About: Deployable, Field-Sustainable, Reverse Transcription-Polymerase Chain Reaction Assays for Rapid Screening and Serotype Identification of Dengue Virus in Mosquitoes

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Deployable, Field-Sustainable, Reverse Transcription-Polymerase Chain Reaction Assays for Rapid Screening and Serotype Identification of Dengue Virus in Mosquitoes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Deployable, Field-Sustainable, Reverse Transcription-Polymerase Chain Reaction Assays for Rapid Screening and Serotype Identification of Dengue Virus in Mosquitoes
Creator	Col, Lt Col, ; Blow, Jamie Huff, William Maj, ; Mcavin, James Powers, Michael Putnam, John Swaby, James
topic	covid:cd1b2f699db69f95711d8621765f70f9abf25dac#this
source	PMC
abstract	Dengue virus universal and serotype 1 to 4 fluorogenic probe hydrolysis, reverse transcription (RT)-polymerase chain reaction (PCR) assays and positive-control RNA template were freeze-dried in a thermally stable, hydrolytic enzyme-resistant format and deployed for testing in a dengue fever-endemic region of Thailand. The study site presented austere testing conditions. Field-collected Aedes aegypti mosquitoes spiked with inoculated A. aegypti mosquitoes and individual and pooled, field-collected, A. aegypti, A. albopictus, and Culex tritaeniorhynchus mosquitoes were used for RT-PCR assay evaluations. For dengue virus-inoculated A. aegypti mosquitoes and spiked samples, in vitro sensitivity and specificity results for all five assays were concordant with indirect fluorescent antibody assay results. A single pool of field-collected, female, A. aegypti mosquitoes was identified as dengue virus positive. Cross-reactivity was not observed across heterologous serotypes, mosquito vectors, or human DNA. The limit of detection was >7 to ≤70 genomic equivalents. Sample processing and analysis required <2 hours. These results show promise of field-formatted RT-PCR reagents for rapid, sensitive, specific dengue virus screening and serotype identification in mosquitoes under field-deployed conditions.
has issue date	2007-03-01(xsd:dateTime)
bibo:doi	10.7205/milmed.172.3.329
bibo:pmid	17436782
has license	no-cc
sha1sum (hex)	cd1b2f699db69f95711d8621765f70f9abf25dac
schema:url	https://doi.org/10.7205/milmed.172.3.329
resource representing a document's title	Deployable, Field-Sustainable, Reverse Transcription-Polymerase Chain Reaction Assays for Rapid Screening and Serotype Identification of Dengue Virus in Mosquitoes
has PubMed Central identifier	PMC7110365
has PubMed identifier	17436782
schema:publication	Mil Med
resource representing a document's body	covid:cd1b2f699db69f95711d8621765f70f9abf25dac#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/cd1b2f699db69f95711d8621765f70f9abf25dac
is schema:about of	named entity 'DENGUE VIRUS' named entity 'spiked' named entity 'inoculated' named entity 'heterologous' named entity 'Serotype' named entity 'HUMAN' named entity 'ANALYSIS' named entity 'TESTING' named entity 'RT-PCR' named entity 'EVALUATIONS' named entity 'RESISTANT' named entity 'TEMPLATE' named entity 'POOLED' named entity 'DENGUE FEVER' named entity 'PRESENTED' named entity 'SENSITIVE' named entity 'PROBE' named entity 'THESE' named entity 'GENOMIC EQUIVALENTS' named entity 'MOSQUITO VECTORS' named entity 'CONTROL' named entity 'IDENTIFIED' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'RESULTS' named entity 'SEROTYPE' named entity 'POSITIVE' named entity 'PROMISE' named entity 'SAMPLES' named entity 'UNIVERSAL' named entity 'HOURS' named entity 'SINGLE' named entity 'REAGENTS' named entity 'CROSS' named entity 'REGION' named entity 'RT-PCR ASSAY' named entity 'FIELD' named entity 'REVERSE TRANSCRIPTION' named entity 'IDENTIFICATION' named entity 'RAPID SCREENING' named entity 'POLYMERASE CHAIN REACTION' named entity 'DEPLOYED' named entity 'FEMALE' named entity 'IN VITRO' named entity 'ENZYME' named entity 'INDIVIDUAL' named entity 'FLUORESCENT ANTIBODY ASSAY' named entity 'AEDES AEGYPTI' named entity 'FIELD' named entity 'DENGUE VIRUS' named entity 'POLYMERASE CHAIN REACTION' named entity 'CULEX TRITAENIORHYNCHUS' named entity 'COLLECTED' named entity 'RAPID' named entity 'IDENTIFICATION' named entity 'SAMPLE PROCESSING' named entity 'CONDITIONS' named entity 'HETEROLOGOUS' named entity 'NOT OBSERVED' named entity 'REVERSE TRANSCRIPTION' named entity 'DNA' named entity 'LIMIT OF DETECTION' named entity 'RNA' named entity 'MOSQUITOES' named entity 'FORMAT' named entity 'SCREENING' named entity 'SEROTYPES' named entity 'THAILAND' named entity 'BD2' named entity 'REACTIVITY' named entity 'USED' named entity 'STABLE'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software