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About:
Intense circulation of A/H5N1 and other avian influenza viruses in Cambodian live-bird markets with serological evidence of sub-clinical human infections
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schema:ScholarlyArticle
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wasabi.inria.fr
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Type:
Academic Article
research paper
schema:ScholarlyArticle
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type
Academic Article
research paper
schema:ScholarlyArticle
isDefinedBy
Covid-on-the-Web dataset
title
Intense circulation of A/H5N1 and other avian influenza viruses in Cambodian live-bird markets with serological evidence of sub-clinical human infections
Creator
Buchy, Philippe
Rith, Sareth
Ly, Sowath
Tarantola, Arnaud
Kalpravidh, Wantanee
Holl, Davun
Dussart, Philippe
Horwood, Paul
Duong, Veasna
Allal, Lotfi
Gambaretti, Juliette
Sorn, San
Horm, Srey
Phalla, Y
source
Medline; PMC
abstract
Surveillance for avian influenza viruses (AIVs) in poultry and environmental samples was conducted in four live-bird markets in Cambodia from January through November 2013. Through real-time RT-PCR testing, AIVs were detected in 45% of 1048 samples collected throughout the year. Detection rates ranged from 32% and 18% in duck and chicken swabs, respectively, to 75% in carcass wash water samples. Influenza A/H5N1 virus was detected in 79% of samples positive for influenza A virus and 35% of all samples collected. Sequence analysis of full-length haemagglutinin (HA) and neuraminidase (NA) genes from A/H5N1 viruses, and full-genome analysis of six representative isolates, revealed that the clade 1.1.2 reassortant virus associated with Cambodian human cases during 2013 was the only A/H5N1 virus detected during the year. However, multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of HA and NA genes revealed co-circulation of at least nine low pathogenic AIVs from HA1, HA2, HA3, HA4, HA6, HA7, HA9, HA10 and HA11 subtypes. Four repeated serological surveys were conducted throughout the year in a cohort of 125 poultry workers. Serological testing found an overall prevalence of 4.5% and 1.8% for antibodies to A/H5N1 and A/H9N2, respectively. Seroconversion rates of 3.7 and 0.9 cases per 1000 person-months participation were detected for A/H5N1 and A/H9N2, respectively. Peak AIV circulation was associated with the Lunar New Year festival. Knowledge of periods of increased circulation of avian influenza in markets should inform intervention measures such as market cleaning and closures to reduce risk of human infections and emergence of novel AIVs.
has issue date
2016-07-20
(
xsd:dateTime
)
bibo:doi
10.1038/emi.2016.69
bibo:pmid
27436362
has license
cc-by
sha1sum (hex)
d3cbb775040a7d1eac36c6615653b2d94f6c6c13
schema:url
https://doi.org/10.1038/emi.2016.69
resource representing a document's title
Intense circulation of A/H5N1 and other avian influenza viruses in Cambodian live-bird markets with serological evidence of sub-clinical human infections
has PubMed Central identifier
PMC5141262
has PubMed identifier
27436362
schema:publication
Emerg Microbes Infect
resource representing a document's body
covid:d3cbb775040a7d1eac36c6615653b2d94f6c6c13#body_text
is
schema:about
of
named entity 'INFECTIONS'
named entity '2013'
named entity 'clade'
named entity 'subtypes'
named entity '2013'
named entity 'multiplex'
named entity 'antibodies'
named entity 'measures'
named entity 'avian influenza'
named entity 'LIVE'
named entity 'HUMAN'
covid:arg/d3cbb775040a7d1eac36c6615653b2d94f6c6c13
named entity 'MARKET'
named entity 'MULTIPLEX'
named entity 'HA6'
named entity 'HA2'
named entity 'INFECTIONS'
named entity 'MARKETS'
named entity 'REVEALED'
named entity 'SEROCONVERSION'
named entity 'HAEMAGGLUTININ'
named entity 'DETECTED'
named entity 'NEURAMINIDASE'
named entity 'NEW'
named entity 'RATES'
named entity 'FOUND'
named entity 'BIRD'
named entity 'REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION'
named entity 'HA3'
named entity 'INFLUENZA A'
named entity 'SWABS'
named entity 'HUMAN'
named entity 'HA7'
named entity 'INCREASED'
named entity 'WORKERS'
named entity '125'
named entity 'POULTRY'
named entity 'SEQUENCE ANALYSIS'
named entity 'VIRUSES'
named entity 'REPEATED'
named entity 'CLEANING'
named entity 'LUNAR'
named entity 'SEROLOGICAL'
named entity 'YEAR'
named entity 'CHICKEN'
named entity 'EMERGENCE'
named entity 'ASSOCIATED WITH'
named entity 'CASES'
named entity 'CIRCULATION'
named entity 'VIRUS ASSOCIATED'
named entity 'OVERALL'
named entity 'TESTING'
named entity 'HA1'
named entity 'PREVALENCE'
named entity 'PEAK'
named entity 'PCR'
named entity 'PARTICIPATION'
named entity '4.5'
named entity 'INFLUENZA VIRUSES'
named entity 'INFLUENZA A VIRUS'
named entity 'MEASURES'
named entity 'REAL-TIME RT-PCR'
named entity 'NOVEL'
named entity 'LENGTH'
named entity 'ISOLATES'
named entity 'KNOWLEDGE'
named entity 'REPRESENTATIVE'
named entity '1000'
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