About: Novel avian single-chain fragment variable (scFv) targets dietary gluten and related natural grain prolamins, toxic entities of celiac disease

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Novel avian single-chain fragment variable (scFv) targets dietary gluten and related natural grain prolamins, toxic entities of celiac disease Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Novel avian single-chain fragment variable (scFv) targets dietary gluten and related natural grain prolamins, toxic entities of celiac disease
Creator	Forster, Florian Harant, Hanna Hermann, Marcela Korschineck, Irina Missbichler, Albert Stadlmann, Valerie
Source	Medline; PMC
abstract	BACKGROUND: Celiac disease (CD) is a chronic, small intestinal inflammatory disease mediated by dietary gluten and related prolamins. The only current therapeutic option is maintenance of a strict life-long gluten-free diet, which implies substantial burden for CD patients. Different treatment regimes might be feasible, including masking of toxic celiac peptides with blocking antibodies or fragments thereof. The objective of this study was therefore to select and produce a recombinant avian single-chain fragment variable (scFv) directed against peptic-tryptic digested gliadin (PT-Gliadin) and related celiac toxic entities. RESULTS: Gluten-free raised chicken of same age were immunized with PT-Gliadin. Chicken splenic lymphocytes, selected with antigen-coated magnetic beads, served as RNA source for the generation of cDNA. Chicken V(H) and V(L) genes were amplified from the cDNA by PCR to generate full-length scFv constructs consisting of V(H) and V(L) fragments joined by a linker sequence. ScFv constructs were ligated in a prokaryotic expression vector, which provides a C-terminal hexahistidine tag. ScFvs from several bacterial clones were expressed in soluble form and crude cell lysates screened for binding to PT-Gliadin by ELISA. We identified an enriched scFv motif, which showed reactivity to PT-Gliadin. One selected scFv candidate was expressed and purified to homogeneity. Polyclonal anti-PT-Gliadin IgY, purified from egg yolk of immunized chicken, served as control. ScFv binds in a dose-dependent manner to PT-Gliadin, comparable to IgY. Furthermore, IgY competitively displaces scFv from PT-Gliadin and natural wheat flour digest, indicating a common epitope of scFv and IgY. ScFv was tested for reactivity to different gastric digested dietary grain flours. ScFv detects common and khorasan wheat comparably with binding affinities in the high nanomolar range, while rye is detected to a lesser extent. Notably, barley and cereals which are part of the gluten-free diet, like corn and rice, are not detected by scFv. Similarly, the pseudo-grain amaranth, used as gluten-free alternative, is not targeted by scFv. This data indicate that scFv specifically recognizes toxic cereal peptides relevant in CD. CONCLUSION: ScFv can be of benefit for future CD treatment regimes.
has issue date	2015-12-01(xsd:dateTime)
bibo:doi	10.1186/s12896-015-0223-z
bibo:pmid	26625857
has license	cc-by
sha1sum (hex)	c75ed4c49d52ac5862313eb9d22cad197df1c138
schema:url	https://doi.org/10.1186/s12896-015-0223-z
resource representing a document's title	Novel avian single-chain fragment variable (scFv) targets dietary gluten and related natural grain prolamins, toxic entities of celiac disease
has PubMed Central identifier	PMC4666168
has PubMed identifier	26625857
schema:publication	BMC Biotechnol
resource representing a document's body	covid:c75ed4c49d52ac5862313eb9d22cad197df1c138#body_text
is schema:about of	named entity 'TOXIC' named entity 'gliadin' named entity 'entities' named entity 'study' named entity 'SINGLE' named entity 'AVIAN' covid:arg/c75ed4c49d52ac5862313eb9d22cad197df1c138 named entity 'CHAIN' named entity 'LONG' named entity 'SELECT' named entity 'MIGHT BE' named entity 'GLIADIN' named entity 'INFLAMMATORY DISEASE' named entity 'BURDEN' named entity 'SINGLE' named entity 'DIFFERENT' named entity 'BLOCKING ANTIBODIES' named entity 'IS A' named entity 'OPTION' named entity 'GLUTEN-FREE DIET' named entity 'TREATMENT' named entity 'FRAGMENT' named entity 'INCLUDING' named entity 'PRODUCE' named entity 'CURRENT' named entity 'VARIABLE' named entity 'MEDIATED' named entity 'DIGESTED' named entity 'CHRONIC' named entity 'PEPTIDES' named entity 'THERAPEUTIC' named entity 'DIRECTED' named entity 'TOXIC' named entity 'CELIAC DISEASE' named entity 'RELATED' named entity 'NATURAL' named entity 'VARIABLE' named entity 'PROLAMINS' named entity 'GRAIN' named entity 'OBJECTIVE' named entity 'PROLAMINS' named entity 'CHAIN' named entity 'PEPTIC' named entity 'STUDY' named entity 'CELIAC DISEASE' named entity 'FRAGMENTS' named entity 'DIETARY' named entity 'BACKGROUND' named entity 'RECOMBINANT' named entity 'CELIAC' named entity 'AVIAN' named entity 'LIFE' named entity 'NOVEL' named entity 'GLUTEN' named entity 'FRAGMENT' named entity 'FEASIBLE' named entity 'MASKING' named entity 'TARGETS' named entity 'DIETARY' named entity 'PATIENTS' named entity 'GLUTEN' named entity 'MAINTENANCE' named entity 'SMALL INTESTINAL' named entity 'RELATED' named entity 'gluten-free diet' named entity 'gluten' named entity 'option' named entity 'antibodies' named entity 'blocking' named entity 'digested' named entity 'variable' named entity 'small intestinal' named entity 'mediated' named entity 'patients' named entity 'gluten' named entity 'peptides'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software