About: Novel replication complex architecture in rubella replicon‐transfected cells

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About: Novel replication complex architecture in rubella replicon‐transfected cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Novel replication complex architecture in rubella replicon‐transfected cells
Creator	Fontana, Juan Tzeng, Wen-Pin Risco, Cristina Frey, Teryl Calderita, Gloria Fraile-Ramos, Alberto
source	Medline; PMC
abstract	Rubella virus (RUB) assembles its replication complexes (RCs) in modified organelles of endo‐lysosomal origin, known as cytopathic vacuoles (CPVs). These peculiar structures are key elements of RUB factories, where rough endoplasmic reticulum, mitochondria, and Golgi are recruited. Bicistronic RUB replicons expressing an antibiotic resistance gene either in the presence or the absence of the RUB capsid (C) gene were used to study the structure of RCs in transfected cells. Confocal microscopy showed that the RUB replicase components P90 and P150 localized to CPVs, as did double‐stranded RNA (dsRNA), a marker for RNA synthesis. Electron microscopy (EM) showed that replicons generated CPVs containing small vesicles and large vacuoles, similar to CPVs from RUB‐infected cells and that the replicase proteins were sufficient for organelle recruitment. Some of these CPVs contained straight membranes. When cross‐sectioned, these rigid membranes appeared to be sheets of closely packed proteins. Immuno‐EM revealed that these sheets, apparently in contact with the cytosol, contained both P150 and P90, as well as dsRNA, and thus could be two‐dimensional arrays of functional viral replicases. Labelling of dsRNA after streptolysin‐O permeabilization showed that replication of viral genome takes place on the cytoplasmic side of CPVs. When present, C accumulated around CPVs. Mitochondrial protein P32 was detected within modified CPVs, the first demonstration of involvement of this protein, which interacts with C, with RCs.
has issue date	2006-10-18(xsd:dateTime)
bibo:doi	10.1111/j.1462-5822.2006.00837.x
bibo:pmid	17087733
has license	no-cc
sha1sum (hex)	c636ab720f539ae69aef666b1d2f337aca976f05
schema:url	https://doi.org/10.1111/j.1462-5822.2006.00837.x
resource representing a document's title	Novel replication complex architecture in rubella replicon‐transfected cells
has PubMed Central identifier	PMC7162162
has PubMed identifier	17087733
schema:publication	Cell Microbiol
resource representing a document's body	covid:c636ab720f539ae69aef666b1d2f337aca976f05#body_text
is schema:about of	named entity 'RNA' named entity 'cells' named entity 'structures' named entity 'Labelling' named entity 'recruited' named entity 'P90' named entity 'presence' named entity 'labelling' named entity 'replicons' named entity 'Novel' named entity 'viral' named entity 'labelling' named entity 'maintained' named entity 'proteins' named entity 'Electron microscopy' named entity 'expressing' named entity 'recruitment' named entity 'Golgi' named entity 'denoted' named entity 'rigid' named entity 'transfected' named entity 'Association' named entity 'arrowheads' named entity 'antibodies' named entity 'factories' named entity 'arrays' named entity 'permeabilization' named entity 'proteins' named entity 'straight' named entity 'GFP' named entity 'two-dimensional' named entity 'dsRNA' named entity 'asterisks' named entity 'interacts' named entity 'structure' named entity 'mitochondria' named entity 'GFP' named entity 'replicases' named entity 'replicase' named entity 'transfected' named entity 'antibiotic resistance' named entity 'replicons' named entity 'capsid' named entity 'mitochondria' named entity 'cytoplasmic' named entity 'capsid' named entity 'replicase' named entity 'Mitochondrial' named entity 'vacuoles' named entity 'organelle' named entity 'permeabilization' named entity 'SLO' named entity 'protein' named entity 'Immunogold labelling' named entity 'dsRNA' named entity 'dsRNA' named entity 'replicase' named entity 'permeabilization' named entity 'cells' named entity 'cytosol' named entity 'antibodies' named entity 'replicons' named entity 'viral genome' named entity 'IRES' named entity 'GFP' named entity 'RNA viruses' named entity 'saponin' named entity 'microscopy' named entity 'Fluorescent' named entity 'life cycle' named entity 'gene' named entity 'PCR-amplified' named entity 'actin microfilaments' named entity 'virus' named entity 'monoclonal antibody' named entity 'BHK-21 cells'

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