About: Rapid one-step construction of a Middle East Respiratory Syndrome (MERS-CoV) infectious clone system by homologous recombination

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About: Rapid one-step construction of a Middle East Respiratory Syndrome (MERS-CoV) infectious clone system by homologous recombination Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Rapid one-step construction of a Middle East Respiratory Syndrome (MERS-CoV) infectious clone system by homologous recombination
Creator	Leung, Anders Kobasa, Darwyn Theriault, Steven Cook, Bradley Court, Deborah Nikiforuk, Aidan
source	Elsevier; Medline; PMC
abstract	Abstract Background Viral Infectious clone systems serve as robust platforms to study viral gene or replicative function by reverse genetics, formulate vaccines and adapt a wild type-virus to an animal host. Since the development of the first viral infectious clone system for the poliovirus, novel strategies of viral genome construction have allowed for the assembly of viral genomes across the identified viral families. However, the molecular profiles of some viruses make their genome more difficult to construct than others. Two factors that affect the difficulty of infectious clone construction are genome length and genome complexity. Results This work examines the available strategies for overcoming the obstacles of assembling the long and complex RNA genomes of coronaviruses and reports one-step construction of an infectious clone system for the Middle East Respiratory Syndrome coronavirus (MERS-CoV) by homologous recombination in S. cerevisiae. Conclusions Future use of this methodology will shorten the time between emergence of a novel viral pathogen and construction of an infectious clone system. Completion of a viral infectious clone system facilitates further study of a virus’s biology, improvement of diagnostic tests, vaccine production and the screening of antiviral compounds.
has issue date	2016-10-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2016.07.022
bibo:pmid	27459876
has license	els-covid
sha1sum (hex)	bea1d1d130b407ba7382f561d3d193f6d9d646c7
schema:url	https://doi.org/10.1016/j.jviromet.2016.07.022
resource representing a document's title	Rapid one-step construction of a Middle East Respiratory Syndrome (MERS-CoV) infectious clone system by homologous recombination
has PubMed Central identifier	PMC7113859
has PubMed identifier	27459876
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:bea1d1d130b407ba7382f561d3d193f6d9d646c7#body_text
is schema:about of	named entity 'RAPID' named entity 'CONSTRUCTION' named entity 'shorten' named entity 'clone' named entity 'families' named entity 'Middle East Respiratory Syndrome coronavirus' named entity 'system' named entity 'production' named entity 'complexity' named entity 'IMPROVEMENT' named entity 'VIRAL GENOME' named entity 'DIFFICULTY' named entity 'ANIMAL' named entity 'VIRAL' named entity 'BIOLOGY' named entity 'ASSEMBLING' named entity 'ACROSS' named entity 'SYSTEMS' named entity 'SCREENING' named entity 'VACCINES' named entity 'REPORTS' named entity 'SHORTEN' named entity 'RESULTS' named entity 'LONG' named entity 'GENOME' named entity 'STRATEGIES' named entity 'NOVEL' named entity 'HAVE' named entity 'MOLECULAR' named entity 'AVAILABLE' named entity 'COMPLEX' named entity 'DIFFICULT' named entity '27S' named entity 'FUNCTION' named entity 'GENOMES' named entity 'ROBUST' named entity 'DEVELOPMENT' named entity 'USE OF' named entity 'WORK' named entity 'THEIR' named entity 'RNA' named entity 'STEP' named entity 'INFECTIOUS CLONE' named entity 'CONSTRUCT' named entity 'HOMOLOGOUS RECOMBINATION' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'SYSTEM' named entity 'IN S' named entity 'FAMILIES' named entity 'S. CEREVISIAE' named entity 'VIRAL GENE' named entity 'VIRUS' named entity 'INFECTIOUS CLONE' named entity 'VACCINE PRODUCTION' named entity 'POLIOVIRUS' named entity 'LENGTH' named entity 'BACKGROUND' named entity 'STEP' named entity 'MIDDLE EAST RESPIRATORY SYNDROME ' named entity 'HOMOLOGOUS RECOMBINATION' named entity 'GENOME COMPLEXITY' named entity 'SERVE' named entity 'STUDY' named entity 'METHODOLOGY' named entity 'TIME' named entity 'CONSTRUCTION' named entity 'ALLOWED' named entity 'COMPLETION' named entity 'AFFECT' named entity 'FUTURE' named entity 'CORONAVIRUSES' named entity 'HOST' named entity 'VIRUSES' named entity 'EMERGENCE' named entity 'WILD TYPE' named entity 'ADAPT'

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