About: Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface

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About: Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface
Creator	Nauwynck, Hans Xie, Jiexiong Theuns, Sebastiaan Cui, Tingting
Source	Medline; PMC
abstract	Intestinal epithelium functions as a barrier to protect multicellular organisms from the outside world. It consists of epithelial cells closely connected by intercellular junctions, selective gates which control paracellular diffusion of solutes, ions and macromolecules across the epithelium and keep out pathogens. Rotavirus is one of the major enteric viruses causing severe diarrhea in humans and animals. It specifically infects the enterocytes on villi of small intestines. The polarity of rotavirus replication in their target enterocytes and the role of intestinal epithelial integrity were examined in the present study. Treatment with EGTA, a drug that chelates calcium and disrupts the intercellular junctions, (i) significantly enhanced the infection of rotavirus in primary enterocytes, (ii) increased the binding of rotavirus to enterocytes, but (iii) considerably blocked internalization of rotavirus. After internalization, rotavirus was resistant to EGTA treatment. To investigate the polarity of rotavirus infection, the primary enterocytes were cultured in a transwell system and infected with rotavirus at either the apical or the basolateral surface. Rotavirus preferentially infected enterocytes at the basolateral surface. Restriction of infection through apical inoculation was overcome by EGTA treatment. Overall, our findings demonstrate that integrity of the intestinal epithelium is crucial in the host’s innate defense against rotavirus infection. In addition, the intercellular receptor is located basolaterally and disruption of intercellular junctions facilitates the binding of rotavirus to their receptor at the basolateral surface.
has issue date	2019-12-19(xsd:dateTime)
bibo:doi	10.1186/s13567-019-0728-x
bibo:pmid	31856906
has license	cc-by
sha1sum (hex)	e8b4609286a05e24738b9a4bb5f9b31091fc17fe
schema:url	https://doi.org/10.1186/s13567-019-0728-x
resource representing a document's title	Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface
has PubMed Central identifier	PMC6924034
has PubMed identifier	31856906
schema:publication	Vet Res
resource representing a document's body	covid:e8b4609286a05e24738b9a4bb5f9b31091fc17fe#body_text
is schema:about of	named entity 'chelates' named entity 'receptor' named entity 'epithelial cells' named entity 'After' named entity 'enhanced' named entity 'The' named entity 'host' named entity 'polarity' named entity 'Restriction' named entity 'diarrhea' named entity 'Porcine' named entity 'porcine' named entity 'DIFFUSION' named entity 'ROLE' named entity 'PRESENT' named entity 'INFECTION' named entity 'primary' named entity 'Intestinal epithelium' named entity 'primary' named entity 'animals' named entity 'severe' named entity 'enterocytes' named entity 'enterocytes' named entity 'infection' named entity 'Overall' named entity 'diffusion' named entity 'receptor' named entity 'resistant' named entity 'rotavirus' named entity 'infects' named entity 'Intestinal epithelium' named entity 'receptor' named entity 'infection' named entity 'enterocytes' named entity 'paracellular' named entity 'enterocytes' named entity 'intestinal epithelium' named entity 'small intestines' named entity 'rotavirus' named entity 'rotavirus' named entity 'macromolecules' named entity 'enterocytes' named entity 'Porcine' named entity 'Treatment' named entity 'enterocytes' named entity 'intercellular junctions' named entity 'EGTA' named entity 'inoculation' named entity 'DMEM' named entity 'staining' named entity 'sialidase' named entity 'paraformaldehyde' named entity 'inoculation' named entity 'supernatant' named entity 'neuraminidase' named entity 'enterocytes' named entity 'JAM' named entity 'analysis of variance' named entity 'cellular receptor' named entity 'enterocytes' named entity 'fluorescence microscopy' named entity 'respiratory syncytial virus' named entity 'VP6' named entity 'porcine' named entity 'adhesion molecules' named entity 'vacuolization' named entity 'hpi' named entity 'rotavirus' named entity 'zonula occludens' named entity 'enterocytes' named entity 'EGTA' named entity 'gene' named entity 'infection' named entity 'porcine' named entity 'enterocytes' named entity 'PBS' named entity 'luminal' named entity 'MOI'

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