About: Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodies

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodies
Creator	Liu, Y Fong, Carol Lingwood, Daniel Deantonio, Cecilia Gasparrini, Francesca Kjaer, Svend Maldonado, Paula Meffre, Eric Nair, Usha Ordovas-Montanes, Jose Pecetta, Simone Poignard, Pascal Arpin, C Banchereau, J Batista, Facundo Borley, Daryl Brière, F Burton, Dennis Coleman, Julia Déchanet, J Grouard, G Kazer, Samuel Kooten, C Merville, P Nandin, Irene Shalek, Alex Torreno-Pina, Juan
source	Medline; PMC
abstract	Vaccines remain the most effective tool to prevent infectious diseases. Here, we introduce an in vitro booster vaccination approach that relies on antigen-dependent activation of human memory B cells in culture. This stimulation induces antigen-specific B cell proliferation, differentiation of B cells into plasma cells, and robust antibody secretion after a few days of culture. We validated this strategy using cells from healthy donors to retrieve human antibodies against tetanus toxoid and influenza hemagglutinin (HA) from H1N1 and newly emergent subtypes such as H5N1 and H7N9. Anti-HA antibodies were cross-reactive against multiple subtypes, and some showed neutralizing activity. Although these antibodies may have arisen as a result of previous influenza infection, we also obtained gp120-reactive antibodies from non–HIV-infected donors, indicating that we can generate antibodies without prior antigenic exposure. Overall, our novel approach can be used to rapidly produce therapeutic antibodies and has the potential to assess the immunogenicity of candidate antigens, which could be exploited in future vaccine development.
has issue date	2017-08-07(xsd:dateTime)
bibo:doi	10.1084/jem.20170633
bibo:pmid	28739603
has license	cc-by
sha1sum (hex)	bbf36eb3250211e0c223d0c2a9c41abcaa32d11a
schema:url	https://doi.org/10.1084/jem.20170633
resource representing a document's title	Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodies
has PubMed Central identifier	PMC5551578
has PubMed identifier	28739603
schema:publication	J Exp Med
resource representing a document's body	covid:bbf36eb3250211e0c223d0c2a9c41abcaa32d11a#body_text
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