About: Molecular detection of Enteropathogens from diarrheic stool of HIV positive patients in Gondar, Ethiopia

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About: Molecular detection of Enteropathogens from diarrheic stool of HIV positive patients in Gondar, Ethiopia Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular detection of Enteropathogens from diarrheic stool of HIV positive patients in Gondar, Ethiopia
Creator	Abere, Aberham Getie, Sisay Seid, Lubaba Stokes, William Tesfa, Habtie Pillai, Dylan Bayih, Abebe
Source	Medline; PMC
abstract	BACKGROUND: Infectious diarrhea is a common problem in the developing world, especially among people living with HIV/AIDS. Traditional diagnostic methods such as stool culture and microscopic examination are limited by resources and poor sensitivity. The use of molecular diagnostics for enteropathogen detection in this region of sub-Saharan Africa has not been fully explored. We sought to identify risk factors and characterize enteropathogens from diarrheic stools of HIV-positive patients in Gondar, Ethiopia using multiplex molecular panels targeting key infectious agents. METHODS: A cross-sectional study of 100 stool samples was performed. Samples were collected consecutively from HIV- positive patients presenting with diarrhea at University of Gondar Hospital clinic, a major center in NW Ethiopia. Genomic DNA was extracted from stool and processed using a multiplex molecular panel Allplex™ [Seegene, Canada]. Correlations between patient characteristics, symptoms, public health risk factors, and enteropathogen type (s) were studied. Eighty-six samples were successfully analyzed by molecular methods. RESULTS: The mean age was 35 with 43% male. Eighty percent lived in an urban area, 18% had access to well water only, and 81% practiced proper hand hygiene. The majority of patients (72%) were receiving HAART with a median CD4 cell count of 362/μL. Multiple pathogens were detected in 94% of specimens, with an average of 5 enteropathogens per sample. Common bacteria, viruses, and parasites detected were Shigella spp./enteroinvasive E. coli (80%), enterotoxigenic E. coli (73%), Norovirus (16%) and B. hominis (62%). CD4 cell count < 500/ μL was associated with the presence of viruses (p = 0.004) and the absence of STEC (p = 0.010). The use of HAART or CD4 levels was not associated with the number of enteropathogens detected. CONCLUSIONS: Diarrheic stool from HIV-positive outpatients in Gondar, Ethiopia had on average 5 enteropathogens present in their stool. Shigellaspp./enteroinvasive E. coli and enterotoxigenic E. coli are the major pathogens, not dissimilar to immunocompetent individuals in low income countries.
has issue date	2018-07-31(xsd:dateTime)
bibo:doi	10.1186/s12879-018-3265-8
bibo:pmid	30064366
has license	cc-by
sha1sum (hex)	e31625f324f8a44bca6c701f2c6e6d7c09a0830c
schema:url	https://doi.org/10.1186/s12879-018-3265-8
resource representing a document's title	Molecular detection of Enteropathogens from diarrheic stool of HIV positive patients in Gondar, Ethiopia
has PubMed Central identifier	PMC6069753
has PubMed identifier	30064366
schema:publication	BMC Infect Dis
resource representing a document's body	covid:e31625f324f8a44bca6c701f2c6e6d7c09a0830c#body_text
is schema:about of	named entity 'outpatients' named entity 'microscopic' named entity 'common' named entity 'targeting' named entity 'Infectious diarrhea' named entity 'patients' named entity 'public health' named entity 'Canada' named entity 'immunocompetent individuals' named entity 'positive' named entity 'WATER' named entity '2880' named entity 'SYMPTOMS' named entity 'MULTIPLEX' named entity 'KEY' named entity 'INFECTIOUS DIARRHEA' named entity 'MEDIAN' named entity 'BACKGROUND' named entity 'LEVELS' named entity 'PRESENTING' named entity 'URBAN AREA' named entity 'INDIVIDUALS' named entity 'AGE' named entity 'HOMINIS' named entity 'POSITIVE' named entity 'SAMPLE' named entity 'PARASITES' named entity 'HOSPITAL CLINIC' named entity 'BCL' named entity 'PANEL' named entity 'MOLECULAR DIAGNOSTICS' named entity 'PERFORMED' named entity 'ASSOCIATED WITH' named entity '500' named entity 'IMMUNOCOMPETENT' named entity 'A MAJOR' named entity '004' named entity 'IS A' named entity 'PATIENT CHARACTERISTICS' named entity 'PRESENT' named entity 'STOOL CULTURE' named entity 'COLLECTED' named entity 'GENOMIC DNA' named entity '100' named entity 'RECEIVING' named entity 'HEALTH RISK' named entity 'NOROVIRUS' named entity 'OUTPATIENTS' named entity 'HAART' named entity 'GONDAR' named entity 'STOOL' named entity 'WORLD' named entity 'FULLY' named entity 'PROCESSED' named entity 'ACCESS' named entity 'MOLECULAR' named entity 'CENTER' named entity 'MEAN' named entity 'PRESENCE OF' named entity 'ABSENCE OF' named entity 'DIAGNOSTIC' named entity '28P' named entity 'MAJORITY' named entity 'THEIR' named entity 'PROPER HAND' named entity 'RESOURCES' named entity 'UNIVERSITY' named entity 'A COMMON' named entity 'NUMBER OF' named entity 'HIV POSITIVE' named entity 'SPP' named entity 'EXTRACTED' named entity 'CD4 CELL COUNT' named entity 'PEOPLE' named entity 'SPECIMENS'

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