About: Adapted HCV JFH1 variant is capable of accommodating a large foreign gene insert and allows lower level HCV replication and viral production

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About: Adapted HCV JFH1 variant is capable of accommodating a large foreign gene insert and allows lower level HCV replication and viral production Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Adapted HCV JFH1 variant is capable of accommodating a large foreign gene insert and allows lower level HCV replication and viral production
Creator	Wang, Qi Liu, Shuanghu Hagedorn, Curt
source	PMC
abstract	Infectious HCV carrying reporter genes have further applications in understanding the HCV life cycle including replication, viral assembly and release. In this study, a full-length 3039bp LacZ gene was inserted into the derivative of JFH1-AM120 to develop an additional reporter virus. The results showed that the recombinant reporter virus JFH1-AM120-LacZ can replicate and produce lower titers of infectious virus. However, insertion of the LacZ gene in the C-terminal region of the NS5A in HCV JFH1-AM120-LacZ decreased viral replication and dramatically impaired the production of infectious viral particles. Moreover, the JFH1-AM120-LacZ reporter virus lost the LacZ gene after serial passage. Nevertheless, the JFH1-AM120-LacZ reporter virus displayed the entire life cycle of HCV, from replication to production of infectious virus, in Huh7.5 cells. This study demonstrates that the NS5A region of HCV JFH1-AM120 has the capacity to accommodate large foreign genes up to 3,039 bp and suggests that other relatively large gene inserts can be accommodated at this site.
has issue date	2018-07-13(xsd:dateTime)
bibo:doi	10.7150/ijbs.27411
bibo:pmid	30123070
has license	cc-by-nc
sha1sum (hex)	d73e2d3dfc88a88345c521192e8c38f8c69a132e
schema:url	https://doi.org/10.7150/ijbs.27411
resource representing a document's title	Adapted HCV JFH1 variant is capable of accommodating a large foreign gene insert and allows lower level HCV replication and viral production
has PubMed Central identifier	PMC6097470
has PubMed identifier	30123070
schema:publication	Int J Biol Sci
resource representing a document's body	covid:d73e2d3dfc88a88345c521192e8c38f8c69a132e#body_text
is schema:about of	named entity 'virus' named entity 'virus' named entity 'large' named entity 'HCV' named entity 'virus' named entity 'develop' named entity 'HCV' named entity 'cells' named entity 'LENGTH' named entity 'INSERTED INTO' named entity 'GENE' named entity 'VIRAL ASSEMBLY' named entity 'study' named entity 'gene' named entity 'HCV' named entity 'virus' named entity 'release' named entity 'LacZ' named entity 'assembly' named entity 'LacZ' named entity 'virus' named entity 'gene' named entity 'viral assembly' named entity 'virus' named entity 'life cycle' named entity 'gene' named entity 'HCV' named entity 'LacZ' named entity 'codon' named entity 'Promega' named entity 'Nikon' named entity 'gene' named entity 'milliliter' named entity 'C-terminal region' named entity 'C-terminus' named entity 'LacZ' named entity 'EGFP' named entity 'gene' named entity 'β-galactosidase' named entity 'HCV' named entity 'NS5A' named entity 'trypsinized' named entity 'substrate' named entity 'Austin, TX' named entity 'DMEM' named entity 'vector' named entity 'adaptive mutation' named entity 'NS5A' named entity 'gene' named entity 'Bio-Rad' named entity 'titer' named entity 'protein' named entity 'gene' named entity 'LacZ' named entity 'protein' named entity 'Invitrogen' named entity 'LacZ' named entity 'X-Gal' named entity 'staining' named entity 'HCV' named entity 'PBS' named entity 'HCV' named entity 'staining' named entity 'LacZ' named entity 'transfected' named entity 'serially diluted' named entity 'RNA' named entity 'NS5A' named entity 'staining' named entity 'virus' named entity 'PCR' named entity 'cirrhosis' named entity 'amino acids' named entity 'gene' named entity 'HCV' named entity 'blue color' named entity '1, 2' named entity 'antivirals' named entity 'Huh7'

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