About: Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli

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About: Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli
Creator	Chien, Chang Lin, Loa, Ching Wu, Tsang
Source	PMC
abstract	Expression and purification of turkey coronavirus (TCoV) nucleocapsid (N) protein from a prokaryotic expression system as histidine-tagged fusion protein are presented in this chapter. Expression of histidine-tagged fusion N protein with a molecular mass of 57 kDa is induced with isopropyl β-d-1-thiogalactopyranoside (IPTG). The expressed N protein inclusion body is extracted and purified by chromatography on nickel-agarose column to near homogeneity. The protein recovery can be 10 mg from 100 ml of bacterial culture. The purified N protein is a superior source of TCoV antigen for antibody-capture ELISA for detection of antibodies to TCoV.
has issue date	2015-09-10(xsd:dateTime)
bibo:doi	10.1007/978-1-4939-3414-0_4
has license	no-cc
sha1sum (hex)	cfe97904fe4f96abb947b3480ec96b3e66b45c35
schema:url	https://doi.org/10.1007/978-1-4939-3414-0_4
resource representing a document's title	Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli
has PubMed Central identifier	PMC7122433
schema:publication	Animal Coronaviruses
resource representing a document's body	covid:cfe97904fe4f96abb947b3480ec96b3e66b45c35#body_text
is schema:about of	named entity 'Expression' named entity 'antigen' named entity 'protein' named entity 'induced' covid:arg/cfe97904fe4f96abb947b3480ec96b3e66b45c35 named entity 'extracted' named entity 'kDa' named entity 'inclusion body' named entity 'expression system' named entity 'isopropyl' named entity 'fusion protein' named entity 'protein' named entity 'nucleocapsid' named entity 'bacterial culture' named entity 'protein' named entity 'TCoV' named entity 'nucleocapsid' named entity 'nucleocapsid' named entity 'TCoV' named entity 'nickel' named entity 'IPTG' named entity 'prokaryotic' named entity 'molecular mass' named entity 'Nucleocapsid Protein' named entity 'protein recovery' named entity 'Turkey Coronavirus' named entity 'cation' named entity 'protein' named entity 'protein' named entity 'protein' named entity 'TCoV' named entity 'kDa' named entity 'inclusion body' named entity 'antigen' named entity 'Recombinant' named entity 'chromatography' named entity 'nucleocapsid' named entity 'histidine' named entity 'turkey coronavirus' named entity 'nucleocapsid' named entity 'antibodies' named entity 'Escherichia coli' named entity 'agarose' named entity 'antibodies' named entity 'IPTG' named entity 'cation' named entity 'nucleocapsid' named entity 'superior' named entity 'expressed' named entity 'fusion' named entity 'Chapter' named entity 'Recombinant' named entity 'Protein' named entity 'fusion protein' named entity 'detection' named entity 'Expression' named entity 'protein' named entity 'presented' named entity 'bacterial culture' named entity 'homogeneity' named entity 'chromatography' named entity 'Escherichia coli' named entity 'prokaryotic' named entity 'protein' named entity 'protein' named entity 'Turkey' named entity 'molecular mass' named entity 'Coronavirus'
is wasQuotedFrom of	covid:arg/cfe97904fe4f96abb947b3480ec96b3e66b45c35/0
is part of of	Expression and purification of turkey coronavirus (TCoV) nucleocapsid (N) protein from a prokaryotic expression system as histidine-tagged fusion protein are presented in this chapter. Expression of histidine-tagged fusion N protein with a molecular mass of 57 kDa is induced with isopropyl β-d-1-thiogalactopyranoside (IPTG). The expressed N protein inclusion body is extracted and purified by chromatography on nickel-agarose column to near homogeneity. The protein recovery can be 10 mg from 100 ml of bacterial culture. The purified N protein is a superior source of TCoV antigen for antibody-capture ELISA for detection of antibodies to TCoV. covid:cfe97904fe4f96abb947b3480ec96b3e66b45c35#body_text Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli

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