About: Porcine Hemagglutinating Encephalomyelitis Virus Enters Neuro-2a Cells via Clathrin-Mediated Endocytosis in a Rab5-, Cholesterol-, and pH-Dependent Manner

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Porcine Hemagglutinating Encephalomyelitis Virus Enters Neuro-2a Cells via Clathrin-Mediated Endocytosis in a Rab5-, Cholesterol-, and pH-Dependent Manner Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Porcine Hemagglutinating Encephalomyelitis Virus Enters Neuro-2a Cells via Clathrin-Mediated Endocytosis in a Rab5-, Cholesterol-, and pH-Dependent Manner
Creator	Zhang, Jing Gao, Feng He, Wenqi Lan, Yungang Lu, Huijun Song, Deguang Zhao, Kui Li, Zi Lv, Xiaoling Guan, Jiyu Hu, Shiyu Shi, Junchao Yang, Yawen Li, Citation Pfeiffer, Julie
Source	Medline; PMC
abstract	Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurovirulent coronavirus that invades the central nervous system (CNS) in piglets. Although important progress has been made toward understanding the biology of PHEV, many aspects of its life cycle remain obscure. Here we dissected the molecular mechanism underlying cellular entry and intracellular trafficking of PHEV in mouse neuroblastoma (Neuro-2a) cells. We first performed a thin-section transmission electron microscopy (TEM) assay to characterize the kinetics of PHEV, and we found that viral entry and transfer occur via membranous coating-mediated endo- and exocytosis. To verify the roles of distinct endocytic pathways, systematic approaches were used, including pharmacological inhibition, RNA interference, confocal microscopy analysis, use of fluorescently labeled virus particles, and overexpression of a dominant negative (DN) mutant. Quantification of infected cells showed that PHEV enters cells by clathrin-mediated endocytosis (CME) and that low pH, dynamin, cholesterol, and Eps15 are indispensably involved in this process. Intriguingly, PHEV invasion leads to rapid actin rearrangement, suggesting that the intactness and dynamics of the actin cytoskeleton are positively correlated with viral endocytosis. We next investigated the trafficking of internalized PHEV and found that Rab5- and Rab7-dependent pathways are required for the initiation of a productive infection. Furthermore, a GTPase activation assay suggested that endogenous Rab5 is activated by PHEV and is crucial for viral progression. Our findings demonstrate that PHEV hijacks the CME and endosomal system of the host to enter and traffic within neural cells, providing new insights into PHEV pathogenesis and guidance for antiviral drug design. IMPORTANCE Porcine hemagglutinating encephalomyelitis virus (PHEV), a nonsegmented, positive-sense, single-stranded RNA coronavirus, invades the central nervous system (CNS) and causes neurological dysfunction. Neural cells are its targets for viral progression. However, the detailed mechanism underlying PHEV entry and trafficking remains unknown. PHEV is the etiological agent of porcine hemagglutinating encephalomyelitis, which is an acute and highly contagious disease that causes numerous deaths in suckling piglets and enormous economic losses in China. Understanding the viral entry pathway will not only advance our knowledge of PHEV infection and pathogenesis but also open new approaches to the development of novel therapeutic strategies. Therefore, we employed systematic approaches to dissect the internalization and intracellular trafficking mechanism of PHEV in Neuro-2a cells. This is the first report to describe the process of PHEV entry into nerve cells via clathrin-mediated endocytosis in a dynamin-, cholesterol-, and pH-dependent manner that requires Rab5 and Rab7.
has issue date	2017-11-14(xsd:dateTime)
bibo:doi	10.1128/jvi.01083-17
bibo:pmid	28956766
has license	cc-by
sha1sum (hex)	d1ab379487b829f2a3d3f78f27a427f2dd87deda
schema:url	https://doi.org/10.1128/jvi.01083-17
resource representing a document's title	Porcine Hemagglutinating Encephalomyelitis Virus Enters Neuro-2a Cells via Clathrin-Mediated Endocytosis in a Rab5-, Cholesterol-, and pH-Dependent Manner
has PubMed Central identifier	PMC5686734
has PubMed identifier	28956766
schema:publication	J Virol
resource representing a document's body	covid:d1ab379487b829f2a3d3f78f27a427f2dd87deda#body_text
is schema:about of	named entity 'membranous' named entity 'highly' named entity 'detailed' named entity 'mechanism' named entity 'losses' named entity 'kinetics' named entity 'cells' named entity 'Journal' named entity 'clathrin-mediated endocytosis' named entity 'cholesterol' named entity 'infection' named entity 'mechanism' named entity 'central nervous system (CNS)' named entity 'clathrin-mediated endocytosis' named entity 'Journal' named entity 'viral' named entity 'Rab' named entity 'cellular' named entity 'acute' named entity 'Virus' named entity 'REMAINS' covid:arg/d1ab379487b829f2a3d3f78f27a427f2dd87deda named entity 'RAB5' named entity 'ACTIVATED' named entity 'DISSECTED' named entity 'CENTRAL NERVOUS SYSTEM' named entity 'ENDOCYTOSIS' named entity 'TO CHARACTERIZE' named entity 'CONFOCAL MICROSCOPY' named entity 'VIRAL' named entity 'DEATHS' named entity 'POSITIVE' named entity 'NOVEL' named entity 'RABS' named entity 'ASM' named entity 'CHOLESTEROL' named entity 'PROGRESS' named entity 'ORG' named entity 'TARGETS' named entity 'BUT' named entity 'INSIGHTS' named entity 'MEMBRANOUS' named entity 'DECEMBER' named entity 'DYNAMIN' named entity 'CORONAVIRUS' named entity 'MOLECULAR MECHANISM' named entity 'CAUSES' named entity 'REARRANGEMENT' named entity 'CLATHRIN-MEDIATED ENDOCYTOSIS' named entity 'INFECTION' named entity 'CELLS' named entity 'REQUIRED' named entity 'KNOWLEDGE' named entity 'QUANTIFICATION' named entity 'ASSAY' named entity 'INVASION' named entity 'ACTIN CYTOSKELETON' named entity 'EPS15' named entity 'DETAILED' named entity 'TRAFFICKING' named entity 'PHARMACOLOGICAL' named entity 'OPEN' named entity 'DRUG DESIGN' named entity 'INTACTNESS' named entity 'NEURO-2A' named entity 'HIGHLY CONTAGIOUS DISEASE' named entity 'RNA INTERFERENCE'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software