About: Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)

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About: Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)
Creator	Hofmann, Martin Wyler, Robert
source	Elsevier; Medline; PMC
abstract	Abstract The porcine epidemic coronavirus (PEDV), tentatively classified as a coronavirus, was adapted to Vero cells and a plaque test developed for infectivity titration, allowing us to test the biological and biophysical properties of the virus. Growth kinetics showed peak titers of 105.5 plaque-forming units ml−1 15 h after infection. Filtration experiments and electron microscopy revealed a particle diameter between 100 and 200 nm. The bouyant density of the virus was 1.18. The particle lost its infectivity on treatment with lipid solvents. Virus replication could not be inhibited by 5-iodo-2′-deoxyuridine. PEDV was moderately stable at 50°C, but heat sensitivity was not altered by divalent cations. At 4°C, the virus was stable between pH 5.0 and 9.0, but at 37°C stability was restricted to the pH range 6.5–7.5. Viral infectivity was not impaired by ultrasonication or by multiple freezing and thawing. PEDV was not neutralized by transmissible gastroenteritis virus antiserum. On the basis of the tests carried out, PEDV is a pleomorphic, enveloped RNA virus with a particle diameter of ∼ 150 nm and a bouyant density of 1.18. Infectivity depends on the presence of trypsin, and infected cells show a tendency to fuse and to form syncytia. All of these properties, as well as its physicochemical characteristics, allow PEDV to be classified as a coronavirus.
has issue date	1989-06-30(xsd:dateTime)
bibo:doi	10.1016/0378-1135(89)90036-9
bibo:pmid	2549681
has license	els-covid
sha1sum (hex)	c86d17519bdf0baf3703ab2776dc60f3677be5cc
schema:url	https://doi.org/10.1016/0378-1135%2889%2990036-9
resource representing a document's title	Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)
has PubMed Central identifier	PMC7117183
has PubMed identifier	2549681
schema:publication	Veterinary Microbiology
resource representing a document's body	covid:c86d17519bdf0baf3703ab2776dc60f3677be5cc#body_text
is schema:about of	named entity 'transmissible' named entity 'developed' named entity 'neutralized' named entity 'cells' named entity 'trypsin' named entity 'Microbiol.' named entity 'porcine' named entity 'fuse' named entity 'stability' named entity 'test' named entity 'diarrhea' named entity 'Cell' named entity 'ADAPTED' named entity 'TRYPSIN' named entity 'TITRATION' named entity 'BIOLOGICAL' named entity 'QUANTITATION' named entity 'INFECTION' named entity 'PLEOMORPHIC' named entity 'PEDV' named entity 'ADAPTED' named entity 'TO FORM' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity '100' named entity 'ELECTRON MICROSCOPY' named entity 'BIOPHYSICAL' named entity 'PORCINE EPIDEMIC DIARRHEA' named entity 'SOLVENTS' named entity 'ITS' named entity 'FREEZING' covid:arg/c86d17519bdf0baf3703ab2776dc60f3677be5cc named entity 'VIRAL' named entity '50%' named entity 'ANTISERUM' named entity 'TENDENCY' named entity 'PORCINE EPIDEMIC DIARRHEA' named entity 'BIOLOGICAL' named entity 'CELL CULTURE' named entity 'CORONAVIRUS' named entity 'QUANTITATION' named entity 'PHYSICOCHEMICAL PROPERTIES' named entity 'PARTICLE' named entity 'THESE' named entity '150' named entity 'PH 5.0' named entity 'SYNCYTIA' named entity 'ALLOW' named entity 'LOST' named entity 'STABILITY' named entity 'PRESENCE OF' named entity 'CELL CULTURE' named entity 'PEAK' named entity 'REVEALED' named entity 'EPIDEMIC' named entity 'CARRIED' named entity 'CLASSIFIED' named entity 'CHARACTERISTICS' named entity 'LIPID' named entity 'PHYSICOCHEMICAL' named entity 'EXPERIMENTS' named entity 'DEOXYURIDINE' named entity 'IODO' named entity 'MODERATELY' named entity 'PARTICLE DIAMETER' named entity 'ALTERED' named entity 'STABLE' named entity 'RNA VIRUS' named entity 'TEST' named entity 'GROWTH KINETICS' named entity 'INFECTED' named entity 'PH RANGE' named entity 'HEAT SENSITIVITY' named entity 'THAWING' named entity 'PLAQUE' named entity 'ULTRASONICATION' named entity 'MULTIPLE' named entity 'ALLOWING' named entity 'CORONAVIRUS' named entity 'DENSITY' named entity 'FILTRATION'

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