About: Development and validation of a real-time PCR for the detection and quantification of porcine circovirus type 2

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About: Development and validation of a real-time PCR for the detection and quantification of porcine circovirus type 2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and validation of a real-time PCR for the detection and quantification of porcine circovirus type 2
Creator	Duarte, Margarida Fagulha, Teresa Fevereiro, Miguel Luís, Tiago Ramos, Fernanda Barros, Carla Margarida Henriques, Ana Sílvia, •
Source	Medline; PMC
abstract	Porcine circovirus type 2 (PCV2) is a spherical and non-enveloped virus belonging to the genus Circovirus of the Circoviridae family with a single stranded circular DNA genome. This virus, already detected worldwide, has been associated to several diseases and was implicated as the etiological agent of a disease named postweaning multisystemic wasting syndrome. Several methods have been described for the detection of PCV2, being real-time PCR the most simple and reliable. As far as we know, all the real-time PCR systems described until now are based on ORF2 gene, that exhibit the highest variability. This paper reports the development and validation of a real-time PCR targeted to ORF1 and based on a TaqMan probe for the detection of porcine circovirus type 2 DNA in swine samples. Due to the lack of PCV1 samples, the ability of the test to discriminate between PCV1 and PCV2 positive samples was evaluated in silico. Estimations of 100% specificity and 100% sensitivity were obtained based on the qPCR results with panel of 81 swine samples (known PCV2-positive (n = 50); known PCV2-negative (n = 17); samples positive to other common swine viral pathogens (n = 13) and one sample from a BFDV-positive parrot (n = 1)). Intra- and inter-assay coefficients of variation obtained with three positive samples of different viral charges in five replicates or in five independent assays were below the acceptance threshold. The limit of detection determined with a recombinant plasmid containing the amplicon, led to conclude that this assay can detect at least three plasmid copies.
has issue date	2018-07-17(xsd:dateTime)
bibo:doi	10.1007/s13337-018-0476-y
bibo:pmid	30159371
has license	bronze-oa
sha1sum (hex)	cd4798696373c4c672c4d9625811e5aae9ffce49
schema:url	https://doi.org/10.1007/s13337-018-0476-y
resource representing a document's title	Development and validation of a real-time PCR for the detection and quantification of porcine circovirus type 2
has PubMed Central identifier	PMC6111954
has PubMed identifier	30159371
schema:publication	VirusDisease
resource representing a document's body	covid:cd4798696373c4c672c4d9625811e5aae9ffce49#body_text
is schema:about of	named entity 'porcine circovirus' named entity 'negative' named entity 'determined' named entity 'specificity' named entity 'panel' named entity 'genus' named entity 'validation' named entity 'NAMED' named entity 'ORF1' named entity 'KNOWN' named entity 'CIRCOVIRIDAE' named entity 'SIMPLE' named entity 'DETERMINED' named entity 'RELIABLE' named entity 'ENVELOPED VIRUS' named entity 'GENUS' named entity 'LACK' named entity 'BEING' named entity 'NON-' named entity 'DETECT' named entity 'LIMIT OF DETECTION' named entity 'DUE TO' named entity 'SPHERICAL' named entity 'IS A' named entity 'INTRA' named entity 'DISEASE' named entity 'ASSOCIATED' named entity 'GENE' named entity 'TARGETED' named entity 'DISEASES' named entity 'SINGLE' named entity 'PORCINE CIRCOVIRUS TYPE 2' named entity 'HIGHEST' named entity 'BFDV' named entity 'OBTAINED' named entity 'ABILITY' named entity 'METHODS' named entity 'VALIDATION' named entity 'QUANTIFICATION' named entity 'SENSITIVITY' named entity '50%' named entity 'REPLICATES' named entity 'PLASMID' named entity 'LED' named entity 'DEVELOPMENT' named entity 'DETECTION' named entity 'REAL-TIME PCR ' named entity 'DETECTION' named entity 'COEFFICIENTS' named entity 'PROBE' named entity 'IN SILICO' named entity 'PANEL' named entity 'TEST' named entity 'GENOME' named entity 'CONTAINING' named entity 'DETECTED' named entity 'DISCRIMINATE' named entity 'ORF2' named entity 'INDEPENDENT' named entity 'NEGATIVE' named entity 'EXHIBIT' named entity 'VARIABILITY' named entity 'PORCINE CIRCOVIRUS TYPE 2' named entity 'BELONGING' named entity 'TAQMAN' named entity 'BASED' named entity 'VALIDATION' named entity 'CIRCULAR DNA' named entity 'REPORTS' named entity 'PORCINE CIRCOVIRUS TYPE 2 DNA' named entity '100%' named entity 'HAVE' named entity 'CHARGES' named entity 'WORLDWIDE'

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