About: Selection and validation of suitable reference genes for qPCR gene expression analysis in goats and sheep under Peste des petits ruminants virus (PPRV), lineage IV infection

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About: Selection and validation of suitable reference genes for qPCR gene expression analysis in goats and sheep under Peste des petits ruminants virus (PPRV), lineage IV infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Selection and validation of suitable reference genes for qPCR gene expression analysis in goats and sheep under Peste des petits ruminants virus (PPRV), lineage IV infection
Creator	Mishra, Bina Pandey, Aruna Saxena, Shikha Chaudhary, Dheeraj Khanduri, Alok Mondal, Piyali Singh, Raj Gandham, Ravi Khan, Ishaq Malla, Waseem Mishra, Bishnu Muthuchelvan, D Rajak, Kaushal Ranjan, Amit Sahoo, Aditya Tiwari, Ashok Wani, Sajad
Source	Medline; PMC
abstract	Identification of suitable candidate reference genes is an important prerequisite for validating the gene expression data obtained from downstream analysis of RNA sequencing using quantitative real time PCR (qRT-PCR). Though existence of a universal reference gene is myth, commonly used reference genes can be assessed for expression stability to confer their suitability to be used as candidate reference genes in gene expression studies. In this study, we evaluated the expression stability of ten most commonly used reference genes (GAPDH, ACTB, HSP90, HMBS, 18S rRNA, B2M, POLR2A, HPRT1, ACAC, YWHAZ) in fourteen different Peste des petits ruminants virus (PPRV) infected tissues of goats and sheep. RefFinder and RankAggreg software were used to deduce comprehensive ranking of reference genes. Our results suggested HMBS and B2M in goats and HMBS and HPRT1 in sheep can be used as suitable endogenous controls in gene expression studies of PPRV infection irrespective of tissues and condition as a whole, thus eliminating the use of tissue specific/ condition specific endogenous controls. We report for the first time suitable reference genes for gene expression studies in PPRV infected tissues. The reference genes determined here can be useful for future studies on gene expression in sheep and goat infected with PPRV, thus saving extra efforts and time of repeating the reference gene determination and validation.
has issue date	2018-10-29(xsd:dateTime)
bibo:doi	10.1038/s41598-018-34236-7
bibo:pmid	30374051
has license	cc-by
sha1sum (hex)	d4090dff0fba51422afb6bf34f29c10f9a35caac
schema:url	https://doi.org/10.1038/s41598-018-34236-7
resource representing a document's title	Selection and validation of suitable reference genes for qPCR gene expression analysis in goats and sheep under Peste des petits ruminants virus (PPRV), lineage IV infection
has PubMed Central identifier	PMC6206032
has PubMed identifier	30374051
schema:publication	Sci Rep
resource representing a document's body	covid:d4090dff0fba51422afb6bf34f29c10f9a35caac#body_text
is schema:about of	named entity 'LINEAGE' named entity 'VALIDATION' named entity 'genes' named entity 'ACAC' named entity 'gene' named entity 'expression' named entity '18S rRNA' named entity 'controls' named entity 'GAPDH' named entity 'infected' named entity 'genes' named entity 'studies' named entity 'virus' named entity 'expression' named entity 'OPEN' named entity 'INFECTION' named entity 'CONDITION' named entity 'FUTURE' named entity 'ACAC' named entity 'DETERMINATION' named entity 'IMPORTANT' named entity 'TIME' named entity 'OBTAINED' named entity 'USING' named entity 'GOATS' named entity 'USED' named entity 'RNA SEQUENCING' named entity 'STUDIES' named entity 'REFERENCE GENE' named entity 'INFECTION' named entity 'INFECTED' named entity 'GENE EXPRESSION' named entity 'ACTB' named entity 'VALIDATION' named entity 'ASSESSED' named entity 'STABILITY' named entity 'REPEATING' named entity 'AND CONDITION' named entity 'EXTRA' named entity 'EXPRESSION' named entity 'CANDIDATE' named entity 'SPECIFIC' named entity 'YWHAZ' named entity 'COMPREHENSIVE' named entity 'TISSUES' named entity 'SUITABLE' named entity 'STUDY' named entity 'GENES' named entity '18S RRNA' named entity 'SUITABLE' named entity 'IDENTIFICATION' named entity 'SHEEP' named entity 'GOATS' named entity 'GENE EXPRESSION ANALYSIS' named entity 'OPEN' named entity 'PESTE DES PETITS RUMINANTS VIRUS' named entity 'QPCR' named entity 'DETERMINED' named entity 'PESTE DES PETITS RUMINANTS VIRUS' named entity 'MYTH' named entity 'REPORT' named entity 'TISSUE SPECIFIC' named entity 'GOAT' named entity 'OUR' named entity 'EVALUATED'

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