About: Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
Creator	Aurélio, Marco Durigon, Edison Sáfadi, Palazzi Thomazelli, Luciano De Paulis, Milena Fongaro Botosso, Viviane Luís, & Machado, Guaragna Rahal, Rafael Renato, João Bruna, Danielle Oliveira, Leal Almeida, Flavia Araujo, Danielle Chalup, Vanessa Dorlass, Erick Ferreira, Sabrina Fock, Ricardo Matos, Samantha Mello, Ralyria Soares, Camila Soledade, Mariana Souza, Eloisa Sucupira, Carolina Ferreira, Carlos Anita, Rubia Araujo Valério, Camila Barrientos, Mott Carlotta, Anna Célia, & Donizette Candido, Erika Fabyano, & Ferreira, Leticia Gregorio, Daniella Janaina, & Larotonda Telezynski, Bruna Leal, Bruno Martins Sodré, Joice Mello Malta, Fernanda Nunez Pinez, Miranda Oliveira Monteiro, Cairo Oliveira Viana, Amanda Oliveira, Cairo Palamin Buonafine, Carolina Pinho, Rebelo Prado, Bonfim Santana, Ferraz Simões Aguiar, Andressa
source	Medline; PMC
abstract	In March 2020, WHO declared a pandemic state due to SARS-CoV-2 having spread. TaqMan-based real-time RT-qPCR is currently the gold standard for COVID-19 diagnosis. However, it is a high-cost assay, inaccessible for the majority of laboratories around the world, making it difficult to diagnose on a large scale. The objective of this study was to standardize lower cost molecular methods for SARS-CoV-2 identification. E gene primers previously determined for TaqMan assays by Colman et al. (2020) were adapted in SYBR Green assay and RT-PCR conventional. The cross-reactivity test was performed with 17 positive samples for other respiratory viruses, and the sensibility test was performed with 8 dilutions (10 based) of SARS-CoV-2 isolated and 63 SARS-CoV-2-positive samples. The SYBR Green assays and conventional RT-PCR have not shown amplification of the 17 respiratory samples positives for other viruses. The SYBR Green-based assay was able to detect all 8 dilutions of the isolate. The conventional PCR detected until 10(7) dilution, both assays detected the majority of the 63 samples, 98.42% of positivity in SYBR Green, and 93% in conventional PCR. The average Ct variation between SYBR Green and TaqMan was 1.92 and the highest Ct detected by conventional PCR was 35.98. Both of the proposed assays are less sensitive than the current gold standard; however, our data shows a low sensibility variation, suggesting that these methods could be used by laboratories as a lower cost molecular method for SARS-CoV-2 diagnosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s42770-020-00347-5) contains supplementary material, which is available to authorized users.
has issue date	2020-08-07(xsd:dateTime)
bibo:doi	10.1007/s42770-020-00347-5
bibo:pmid	32767275
has license	no-cc
sha1sum (hex)	d32ba1d8102e33885145f63718abe1b3b0215e9c
schema:url	https://doi.org/10.1007/s42770-020-00347-5
resource representing a document's title	Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
has PubMed Central identifier	PMC7411266
has PubMed identifier	32767275
schema:publication	Braz J Microbiol
resource representing a document's body	covid:d32ba1d8102e33885145f63718abe1b3b0215e9c#body_text
is schema:about of	named entity 'METHODS' named entity 'RT-QPCR' named entity 'DETERMINED' named entity 'SARS-CoV-2' named entity 'WHO' named entity 'COVID-19' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'RT-PCR' named entity 'RT-PCR' named entity 'complementary DNA' named entity 'Applied Biosystems' named entity 'Applied Biosystems' named entity 'RT-PCR' named entity 'diagnostic methods' named entity 'DEPC' named entity 'RT-PCR' named entity 'WHO' named entity 'Germany' named entity 'diagnostic method' named entity 'SARS-CoV-2' named entity 'RT-PCR' named entity 'SYBR Green' named entity 'COVID-19' named entity 'ATCC' named entity 'virus' named entity 'USP' named entity 'SYBR Green' named entity 'SARS-CoV-2' named entity 'positive controls' named entity 'PCR' named entity 'São Paulo University' named entity 'SYBR Green' named entity 'São Luiz Gonzaga'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software