About: 600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus

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About: 600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus
Creator	Barnes, Ronald Ibey, Bennett Klein, Savannah Martens, Stacey Roth, Caleb Trejosanchez, Patricia
Source	PMC
abstract	Cell suspensions of Escherichia coli and Lactobacillus acidophilus were exposed to 600-ns pulsed electric fields (nsPEFs) at varying amplitudes (Low-13.5, Mid-18.5 or High-23.5 kV cm(−1)) and pulse numbers (0 (sham), 1, 5, 10, 100 or 1000) at a 1 hertz (Hz) repetition rate. The induced temperature rise generated at these exposure parameters, hereafter termed thermal gradient, was measured and applied independently to cell suspensions in order to differentiate inactivation triggered by electric field (E-field) from heating. Treated cell suspensions were plated and cellular inactivation was quantified by colony counts after a 24-hour (h) incubation period. Additionally, cells from both exposure conditions were incubated with various antibiotic-soaked discs to determine if nsPEF exposure would induce changes in antibiotic susceptibility. Results indicate that, for both species, the total delivered energy (amplitude, pulse number and pulse duration) determined the magnitude of cell inactivation. Specifically, for 18.5 and 23.5 kV cm(−1) exposures, L. acidophilus was more sensitive to the inactivation effects of nsPEF than E. coli, however, for the 13.5 kV cm(−1) exposures E. coli was more sensitive, suggesting that L. acidophilus may need to meet an E-field threshold before significant inactivation can occur. Results also indicate that antibiotic susceptibility was enhanced by multiple nsPEF exposures, as observed by increased zones of growth inhibition. Moreover, for both species, a temperature increase of ≤ 20 °C (89% of exposures) was not sufficient to significantly alter cell inactivation, whereas none of the thermal equivalent exposures were sufficient to change antibiotic susceptibility categories.
has issue date	2020-03-18(xsd:dateTime)
bibo:doi	10.1186/s13568-020-00991-y
bibo:pmid	32189137
has license	cc-by
sha1sum (hex)	c39ef825050419db56a87e17959c13f497ec48dc
schema:url	https://doi.org/10.1186/s13568-020-00991-y
resource representing a document's title	600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus
has PubMed Central identifier	PMC7080936
has PubMed identifier	32189137
schema:publication	AMB Express
resource representing a document's body	covid:c39ef825050419db56a87e17959c13f497ec48dc#body_text
is schema:about of	named entity 'susceptibility' named entity 'discs' named entity 'suspensions' named entity 'differentiate' named entity 'Lactobacillus acidophilus' named entity 'BY INCREASED' named entity '28H' named entity 'DIFFERENTIATE' named entity 'TEMPERATURE INCREASE' named entity 'NEED' named entity 'COUNTS' named entity 'LACTOBACILLUS ACIDOPHILUS' named entity 'PULSE DURATION' named entity 'NUMBERS' named entity 'TO CHANGE' named entity 'INACTIVATION' named entity 'PULSED' named entity 'SIGNIFICANT' named entity 'ANTIBIOTIC SUSCEPTIBILITY' named entity 'MAGNITUDE' named entity 'RISE' named entity 'THERMAL GRADIENT' named entity 'numbers' named entity 'multiple' named entity 'E-field' named entity 'pulse' named entity 'cells' named entity 'conditions' named entity 'amplitudes' named entity 'susceptibility' named entity 'species' named entity 'heating' named entity 'pulse' named entity 'number' named entity 'High' named entity 'antibiotic' named entity 'electric field' named entity 'Escherichia coli' named entity 'Lactobacillus acidophilus' named entity 'E. coli' named entity 'E. coli' named entity 'antibiotic susceptibility' named entity 'L. acidophilus' named entity 'Escherichia coli' named entity 'E-field' named entity 'L. acidophilus' named entity 'antibiotic susceptibility' named entity 'antibiotic' named entity 'bacterial cell wall' named entity 'bacteria' named entity 'NaCl' named entity 'incubation' named entity 'serial dilution' named entity 'MRS' named entity 'E-field' named entity '4a-c' named entity 'K-type' named entity 'Gram negative' named entity 'log reduction' named entity 'PEF' named entity 'antimicrobial agents' named entity 'biofilm' named entity '13.5' named entity 'antibiotics' named entity 'thermal gradient' named entity 'molecular dynamic simulations' named entity '13.5' named entity 'NaCl' named entity 'saline' named entity 'VWR International' named entity 'S. aureus' named entity 'PEF' named entity 'log reduction' named entity 'kanamycin' named entity 'optical density' named entity 'E-field'

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