L-K2L genes is a useful method to generate an attenuated vaccinia Tian Tan vaccine vector.
has issue date	2007-04-24(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2007.03.012
bibo:pmid	17459491
has license	no-cc
sha1sum (hex)	b715b120180b24a6efb23ca38fb3bdea33ac3e40
schema:url	https://doi.org/10.1016/j.jviromet.2007.03.012
resource representing a document's title	The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes
has PubMed Central identifier	PMC7112875
has PubMed identifier	17459491
schema:publication	J Virol Methods
resource representing a document's body	covid:b715b120180b24a6efb23ca38fb3bdea33ac3e40#body_text
is schema:about of	named entity 'GENES' named entity 'vaccinia' named entity 'reached' named entity 'titer' named entity 'intranasal' named entity 'mouse' named entity 'Tan' named entity 'STRAIN' named entity 'ATTENUATION' named entity 'GENE' named entity 'FOLD' named entity 'DETERMINED BY' named entity 'VALUE' named entity 'VIRAL' named entity 'MEDIATED' named entity 'BASED' named entity 'MICE' named entity 'THESE' named entity 'SPLENOCYTES' named entity 'PFU' named entity 'FOREIGN' named entity 'EXAMINATION' named entity 'INOCULATION' named entity 'METHOD' named entity 'HELA CELL' covid:arg/b715b120180b24a6efb23ca38fb3bdea33ac3e40 named entity 'REMOVAL' named entity 'CELL' named entity 'ENDPOINT' named entity 'LIFE CYCLE' named entity 'M1L' named entity 'STAGES' named entity 'GFP' named entity 'CAPACITY' named entity 'STABLE' named entity '90%' named entity 'BODY WEIGHT LOSS' named entity 'LOST' named entity 'TITER' named entity 'HUMAN' named entity 'ANTIBODY' named entity '700' named entity 'SPECIFIC' named entity 'IS A' named entity 'IMMUNE RESPONSES' named entity 'VIRAL REPLICATION' named entity 'REMOVAL' named entity 'GENERATE' named entity 'GENES' named entity 'INTRANASAL' named entity 'VACCINE' named entity 'NECESSARY' named entity 'MOUSE' named entity 'IFN' named entity 'LESS VIRULENT' named entity 'ATTENUATE' named entity 'RECIPROCAL' named entity 'SECRETING' named entity 'T CELL' named entity 'CD8' named entity '350' named entity 'COMPARISON' named entity 'PARENTAL' named entity 'PASSAGE' named entity 'M1L' named entity 'INTRACRANIAL' named entity 'T CELLS' named entity 'ELICITED' named entity 'ELECTRON MICROSCOPE' named entity 'ELISA' named entity 'CONSTRUCTED' named entity '340'

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