About: Study on inactivation of porcine epidemic diarrhoea virus, porcine sapelovirus 1 and adenovirus in the production and storage of laboratory spray‐dried porcine plasma

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About: Study on inactivation of porcine epidemic diarrhoea virus, porcine sapelovirus 1 and adenovirus in the production and storage of laboratory spray‐dried porcine plasma Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Study on inactivation of porcine epidemic diarrhoea virus, porcine sapelovirus 1 and adenovirus in the production and storage of laboratory spray‐dried porcine plasma
Creator	Fox, M Hakze-Van Der Honing, R Heres, L Hulst, M Pelser, M Van Der Poel, W
Source	PMC
abstract	AIM: Evaluation of the thermal and physical conditions for inactivation of adenovirus (AdV), porcine sapelovirus 1 (PSV1) and the economically important viruses porcine epidemic diarrhoea virus (PEDV) and porcine circovirus 2 (PCV2) in the production of spray‐dried porcine plasma (SDPP). METHODS AND RESULTS: Citrate‐treated porcine plasma of pH 7·5, 9·8 and 10·2 (8·5% dry‐matter) was spiked with PEDV, PSV1, PCV2 and AdV and incubated at 3°C for maximum 24 h, and at 44 or 48°C for maximum 10 min (Experiment 1). Spiked citrate‐treated concentrated plasma of pH 7·5 and 9·8 (24% dry‐matter) was spray dried in a laboratory scale apparatus (Experiment 2). Aliquots of SDPP were stored over a period of 0–10 weeks at 11 and 20°C (Experiment 3). Reverse transcription(RT)‐quantitative PCR detected no notable reduction in viral genomes in treated plasma and SDPP samples. No infectious PSV1 was re‐isolated from plasma and SDPP samples in cell culture. At pH 10·2 and 3°C, infectivity of PEDV in plasma was reduced with a reduction factor of 4·2 log 10 (LRF) at 10 h contact time, whereas heating to 44°C for at least 1 min at alkali pH was needed to achieve a LRF of 4·2 for AdV. Spray drying at an outlet temperature of 80°C reduced AdV infectivity effectively (LRF = 5·2) and PEDV infectivity for 95% (LRF = 1·4). After storage at 20°C for 2 weeks no infectious PEDV was re‐isolated from SDPP anymore (LRF ≥4·0). Due to growth of antibiotic‐resistant bacteria from plasma in cell cultures used for PCV2 isolation, no data regarding inactivation of PCV2 were obtained. CONCLUSIONS: Five percent of PEDV stayed infectious after our spray drying conditions. Spray drying in combination with storage for ≥2 weeks at 20°C eliminated infectivity of PEDV effectively. SIGNIFICANCE AND IMPACT OF THE STUDY: The conditions for inactivation of virus in plasma and SDPP determined are important for producers to inactivate PEDV during production of SDPP.
has issue date	2019-04-01(xsd:dateTime)
bibo:doi	10.1111/jam.14235
bibo:pmid	30803120
has license	cc-by-nc-nd
sha1sum (hex)	ed6b1dc674893e435945a5efbd1eef13a7c56bc1
schema:url	https://doi.org/10.1111/jam.14235
resource representing a document's title	Study on inactivation of porcine epidemic diarrhoea virus, porcine sapelovirus 1 and adenovirus in the production and storage of laboratory spray‐dried porcine plasma
has PubMed Central identifier	PMC6849764
has PubMed identifier	30803120
schema:publication	J Appl Microbiol
resource representing a document's body	covid:ed6b1dc674893e435945a5efbd1eef13a7c56bc1#body_text
is schema:about of	named entity 'PORCINE EPIDEMIC DIARRHOEA VIRUS' named entity 'FEED' named entity 'ADENOVIRUS' named entity 'THERMAL' named entity 'plasma' named entity 'PORCINE EPIDEMIC DIARRHOEA VIRUS' named entity 'DRIED' named entity 'STUDY' named entity 'KEYWORDS' named entity 'SPRAY' covid:arg/ed6b1dc674893e435945a5efbd1eef13a7c56bc1 named entity 'PORCINE SAPELOVIRUS 1' named entity 'LABORATORY' named entity 'PORCINE' named entity 'PRODUCTION' named entity 'STORAGE' named entity 'PORCINE' named entity 'INACTIVATION' named entity 'ADENOVIRUS' named entity 'PHYSICAL' named entity 'SPRAY' named entity 'INACTIVATION' named entity 'PLASMA' named entity 'PLASMA' named entity 'VIRUS' named entity 'DRIED' named entity 'SAFETY' named entity 'porcine' named entity 'adenovirus' named entity 'porcine epidemic diarrhoea' named entity 'plasma' named entity 'virus' named entity 'porcine epidemic diarrhoea' named entity 'virus' named entity 'PCV2' named entity 'EMEM' named entity 'viral genomes' named entity 'culture medium' named entity 'AdV' named entity 'spike proteins' named entity 'PCV2' named entity 'PCV2' named entity 'DNA virus' named entity 'genomes' named entity 'RNA' named entity 'virus' named entity 'PEDV' named entity 'PEDV' named entity 'Vero cells' named entity 'virus' named entity 'qPCR' named entity 'seroconversion' named entity 'PEDV' named entity 'virus' named entity 'qPCR' named entity 'virus infection' named entity 'PEDV' named entity 'microscope' named entity 'Netherlands' named entity 'AdV' named entity 'qPCR' named entity 'PEDV' named entity 'PCV2' named entity 'w/v' named entity 'PEDV' named entity 'spray drying' named entity 'filtration' named entity 'virus' named entity 'PEDV' named entity 'multiplicity of infection' named entity 'Vero' named entity 'infectivity' named entity 'spray drying' named entity 'AdV' named entity 'alkali' named entity 'RNases'

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