About: A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting in Drosophila Follicular Somatic Cells

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About: A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting in Drosophila Follicular Somatic Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting in Drosophila Follicular Somatic Cells
Creator	Imler, Jean-Luc Lemoine, Aurélie Martins, Nelson Meignin, Carine Paro, Simona Santiago, Estelle
Source	Medline; PMC
abstract	The small interfering RNA (siRNA) pathway is the main and best studied invertebrate antiviral response. Other poorly characterized protein based antiviral mechanisms also contribute to the control of viral replication in insects. In addition, it remains unclear whether tissue specific factors contribute to RNA and protein-based antiviral immunity mechanisms. In vivo screens to identify such factors are challenging and time consuming. In addition, the scored phenotype is usually limited to survival and/or viral load. Transgenic viral replicons are valuable tools to overcome these limitations and screen for novel antiviral factors. Here we describe transgenic Drosophila melanogaster lines encoding a Flock House Virus-derived replicon (FHV∆B2eGFP), expressing GFP as a reporter of viral replication. This replicon is efficiently controlled by the siRNA pathway in most somatic tissues, with GFP fluorescence providing a reliable marker for the activity of antiviral RNAi. Interestingly, in follicular somatic cells (FSC) of ovaries, this replicon is still partially repressed in an siRNA independent manner. We did not detect replicon derived Piwi-interacting RNAs in FSCs and identified 31 differentially expressed genes between restrictive and permissive FSCs. Altogether, our results uncovered a yet unidentified RNAi-independent mechanism controlling FHV replication in FSCs of ovaries and validate the FHV∆B2eGFP replicon as a tool to screen for novel tissue specific antiviral mechanisms.
has issue date	2018-12-12(xsd:dateTime)
bibo:doi	10.1534/g3.118.200872
bibo:pmid	30530643
has license	cc-by
sha1sum (hex)	c601e38de42c9188e237fe848d26c4a7c07c3d58
schema:url	https://doi.org/10.1534/g3.118.200872
resource representing a document's title	A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting in Drosophila Follicular Somatic Cells
has PubMed Central identifier	PMC6385967
has PubMed identifier	30530643
schema:publication	G3 (Bethesda)
resource representing a document's body	covid:c601e38de42c9188e237fe848d26c4a7c07c3d58#body_text
is schema:about of	named entity 'DROSOPHILA' named entity 'tool' named entity 'efficiently' named entity 'survival' named entity 'validate' named entity 'ovaries' named entity 'replicons' named entity 'replicon' named entity 'somatic cells' named entity 'FLOCK HOUSE VIRUS' named entity 'REVEALS' named entity 'SOMATIC CELLS' named entity 'TOOLS' named entity 'PROTEIN ' named entity 'RNA' named entity 'INDEPENDENT' named entity 'OVARIES' named entity 'VIRAL' named entity 'REPLICON' named entity 'POORLY' named entity 'CONSUMING' named entity 'DID' named entity 'RESULTS' named entity 'FLOCK HOUSE VIRUS' named entity 'USUALLY' named entity 'TRANSGENIC' named entity 'MAIN' named entity 'PARTIALLY' named entity 'SMALL INTERFERING RNA' named entity 'IDENTIFIED' named entity 'VIRAL REPLICATION' named entity 'REPORTER' named entity 'RNAI' named entity 'OVERCOME' named entity 'UNCLEAR' named entity 'CONTROLLING' named entity 'ACTIVITY' named entity 'INVERTEBRATE' named entity 'BASED' named entity 'EXPRESSED' named entity 'MARKER' named entity 'THESE' named entity 'CONTRIBUTE ' named entity 'PROVIDING' named entity 'CONTROLLED BY' named entity 'RELIABLE' named entity 'DERIVED' named entity 'OUR' named entity 'LINES' named entity 'PIWI' named entity 'INTERACTING' named entity 'PHENOTYPE' named entity 'SOMATIC CELLS' named entity 'MECHANISM' named entity 'ANTIVIRAL' named entity 'UNCOVERED' named entity 'UNIDENTIFIED' named entity 'SOMATIC' named entity 'GENES' named entity 'SURVIVAL' named entity 'SCREEN' named entity 'TO IDENTIFY' named entity 'FLUORESCENCE' named entity 'INSECTS' named entity 'REPRESSED' named entity 'SCORED' named entity 'REPLICATION' named entity 'PATHWAY' named entity 'IN VIVO' named entity 'FOLLICULAR' named entity 'CONTROL' named entity 'TRANSGENIC' named entity 'FOLLICULAR' named entity 'REPLICON' named entity 'INDEPENDENT' named entity 'RNAI'

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