About: Oncolytic Reovirus Infection Is Facilitated by the Autophagic Machinery

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Oncolytic Reovirus Infection Is Facilitated by the Autophagic Machinery
Creator	Kemp, Vera Dautzenberg, Iris Hoeben, Rob Limpens, Ronald Van Den Wollenberg, Diana
Source	Medline; PMC
abstract	Mammalian reovirus is a double-stranded RNA virus that selectively infects and lyses transformed cells, making it an attractive oncolytic agent. Despite clinical evidence for anti-tumor activity, its efficacy as a stand-alone therapy remains to be improved. The success of future trials can be greatly influenced by the identification and the regulation of the cellular pathways that are important for reovirus replication and oncolysis. Here, we demonstrate that reovirus induces autophagy in several cell lines, evident from the formation of Atg5-Atg12 complexes, microtubule-associated protein 1 light chain 3 (LC3) lipidation, p62 degradation, the appearance of acidic vesicular organelles, and LC3 puncta. Furthermore, in electron microscopic images of reovirus-infected cells, autophagosomes were observed without evident association with viral factories. Using UV-inactivated reovirus, we demonstrate that a productive reovirus infection facilitates the induction of autophagy. Importantly, knock-out cell lines for specific autophagy-related genes revealed that the expression of Atg3 and Atg5 but not Atg13 facilitates reovirus replication. These findings highlight a central and Atg13-independent role for the autophagy machinery in facilitating reovirus infection and contribute to a better understanding of reovirus-host interactions.
has issue date	2017-09-21(xsd:dateTime)
bibo:doi	10.3390/v9100266
bibo:pmid	28934149
has license	cc-by
sha1sum (hex)	c1a54ec890c135c78fb0c2b0b9c1b96ac2b945eb
schema:url	https://doi.org/10.3390/v9100266
resource representing a document's title	Oncolytic Reovirus Infection Is Facilitated by the Autophagic Machinery
has PubMed Central identifier	PMC5691618
has PubMed identifier	28934149
schema:publication	Viruses
resource representing a document's body	covid:c1a54ec890c135c78fb0c2b0b9c1b96ac2b945eb#body_text
is schema:about of	named entity 'autophagy' named entity 'FBS' named entity 'selectively' named entity 'cell lines' named entity 'oncolytic' named entity 'All' covid:arg/c1a54ec890c135c78fb0c2b0b9c1b96ac2b945eb named entity 'CELLULAR' named entity 'AGENT' named entity 'HOST' named entity 'ACTIVITY' named entity 'TRANSFORMED' named entity 'ONCOLYTIC' named entity 'P62' named entity 'REVEALED' named entity 'CULTURED' named entity 'CLINICAL' named entity 'STAND' named entity 'LYSES' named entity 'PREVIOUSLY' named entity 'IMPORTANT' named entity 'TUMOR' named entity 'HERE' named entity 'LITERATURE' named entity 'ATTRACTIVE' named entity 'AUTOPHAGY-RELATED GENES' named entity 'PEN' named entity 'KNOCK-OUT' named entity 'ATG13' named entity 'EFFICACY' named entity 'IDENTIFICATION' named entity 'IMPROVED' named entity 'BETTER' named entity 'ITS' named entity 'USING' named entity 'ATG5' named entity 'MAINTAINED' named entity 'PATHWAYS' named entity 'FACTORIES' named entity 'COMPLEXES' named entity 'FINDINGS' named entity 'STREP' named entity 'DOUBLE-STRANDED RNA VIRUS' named entity 'FUTURE' named entity 'SPECIFIC' named entity 'FORMATION' named entity 'INDEPENDENT' named entity 'VIRAL' named entity 'AUTOPHAGY' named entity 'MAMMALIAN REOVIRUS' named entity 'INFECTS' named entity 'DEGRADATION' named entity 'CENTRAL' named entity 'LC3' named entity 'VESICULAR' named entity 'MEFS' named entity 'ATMOSPHERE' named entity 'CONTRIBUTE ' named entity 'MICROSCOPIC' named entity 'INDUCTION' named entity 'REOVIRUS INFECTION' named entity 'ELECTRON' named entity 'ATG3' named entity 'MACHINERY' named entity 'ACIDIC' named entity 'PRODUCTIVE' named entity 'ORGANELLES' named entity 'APPEARANCE' named entity '10%' named entity 'CELL LINES' named entity 'IMAGES' named entity 'THESE' named entity 'AUTOPHAGOSOMES' named entity 'GREATLY' named entity 'REGULATION' named entity 'INACTIVATED' named entity 'SUCCESS'

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