About: An siRNA screen for ATG protein depletion reveals the extent of the unconventional functions of the autophagy proteome in virus replication

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About: An siRNA screen for ATG protein depletion reveals the extent of the unconventional functions of the autophagy proteome in virus replication Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An siRNA screen for ATG protein depletion reveals the extent of the unconventional functions of the autophagy proteome in virus replication
Creator	Reggiori, Fulvio Zhou, Xingdong Ahola, Tero Mauthe, Mario Behrends, Christian Stork, Björn Egan, Dave Jones, Alex Jung, Jennifer Langereis, Martijn Mokry, Michal Omta, Wienand De Haan, Cornelis Tooze, Sharon Van Kuppeveld, Frank Paludan, Søren
Source	PMC
abstract	Autophagy is a catabolic process regulated by the orchestrated action of the autophagy-related (ATG) proteins. Recent work indicates that some of the ATG proteins also have autophagy-independent roles. Using an unbiased siRNA screen approach, we explored the extent of these unconventional functions of ATG proteins. We determined the effects of the depletion of each ATG proteome component on the replication of six different viruses. Our screen reveals that up to 36% of the ATG proteins significantly alter the replication of at least one virus in an unconventional fashion. Detailed analysis of two candidates revealed an undocumented role for ATG13 and FIP200 in picornavirus replication that is independent of their function in autophagy as part of the ULK complex. The high numbers of unveiled ATG gene-specific and pathogen-specific functions of the ATG proteins calls for caution in the interpretation of data, which rely solely on the depletion of a single ATG protein to specifically ablate autophagy.
has issue date	2016-08-29(xsd:dateTime)
bibo:doi	10.1083/jcb.201602046
bibo:pmid	27573464
has license	cc-by-nc-sa
sha1sum (hex)	ecf580f9baf30b1e7c860068c834f34d175dbda0
schema:url	https://doi.org/10.1083/jcb.201602046
resource representing a document's title	An siRNA screen for ATG protein depletion reveals the extent of the unconventional functions of the autophagy proteome in virus replication
has PubMed Central identifier	PMC5004442
has PubMed identifier	27573464
schema:publication	J Cell Biol
resource representing a document's body	covid:ecf580f9baf30b1e7c860068c834f34d175dbda0#body_text
is schema:about of	named entity 'FUNCTIONS' named entity 'SCREEN' named entity 'EXTENT OF' named entity 'PROTEIN ' named entity 'VIRUS REPLICATION' named entity 'DEPLETION' named entity 'SIRNA' named entity 'REVEALS' named entity 'PROTEOME' named entity 'ATG PROTEIN' named entity 'AUTOPHAGY' named entity 'UNCONVENTIONAL' named entity 'replication' named entity 'autophagy' named entity 'proteome' named entity 'autophagy' named entity 'siRNA' named entity 'viral replication' named entity 'membrane vesicles' named entity 'cell lines' named entity 'gene knockdown' named entity 'Western blot' named entity 'EMCV' named entity 'siRNAs' named entity 'ATG13' named entity 'infection' named entity 'gene' named entity 'ATG13' named entity 'doxycycline' named entity 'HeLa' named entity 'ULK1' named entity 'ATG7' named entity 'trypsin' named entity 'autophagy' named entity 'transfected' named entity 'viral replication' named entity 'ATG13' named entity 'ATG13' named entity 'viral proteins' named entity 'ATG13' named entity 'virus' named entity 'antiviral' named entity 'scatterplot' named entity 'image acquisition' named entity 'ATG13' named entity 'African green monkey' named entity 'siRNA' named entity 'organelles' named entity 'transfected' named entity 'viruses' named entity 'autophagy' named entity 'cell lines' named entity 'Alexa Fluor' named entity 'LC3' named entity 'ATG13' named entity 'EMCV' named entity 'protein' named entity 'titer' named entity 'SYBR Green' named entity 'streptomycin' named entity 'p62' named entity 'phosphatidylinositol-3-phosphate' named entity 'siRNA' named entity 'wild type' named entity 'catabolic process' named entity 'LC3'

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