About: Insertional protein engineering for analytical molecular sensing

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Insertional protein engineering for analytical molecular sensing Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Insertional protein engineering for analytical molecular sensing
Creator	Arís, Anna Vera, Andrea Villaverde, Antonio Ferraz, Rosa
Source	Medline; PMC
abstract	The quantitative detection of low analyte concentrations in complex samples is becoming an urgent need in biomedical, food and environmental fields. Biosensors, being hybrid devices composed by a biological receptor and a signal transducer, represent valuable alternatives to non biological analytical instruments because of the high specificity of the biomolecular recognition. The vast range of existing protein ligands enable those macromolecules to be used as efficient receptors to cover a diversity of applications. In addition, appropriate protein engineering approaches enable further improvement of the receptor functioning such as enhancing affinity or specificity in the ligand binding. Recently, several protein-only sensors are being developed, in which either both the receptor and signal transducer are parts of the same protein, or that use the whole cell where the protein is produced as transducer. In both cases, as no further chemical coupling is required, the production process is very convenient. However, protein platforms, being rather rigid, restrict the proper signal transduction that necessarily occurs through ligand-induced conformational changes. In this context, insertional protein engineering offers the possibility to develop new devices, efficiently responding to ligand interaction by dramatic conformational changes, in which the specificity and magnitude of the sensing response can be adjusted up to a convenient level for specific analyte species. In this report we will discuss the major engineering approaches taken for the designing of such instruments as well as the relevant examples of resulting protein-only biosensors.
has issue date	2006-04-03(xsd:dateTime)
bibo:doi	10.1186/1475-2859-5-15
bibo:pmid	16584558
has license	cc-by
sha1sum (hex)	e8d81518d127913ce42b22fbdd96d416c4f82e44
schema:url	https://doi.org/10.1186/1475-2859-5-15
resource representing a document's title	Insertional protein engineering for analytical molecular sensing
has PubMed Central identifier	PMC1459189
has PubMed identifier	16584558
schema:publication	Microb Cell Fact
resource representing a document's body	covid:e8d81518d127913ce42b22fbdd96d416c4f82e44#body_text
is schema:about of	named entity 'functioning' named entity 'proper' named entity 'biological' named entity 'protein engineering' named entity 'receptor' named entity 'parts' named entity 'ligand' named entity 'interaction' named entity 'complex' named entity 'affinity' named entity 'SPECIES' named entity 'RIGID' named entity 'MACROMOLECULES' named entity 'RATHER' named entity 'ENABLE' named entity 'USE' named entity 'RECOGNITION' named entity 'RESULTING' named entity 'LIGANDS' named entity 'MAJOR' named entity 'DEVICES' named entity 'QUANTITATIVE' named entity 'URGENT' named entity 'DIVERSITY' named entity 'BEING' named entity 'ANALYTE' named entity 'CONVENIENT' named entity 'NEW' named entity 'AFFINITY' named entity 'BIOMEDICAL' named entity 'INTERACTION' named entity 'SENSING' named entity 'CONTEXT' named entity 'LIGAND BINDING' named entity 'SENSORS' named entity 'INSTRUMENTS' named entity 'SENSING' named entity 'INDUCED' named entity 'CELL' named entity 'CONCENTRATIONS' named entity 'ENHANCING' named entity 'EFFICIENT' named entity 'CHANGES' named entity 'BIOLOGICAL' named entity 'ANALYTICAL' named entity 'PROPER' named entity 'USED' named entity 'WHERE' named entity 'SIGNAL TRANSDUCTION' named entity 'INSERTIONAL' named entity 'EITHER' named entity 'RECEPTOR' named entity 'TAKEN' named entity 'PROTEIN ' named entity 'LEVEL' named entity 'RESPONSE' named entity 'TRANSDUCER' named entity 'HIGH' named entity 'UP TO' named entity 'COMPLEX' named entity 'CASES' named entity 'RECENTLY' named entity 'RANGE' named entity 'PARTS' named entity 'REPRESENT' named entity 'IMPROVEMENT' named entity 'PRODUCTION' named entity 'SIGNAL TRANSDUCER' named entity 'HYBRID' named entity 'FOOD' named entity 'PROCESS' named entity 'SPECIFICITY' named entity 'LIGAND' named entity 'CHEMICAL' covid:arg/e8d81518d127913ce42b22fbdd96d416c4f82e44 named entity 'NON' named entity 'ENVIRONMENTAL' named entity 'ADJUSTED'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software