About: Susceptibility of porcine IPEC-J2 intestinal epithelial cells to infection with porcine deltacoronavirus (PDCoV) and serum cytokine responses of gnotobiotic pigs to acute infection with IPEC-J2 cell culture-passaged PDCoV

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About: Susceptibility of porcine IPEC-J2 intestinal epithelial cells to infection with porcine deltacoronavirus (PDCoV) and serum cytokine responses of gnotobiotic pigs to acute infection with IPEC-J2 cell culture-passaged PDCoV Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Susceptibility of porcine IPEC-J2 intestinal epithelial cells to infection with porcine deltacoronavirus (PDCoV) and serum cytokine responses of gnotobiotic pigs to acute infection with IPEC-J2 cell culture-passaged PDCoV
Creator	Jung, Kwonil Hu, Hui Miyazaki, Ayako Saif, Linda
Source	Elsevier; Medline; PMC
abstract	The porcine small intestinal epithelial cell line, IPEC-J2, is useful to characterize the interactions of enterocytes with enteric viruses in vitro. We investigated whether IPEC-J2 cells are susceptible to porcine deltacoronavirus (PDCoV) infection. We conducted quantification of infectious virus or viral RNA, immunofluorescent (IF) staining for the detection of PDCoV antigens, and TUNEL assay in IPEC-J2 cells inoculated with the strain OH-FD22-P8 grown in LLC-PK cells, and supplemented with 10 μg/ml of trypsin in the cell culture medium. Cytopathic effects (CPE) that consisted of enlarged and rounded cells followed by cell shrinkage and detachment, were identified by the 3rd viral passage in the IPEC-J2 cells. PDCoV antigen was detected in the cells showing CPE. By double IF and TUNEL staining, most PDCoV antigen-positive IPEC-J2 cells failed to show TUNEL-positive signals, indicating that PDCoV-infected IPEC-J2 cells may not undergo apoptosis, but rather necrosis, similar to necrotic cell death of infected enterocytes in vivo. There was increased interleukin-6 in PDCoV-infected IPEC-J2 cell culture supernatants at post-inoculation hour (PIH) 48–96, as evaluated by ELISA, concurrent with increased titers of PDCoV at PIH 24–72. The susceptibility of IPEC-J2 cells to PDCoV infection supports their usefulness to characterize the interactions of enterocytes with PDCoV. We also demonstrated that IPEC-J2 cell culture-passaged PDCoV (OH-FD22-P8-I-P4) was enteropathogenic in 10-day-old gnotobiotic pigs, and induced systemic innate and pro-inflammatory cytokine responses during the acute PDCoV infection.
has issue date	2018-05-30(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2018.05.019
bibo:pmid	29981708
has license	no-cc
sha1sum (hex)	d894d456e2457014fd72331b48c42be3f6d263c5
schema:url	https://doi.org/10.1016/j.vetmic.2018.05.019
resource representing a document's title	Susceptibility of porcine IPEC-J2 intestinal epithelial cells to infection with porcine deltacoronavirus (PDCoV) and serum cytokine responses of gnotobiotic pigs to acute infection with IPEC-J2 cell culture-passaged PDCoV
has PubMed Central identifier	PMC7117386
has PubMed identifier	29981708
schema:publication	Vet Microbiol
resource representing a document's body	covid:d894d456e2457014fd72331b48c42be3f6d263c5#body_text
is schema:about of	named entity 'staining' named entity 'infected' named entity 'PIH' named entity 'detected' named entity 'supports' named entity 'cytokine' named entity 'ACUTE INFECTION' named entity 'CYTOKINE ' named entity 'THEIR' named entity 'PIGS' named entity 'CELL' named entity 'SHRINKAGE' named entity 'ACUTE' named entity 'INCREASED' named entity 'STAINING' named entity 'APOPTOSIS' named entity 'PORCINE DELTACORONAVIRUS' named entity 'HOUR' named entity 'STRAIN' named entity 'INFECTION' named entity 'SYSTEMIC' named entity 'ENTEROPATHOGENIC' named entity 'USEFUL' named entity 'POSITIVE' named entity 'TRYPSIN' named entity 'CELL CULTURE' named entity 'VIRUSES' named entity 'DETECTED' named entity 'FAILED' named entity 'VIRAL' named entity 'IN VITRO' named entity 'PORCINE' named entity 'SUSCEPTIBLE' named entity 'CPE' named entity 'INTESTINAL' named entity 'INDUCED' named entity 'ANTIGEN' named entity 'QUANTIFICATION' named entity 'PRO-INFLAMMATORY CYTOKINE' named entity 'NECROSIS' named entity 'NECROTIC CELL DEATH' named entity 'SERUM CYTOKINE' named entity 'PORCINE' named entity 'TO CHARACTERIZE' named entity 'IDENTIFIED BY' named entity 'SIMILAR' named entity 'ROUNDED' named entity 'CONCURRENT WITH' named entity 'ELISA' named entity 'CYTOPATHIC EFFECTS' named entity 'CULTURE MEDIUM' named entity 'EVALUATED' named entity 'RATHER' named entity 'LLC' named entity 'DETACHMENT' named entity 'CELLS' named entity 'INDICATING' named entity 'DAY-OLD' named entity 'EPITHELIAL CELL LINE' named entity 'OH-' named entity 'SMALL INTESTINAL EPITHELIAL CELL' named entity 'DOUBLE' named entity 'PORCINE DELTACORONAVIRUS' named entity 'RESPONSES' named entity 'PIGS' named entity 'ANTIGENS' named entity 'INFECTION' named entity 'SIGNALS' named entity 'RESPONSES' named entity 'IN VIVO' named entity 'FOLLOWED BY' named entity 'ENLARGED' named entity 'PIH' named entity 'VIRAL RNA' named entity 'SUSCEPTIBILITY' named entity 'PASSAGE' named entity 'ENTEROCYTES' named entity 'TUNEL ASSAY'

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