About: Identification of airborne bacteria by 16S rDNA sequencing, MALDI-TOF MS and the MIDI microbial identification system

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About: Identification of airborne bacteria by 16S rDNA sequencing, MALDI-TOF MS and the MIDI microbial identification system Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Identification of airborne bacteria by 16S rDNA sequencing, MALDI-TOF MS and the MIDI microbial identification system
Creator	Larsson, Anders Dybwad, Marius Fykse, Else Humppi, Tarmo Ingebretsen, Andre Olofsson, Göran Skogan, Gunnar Tjärnhage, Torbjörn Blatny, Martha Eggen, Sørvik Janet, • Per-Å Ke Gradmark, Wästerby Pär, • Vilde, •
source	PMC
abstract	The aim of this study was to collect and identify airborne bacteria in Norway, Sweden and Finland and to compare three different technologies for identifying collected airborne bacterial isolates: the “gold standard” method 16S rDNA sequencing, MALDI-TOF MS using the MALDI Biotyper 2.0 and the MIDI Sherlock(®) Microbial Identification System (MIDI MIS system). Airborne bacteria were collected during three different periods from May to October 2009 using air sampling directly on agar plates. A total of 140 isolates were collected during three sampling campaigns, and 74 % (103) of these isolates were analyzed by all three methods. The dominant genera in Norway and Finland were the gram-positive bacteria Bacillus and Staphylococcus, whereas the gram-negative bacterium Acinetobacter was the dominant genus in Sweden. Using 16S rDNA sequencing, MALDI-TOF MS and MIDI MIS analysis, 83, 79 and 75 %, respectively, of the isolates were identified and assigned to order or higher taxonomic levels. In this study, the MALDI-TOF MS combining with the MALDI Biotyper 2.0 classification tool was demonstrated to be a fast and reliable alternative for identifying the airborne bacterial isolates. These studies have increased knowledge about the airborne bacterial background in outdoor air, which can be useful for evaluating and improving the operational performance of biological detectors in various environments. To our knowledge, this is the first time that 16S rDNA sequencing, MALDI-TOF MS and MIDI MIS analysis technologies have been compared for their efficiency in identifying airborne bacteria.
has issue date	2015-01-17(xsd:dateTime)
bibo:doi	10.1007/s10453-015-9363-9
bibo:pmid	32214629
has license	no-cc
sha1sum (hex)	d0d1203ec39dcc1a0fdbe2239364eff578c56d0c
schema:url	https://doi.org/10.1007/s10453-015-9363-9
resource representing a document's title	Identification of airborne bacteria by 16S rDNA sequencing, MALDI-TOF MS and the MIDI microbial identification system
has PubMed Central identifier	PMC7087874
has PubMed identifier	32214629
schema:publication	Aerobiologia (Bologna)
resource representing a document's body	covid:d0d1203ec39dcc1a0fdbe2239364eff578c56d0c#body_text
is schema:about of	named entity '16S rDNA' named entity 'assigned' named entity 'taxonomic' named entity 'MIDI' named entity 'bacterial' named entity 'system' named entity 'dominant' named entity 'background' named entity 'performance' named entity 'total' named entity '2009' named entity 'MIS' named entity 'isolates' named entity 'efficiency' named entity 'Sweden' named entity 'compared' named entity 'These' named entity '16S rDNA' named entity 'MALDI-TOF MS' named entity 'MIDI' named entity 'agar plates' named entity 'MIS' named entity 'Bacillus' named entity 'gram-negative bacterium' named entity 'MALDI' named entity 'bacteria' named entity 'microbial' named entity 'MALDI-TOF MS' named entity 'Staphylococcus' named entity 'MALDI-TOF MS' named entity 'MALDI' named entity '16S rDNA' named entity 'MALDI-TOF MS' named entity 'taxonomic identification' named entity 'Germany' named entity 'Finland' named entity 'genus' named entity 'MIDI' named entity 'Norwegian Defence Research Establishment' named entity 'MIDI' named entity 'Tampere' named entity 'Mycobacterium tuberculosis' named entity 'Bacteria' named entity 'Darmstadt' named entity 'Norway' named entity '16S rDNA' named entity 'gram-positive' named entity 'cloned' named entity 'MicroFlex' named entity 'Operon' named entity 'coniferous forest' named entity 'Pseudomonas' named entity 'MIDI' named entity 'Bacillus' named entity 'BLASTn' named entity 'MIS' named entity 'Bacillus' named entity 'Proteobacteria' named entity '16S rDNA' named entity '16S rDNA' named entity 'Acinetobacter' named entity 'Norway' named entity 'MALDI-TOF MS' named entity 'gold standard' named entity 'taxonomic level' named entity 'Firmicutes' named entity 'PCR' named entity 'Microorganisms'

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