About: Intracranial Inoculation Is More Potent Than Intranasal Inoculation for Inducing Optic Neuritis in the Mouse Hepatitis Virus-Induced Model of Multiple Sclerosis

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About: Intracranial Inoculation Is More Potent Than Intranasal Inoculation for Inducing Optic Neuritis in the Mouse Hepatitis Virus-Induced Model of Multiple Sclerosis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Intracranial Inoculation Is More Potent Than Intranasal Inoculation for Inducing Optic Neuritis in the Mouse Hepatitis Virus-Induced Model of Multiple Sclerosis
Creator	Das Sarma, Jayasri Dine, Kimberly Singh, Manmeet Liu, Wenjun Khan, Reas Shindler, Kenneth Zeng, Jianxiong
Source	PMC
abstract	Neurotropic strains of mouse hepatitis virus (MHV) induce acute inflammation and chronic demyelination in the spinal cord and optic nerves mediated by axonal spread following intracranial inoculation in mice, with pathologic features similar to the human demyelinating disease multiple sclerosis. Spinal cord demyelination is also induced following intranasal inoculation with neurotropic MHV strains, however much higher viral doses are required as compared to intracranial inoculation. Recently, it was shown that intranasal administration of low concentrations of proteins leads to significant, rapid accumulation of protein in the optic nerve and in the eye, with only low levels reaching spinal cord and other brain regions. Thus, we examined whether intranasal inoculation with MHV at doses equivalent to those given intracranially could induce optic neuritis—inflammation, demyelination and loss of retinal ganglion cells (RGCs) in the optic nerve with or without inducing spinal cord demyelination. Four week old male C57BL/6J mice were inoculated intracranially with the recombinant demyelinating strain RSA59, or intranasally with RSA59 or the non-demyelinating strain RSMHV2 as control. One month post-inoculation, mice inoculated intracranially with RSA59 had significant myelin loss in both spinal cord and optic nerves, with significant loss of RGCs as well, consistent with prior studies. As expected, intranasal inoculation with RSA59 failed to induce demyelination in spinal cord; however, it also did not induce optic nerve demyelination. No acute inflammation was found, and no viral antigen was detected, in the optic nerve or retina 1 day after inoculation. Results confirm the neurotropic effects of RSA59 following intracranial inoculation, and suggest that direct infection with axonal transport of virus from brain to spinal cord and optic nerve is required to induce demyelinating disease. These studies suggest that MHV does not selectively concentrate in optic nerve and retina to sufficient levels to induce demyelination following intranasal inoculation. Intracranial inoculation should continue to be considered a preferred method for studies of MHV-induced optic neuritis and central nervous system (CNS) demyelinating disease.
has issue date	2018-09-04(xsd:dateTime)
bibo:doi	10.3389/fcimb.2018.00311
bibo:pmid	30234031
has license	cc-by
sha1sum (hex)	eb65ef8d3ff24ddb51c4f091a46394f513a9d4d9
schema:url	https://doi.org/10.3389/fcimb.2018.00311
resource representing a document's title	Intracranial Inoculation Is More Potent Than Intranasal Inoculation for Inducing Optic Neuritis in the Mouse Hepatitis Virus-Induced Model of Multiple Sclerosis
has PubMed Central identifier	PMC6132074
has PubMed identifier	30234031
schema:publication	Front Cell Infect Microbiol
resource representing a document's body	covid:eb65ef8d3ff24ddb51c4f091a46394f513a9d4d9#body_text
is schema:about of	named entity 'inoculation' named entity 'week' named entity 'demyelinating disease' named entity 'effects' named entity 'One' named entity 'confirm' named entity 'chronic' named entity 'induce' named entity 'inoculation' named entity 'demyelinating disease' named entity 'inoculation' named entity 'Intranasal' named entity 'DOSES' named entity 'INDUCED' named entity 'INTRANASAL INOCULATION' named entity 'SUGGEST' named entity 'PREFERRED' named entity 'LOSS OF' named entity 'METHOD' named entity 'COMPARED' named entity 'OPTIC NERVE' named entity 'AS EXPECTED' named entity 'GIVEN' named entity 'SIGNIFICANT' named entity 'INTRACRANIAL' named entity 'C57BL' named entity 'POST' named entity 'EYE' named entity 'VIRAL' named entity 'EQUIVALENT' named entity 'AXONAL TRANSPORT' named entity 'ACCUMULATION' named entity 'AXONAL' named entity 'MULTIPLE SCLEROSIS' named entity 'DID' named entity 'MOUSE HEPATITIS VIRUS' named entity 'INDUCED' named entity 'CONFIRM' named entity 'FEATURES' named entity 'RAPID' named entity 'PROTEINS' named entity 'HIGHER' named entity 'CONSIDERED' named entity 'CONTROL' named entity 'DOES NOT' named entity 'FOUND' named entity 'INOCULATION' named entity 'INTRACRANIAL' named entity 'OPTIC NEURITIS' named entity 'INDUCING' named entity 'INOCULATION' named entity 'INDUCING' named entity 'HUMAN' named entity 'MICE' named entity 'PATHOLOGIC' named entity 'SPREAD' named entity 'SIMILAR' named entity 'SUFFICIENT' named entity 'VIRAL ANTIGEN' named entity 'EXAMINED' named entity 'OLD' named entity 'NON-' named entity 'DEMYELINATION' named entity 'BRAIN' named entity 'FOLLOWING' named entity 'LOW' named entity 'CONCENTRATIONS' named entity 'LEVELS' named entity 'INTRANASAL ADMINISTRATION' named entity 'FAILED' named entity 'SPINAL CORD' named entity 'MYELIN LOSS' named entity 'MOUSE HEPATITIS VIRUS' named entity 'RETINAL GANGLION CELLS' named entity 'OPTIC NEURITIS'

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