About: Establishment and application of a real-time loop-mediated isothermal amplification system for the detection of CYP2C19 polymorphisms

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About: Establishment and application of a real-time loop-mediated isothermal amplification system for the detection of CYP2C19 polymorphisms Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Establishment and application of a real-time loop-mediated isothermal amplification system for the detection of CYP2C19 polymorphisms
Creator	Zhang, Chao Yao, Yao Cui, Ya-Li Hui, Wen-Li Wei, Hua Zhang, Shan-Shan Zhang, Si-Nong Zhu, Juan-Li
Source	PMC
abstract	Single-nucleotide polymorphisms (SNPs) represent the most widespread type of genetic variation (approximately 90%) in the human genome, and the demand to overcome such variation has received more attention now than ever before. The capacity to rapidly assess SNPs that correlate with disease predisposition, drug efficacy and drug toxicity is a key step for the development of personalized medicine. In this work, a rapid one-step SNP detection method, real-time loop-mediated isothermal amplification (RT-LAMP), was first applied for CYP2C19 polymorphisms testing. The optimized method was established with specifically designed primers for target amplification by real-time detection in approximately 30 min under isothermal conditions. RT-LAMP amplified few copies of template to produce significant amounts of product and quantitatively detected human DNA with compatible specificity and sensitivity. The success in the establishment of this RT-LAMP protocol for CYP2C19 polymorphism testing is significant for the extension of this technique for the detection of other SNPs, which will further facilitate the development of personalized medicine.
has issue date	2016-06-01(xsd:dateTime)
bibo:doi	10.1038/srep26533
bibo:pmid	27246657
has license	cc-by
sha1sum (hex)	c5df32e01e9583e1b4430418e8f8f58c8a85021c
schema:url	https://doi.org/10.1038/srep26533
resource representing a document's title	Establishment and application of a real-time loop-mediated isothermal amplification system for the detection of CYP2C19 polymorphisms
has PubMed Central identifier	PMC4887897
has PubMed identifier	27246657
schema:publication	Sci Rep
resource representing a document's body	covid:c5df32e01e9583e1b4430418e8f8f58c8a85021c#body_text
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