About: SARS-CoV-2 detection from nasopharyngeal swab samples without RNA extraction

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About: SARS-CoV-2 detection from nasopharyngeal swab samples without RNA extraction Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS-CoV-2 detection from nasopharyngeal swab samples without RNA extraction
Creator	Gaggero, Aldo Soto-Rifo, Ricardo Valiente-Echeverría, Fernando Barriga, Gonzalo Márquez, Chantal Beltrán-Pavez, Carolina Muñoz, Gabriela
Source	BioRxiv
abstract	The ongoing COVID-19 pandemic has reached more than 200 countries and territories worldwide. Given the large requirement of SARS-CoV-2 diagnosis and considering that RNA extraction kits are in short supply, we investigated whether two commercial RT-qPCR kits were compatible with direct SARS-CoV-2 detection from nasopharyngeal swab samples. We show that one of the tested kits is fully compatible with direct SARS-CoV-2 detection suggesting that omission of an RNA extraction step should be considered in SARS-CoV-2 diagnosis.
has issue date	2020-03-30(xsd:dateTime)
bibo:doi	10.1101/2020.03.28.013508
has license	biorxiv
sha1sum (hex)	ed058bb28b50fb8da84ab9d5c47972b0ce61638d
schema:url	https://doi.org/10.1101/2020.03.28.013508
resource representing a document's title	SARS-CoV-2 detection from nasopharyngeal swab samples without RNA extraction
schema:publication	bioRxiv
resource representing a document's body	covid:ed058bb28b50fb8da84ab9d5c47972b0ce61638d#body_text
is schema:about of	named entity 'COUNTRIES' named entity 'CONSIDERING' named entity '200' named entity 'kits' named entity 'pandemic' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'COVID' named entity 'Universidad de Chile' named entity 'RNA extraction' named entity 'COVID' named entity 'RNA' named entity 'Nasopharyngeal swabs' named entity 'RNA extraction' named entity 'low-income countries' named entity 'COVID-19 outbreak' named entity 'Thermo Fisher Scientific' named entity 'virus' named entity 'RT-qPCR' named entity 'Thermo Fisher Scientific' named entity 'human coronavirus' named entity 'qPCR' named entity 'ThermoFisher' named entity '2019-nCoV' named entity 'RNA' named entity 'RNA' named entity 'nasopharyngeal swabs' named entity 'RNA' named entity 'gene' named entity 'COVID' named entity 'COVID' named entity 'low-income countries' named entity 'Santiago' named entity 'SARS-CoV-2' named entity 'pneumonia' named entity 'SARS-CoV-2' named entity 'RNA' named entity 'CDC' named entity 'negative control' named entity 'coronaviruses' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'RT-qPCR' named entity 'RNA extraction' named entity 'RNA extraction' named entity 'SARS-CoV-2' named entity 'COVID' named entity 'viral RNA' named entity 'RNA extraction' named entity 'countries and territories' named entity 'RT-qPCR' named entity 'deidentified' named entity 'severe acute respiratory syndrome coronavirus' named entity 'qPCR' named entity 'Integrated DNA Technologies' named entity 'SARS-CoV-2' named entity 'virus' named entity 'SARS-CoV-2' named entity 'positive control' named entity 'HEK293T' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'nasopharyngeal swab' named entity 'COVID' named entity '1, 2' named entity 'Chile' named entity 'SARS-CoV-2' named entity 'COVID' named entity 'CDC' named entity 'CDC' named entity '2019-nCoV' named entity 'MERS-CoV' named entity 'gene' named entity 'SARS-CoV-2 virus' named entity 'COVID' named entity 'José Joaquín Aguirre' named entity 'Integrated DNA Technologies'

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