About: A novel multiplex PCR-electronic microarray assay for rapid and simultaneous detection of bovine respiratory and enteric pathogens

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About: A novel multiplex PCR-electronic microarray assay for rapid and simultaneous detection of bovine respiratory and enteric pathogens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A novel multiplex PCR-electronic microarray assay for rapid and simultaneous detection of bovine respiratory and enteric pathogens
Creator	Ambagala, Aruna Furukawa-Stoffer, Tara Lung, Oliver Thanthrige-Don, Niroshan Alexander, Trevor Buchanan, Cody Gilleard, John Godson, Dale Joseph, Tomy
Source	Elsevier; Medline; PMC
abstract	Abstract Respiratory and enteric diseases continue to be two major causes of economic losses to the cattle industry worldwide. Despite their multifactorial etiology, the currently available diagnostic tests for bovine respiratory disease complex (BRDC) and bovine enteric disease (BED) are single-pathogen-tests. DNA microarray when combined with multiplex polymerase chain reaction (PCR) is a powerful tool in detection and differentiation of multiple pathogens in a single sample. This study reports development and initial validation of two independent highly sensitive and specific multiplex PCR-electronic microarray assays, one for the detection and differentiation of pathogens of the BRDC and the other for detection and differentiation of pathogens of the BED. The BRDC multiplex PCR-microarray assay was able to detect and differentiate four bacteria (Mannheimia haemolytica, Histophilus somni, Pasteurella multocida, and Mycoplasma bovis) and five viruses [bovine parainfluenza virus-3, bovine respiratory syncytial virus, bovine herpesvirus-1, bovine coronavirus (BCoV), and bovine viral diarrhea virus (BVDV)] associated with BRDC. The BED multiplex PCR- microarray- assay was able to detect and differentiate four bacteria (Clostridium perfringens, Escherichia coli, Salmonella enterica Dublin, and Salmonella enterica Typhimurium), three protozoa (Eimeria zuernii, Eimeria bovis, and Cryptosporidium parvum), and four viruses (bovine torovirus, bovine rotavirus, BCoV, and BVDV) associated with the BED. Both assays detected their respective targets individually or in combination when present. The limit-of-detection of each assay at the PCR amplification and DNA microarray levels was determined using previously titrated laboratory amplified target pathogens or using quantified synthetic nucleotides. Both assays showed very high analytical sensitivity and specificity, and were validated using a limited number of clinical samples. The BRDC and BED multiplex PCR- microarray-assays developed in this study, with further clinical validation, could be used in veterinary diagnostic laboratories for the rapid and simultaneous identification of pathogens to facilitate quick and accurate decision making for the control and treatment of these two economically important disease complexes. Furthermore, these assays could be very effective tools in epidemiological studies as well as for screening of healthy animals to identify carriers that may potentially develop BRDC or BED.
has issue date	2018-11-30(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2018.08.010
bibo:pmid	30102924
has license	els-covid
sha1sum (hex)	cdc208425eb355ce04e456a38a55b16f515cacff
schema:url	https://doi.org/10.1016/j.jviromet.2018.08.010
resource representing a document's title	A novel multiplex PCR-electronic microarray assay for rapid and simultaneous detection of bovine respiratory and enteric pathogens
has PubMed Central identifier	PMC7113860
has PubMed identifier	30102924
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:cdc208425eb355ce04e456a38a55b16f515cacff#body_text
is schema:about of	named entity 'ENTERIC' named entity 'RESPIRATORY' named entity 'bovine' named entity 'PATHOGENS' named entity 'RAPID' named entity 'MICROARRAY ASSAY' named entity 'NOVEL' named entity 'DETECTION' named entity 'SIMULTANEOUS' named entity 'BOVINE' named entity 'MULTIPLEX PCR' named entity 'ELECTRONIC' named entity 'pathogens' named entity 'respiratory' named entity 'bovine' named entity 'assay' named entity 'pathogens' named entity 'BVDV' named entity 'plasmids' named entity 'analyte' named entity 'pathogen' named entity 'PCR amplification' named entity 'Angen' named entity 'cloned' named entity 'Histophilus somni' named entity '37°C' named entity 'viruses' named entity 'Piscataway' named entity 'microarray' named entity 'pathogens' named entity 'VP6' named entity 'bovine' named entity 'virus' named entity 'pathogens' named entity 'microbes' named entity 'Manitoba' named entity 'fluorescent dyes' named entity 'protozoal' named entity 'pathogens' named entity 'Qiagen' named entity 'protozoa' named entity 'viral infections' named entity 'type 1' named entity 'pathogens' named entity 'multiplex PCR' named entity 'microarray' named entity 'Pasteurella multocida' named entity 'PCR' named entity 'pathogens' named entity 'Lung' named entity 'virus' named entity 'Saskatoon' named entity 'enteric diseases' named entity 'BRDC' named entity 'microarray' named entity '1, 2' named entity 'virulence factors' named entity '5′ end' named entity '37°C' named entity 'dengue virus' named entity 'diarrhea' named entity '5′-untranslated region' named entity 'Sudiro' named entity 'pathogen' named entity 'nucleic acids' named entity 'bacteria' named entity 'bacteria' named entity 'immunosuppression' named entity 'pathogens' named entity 'multiplex PCR' named entity 'enteric diseases' named entity 'Lung' named entity 'parasitic protozoans'

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