About: Avian influenza A/H5N1 neuraminidase expressed in yeast with a functional head domain

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About: Avian influenza A/H5N1 neuraminidase expressed in yeast with a functional head domain Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Avian influenza A/H5N1 neuraminidase expressed in yeast with a functional head domain
Creator	Boonyapakron, K Chitnumsub, P Eurwilaichitr, L Jongkaewwattana, A Leartsakulpanich, U Wanitchang, A Yongkiettrakul, S Yuthavong, Y
source	Elsevier; Medline; PMC
abstract	The study reports heterologous expression in Pichia pastoris of active neuraminidase derived from avian influenza virus A/Viet Nam/DT-036/2005(H5N1). A gene encoding the neuraminidase N1 head domain (residues 63–449) was fused directly in-frame with the Saccharomyces cerevisiae α-factor secretion signal in pPICZ(A vector. Recombinant N1 neuraminidase was expressed in P. pastoris as a 72 kDa secreted, soluble protein. Glycopeptidase F treatment generated a 45 kDa product, indicating that the secreted recombinant N1 neuraminidase is an N-linked glycoprotein. Kinetic studies and inhibition tests with oseltamivir carboxylate demonstrated that the recombinant N1 neuraminidase has similar K(m) and K(i) values to those of the viral N1 neuraminidase. This yeast-based heterologous expression system provided functionally active recombinant N1 neuraminidase that should be useful in anti-influenza drug screening, and also as a potential protein-based vaccine.
has issue date	2008-12-11(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2008.10.025
bibo:pmid	19038288
has license	no-cc
sha1sum (hex)	bdb8437f5a200b0e581313d4110acd3aa4e0398d
schema:url	https://doi.org/10.1016/j.jviromet.2008.10.025
resource representing a document's title	Avian influenza A/H5N1 neuraminidase expressed in yeast with a functional head domain
has PubMed Central identifier	PMC7112848
has PubMed identifier	19038288
schema:publication	J Virol Methods
resource representing a document's body	covid:bdb8437f5a200b0e581313d4110acd3aa4e0398d#body_text
is schema:about of	named entity 'anti-influenza' named entity 'kDa' named entity 'study' named entity 'avian influenza' named entity 'inhibition' named entity 'factor' named entity 'YEAST' named entity 'DIRECTLY' named entity 'DERIVED' named entity 'INFLUENZA VIRUS A' named entity 'PROVIDED' named entity 'VACCINE' named entity 'NAM' named entity 'VALUES' named entity 'ENCODING' named entity '036' named entity 'USEFUL' named entity 'A GENE' named entity 'BASED' named entity 'ANTI-INFLUENZA DRUG' named entity 'AVIAN INFLUENZA VIRUS' named entity 'FRAME' named entity 'HEAD' named entity 'HETEROLOGOUS EXPRESSION' named entity 'SOLUBLE PROTEIN' named entity 'FACTOR' covid:arg/bdb8437f5a200b0e581313d4110acd3aa4e0398d named entity 'GLYCOPEPTIDASE' named entity 'VECTOR' named entity '90%' named entity 'RECOMBINANT' named entity 'DEMONSTRATED' named entity 'OSELTAMIVIR CARBOXYLATE' named entity 'NEURAMINIDASE' named entity 'A 72' named entity 'TREATMENT' named entity 'KINETIC' named entity 'GENERATED' named entity 'TESTS' named entity 'REPORTS' named entity 'INDICATING' named entity 'ACTIVE' named entity 'SECRETED' named entity 'NEURAMINIDASE' named entity 'EXPRESSION SYSTEM' named entity 'PRODUCT' named entity 'SIMILAR' named entity 'EXPRESSED' named entity 'AVIAN INFLUENZA A' named entity 'HEAD' named entity 'DOMAIN' named entity 'SIGNAL' named entity 'YEAST' named entity 'STUDIES' named entity 'POTENTIAL' named entity '28A' named entity 'DOMAIN' named entity 'VIRAL' named entity 'INHIBITION' named entity 'PICHIA PASTORIS' named entity 'KDA' named entity 'FUNCTIONAL' named entity 'PROTEIN' named entity 'SACCHAROMYCES CEREVISIAE' named entity 'STUDY' named entity 'DRUG SCREENING' named entity 'SECRETION' named entity 'FUSED' named entity 'N-LINKED GLYCOPROTEIN' named entity 'EXPRESSED' named entity 'oseltamivir' named entity 'indicating' named entity 'active' named entity 'neuraminidase'

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