About: In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila

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About: In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila
Creator	Chan, C Chen, Yiwei Wong, S Chan, H Chan, Ming Chan, Paul Fung, Tsui, Stephen Waye, Mary Chui, Y Kwok, Pui
Source	Elsevier; Medline; PMC
abstract	Abstract The Severe Acute Respiratory Syndrome-Coronavirus (SARS-CoV) 3a locus encodes a 274 a.a. novel protein, and its expression has been confirmed in SARS patients. To study functional roles of 3a, we established a transgenic fly model for the SARS-CoV 3a gene. Misexpression of 3a in Drosophila caused a dominant rough eye phenotype. Using a specific monoclonal antibody, we demonstrated that the 3a protein displayed a punctate cytoplasmic localization in Drosophila as in SARS-CoV-infected cells. We provide genetic evidence to support that 3a is functionally related to clathrin-mediated endocytosis. We further found that 3a misexpression induces apoptosis, which could be modulated by cellular cytochrome c levels and caspase activity. From a forward genetic screen, 78 dominant 3a modifying loci were recovered and the identity of these modifiers revealed that the severity of the 3a-induced rough eye phenotype depends on multiple cellular processes including gene transcriptional regulation.
has issue date	2005-11-18(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2005.09.098
bibo:pmid	16212942
has license	els-covid
sha1sum (hex)	c0e6a4d852babb843d63198cc1e16146efc108b1
schema:url	https://doi.org/10.1016/j.bbrc.2005.09.098
resource representing a document's title	In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila
has PubMed Central identifier	PMC7117541
has PubMed identifier	16212942
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:c0e6a4d852babb843d63198cc1e16146efc108b1#body_text
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