About: A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus

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About: A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus
Creator	Weidmann, Manfred Niedrig, Matthias Sakuntabhai, Anavaj Thaloengsok, Sasikanya Kaiser, Marco Leparc-Goffart, Isabelle Prüger, Pauline Ubol, Sukathida Hufert, Frank Sall, Amadou El, Wahed Faye, Pranav, Patel
source	PMC
abstract	BACKGROUND: Chikungunya virus (CHIKV) is a mosquito-borne virus currently transmitted in about 60 countries. CHIKV causes acute flu-like symptoms and in many cases prolonged musculoskeletal and joint pain. Detection of the infection is mostly done using RT-RCR or ELISA, which are not suitable for point-of-care diagnosis. METHODOLOGY/PRINCIPAL FINDINGS: In this study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of the CHIKV was developed. The assay sensitivity, specificity, and cross-reactivity were tested. CHIKV RT-RPA assay detected down to 80 genome copies/reaction in a maximum of 15 minutes. It successfully identified 18 isolates representing the three CHIKV genotypes. No cross-reactivity was detected to other alphaviruses and arboviruses except O'nyong'nyong virus, which could be differentiated by a modified RPA primer pair. Seventy-eight samples were screened both by RT-RPA and real-time RT-PCR. The diagnostic sensitivity and specificity of the CHIKV RT-RPA assay were determined at 100%. CONCLUSIONS/SIGNIFICANCE: The developed RT-RPA assay represents a promising method for the molecular detection of CHIKV at point of need.
has issue date	2016-09-29(xsd:dateTime)
bibo:doi	10.1371/journal.pntd.0004953
bibo:pmid	27685649
has license	cc-by
sha1sum (hex)	da215867201d36fa409a1f64aa89dfe75d537784
schema:url	https://doi.org/10.1371/journal.pntd.0004953
resource representing a document's title	A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus
has PubMed Central identifier	PMC5042537
has PubMed identifier	27685649
schema:publication	PLoS Negl Trop Dis
resource representing a document's body	covid:da215867201d36fa409a1f64aa89dfe75d537784#body_text
is schema:about of	named entity 'flu-like symptoms' named entity 'CHIKV' named entity 'Rapid' named entity 'Detection' named entity 'ACUTE' named entity 'ELISA' named entity 'JOINT PAIN' named entity 'CASES' named entity 'DIAGNOSIS' named entity 'IS A' named entity 'VIRUS' named entity 'RCR' named entity 'SUITABLE' named entity 'POINT-OF-CARE' named entity 'CHIKUNGUNYA VIRUS' named entity 'FIELD' named entity 'CAUSES' named entity 'ABOUT' named entity 'USING' named entity 'COUNTRIES' named entity 'RAPID' named entity 'POLYMERASE' named entity 'AMPLIFICATION' named entity 'REVERSE TRANSCRIPTION' named entity 'ASSAY' named entity 'BORNE' named entity 'MOSQUITO' named entity 'FLU-LIKE SYMPTOMS' named entity 'CURRENTLY' named entity 'INFECTION' named entity 'DETECTION' named entity 'PROLONGED' named entity 'TRANSMITTED' named entity 'MUSCULOSKELETAL' named entity 'DETECTION' named entity 'RECOMBINASE' named entity 'CHIKUNGUNYA VIRUS' covid:arg/da215867201d36fa409a1f64aa89dfe75d537784 named entity 'musculoskeletal' named entity 'cases' named entity 'Detection' named entity 'CHIKV' named entity 'CHIKV' named entity 'virus' named entity 'flu-like symptoms' named entity 'Assay' named entity 'Chikungunya Virus' named entity 'cross reactivity' named entity 'pathogens' named entity 'infection' named entity 'real-time PCR' named entity 'PDF' named entity 'Africa' named entity 'CHIKV' named entity 'CHIKV' named entity 'RPA' named entity 'CHIKV' named entity 'RPA' named entity 'ambient temperature' named entity 'CHIKV' named entity 'real-time RT-PCR' named entity 'Americas' named entity 'CHIKV' named entity 'reverse transcription' named entity 'CHIKV' named entity 'fluorophore' named entity 'primers' named entity 'endemic' named entity 'ONNV'

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