About: Isothermal Amplification Using a Chemical Heating Device for Point-of-Care Detection of HIV-1

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Isothermal Amplification Using a Chemical Heating Device for Point-of-Care Detection of HIV-1 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Isothermal Amplification Using a Chemical Heating Device for Point-of-Care Detection of HIV-1
Creator	Singleton, Jered Weigl, Bernhard Beddoe, Andy Nejad, Irene Curtis, Kelly Labarre, Paul Owen, S Rudolph, Donna
Source	PMC
abstract	BACKGROUND: To date, the use of traditional nucleic acid amplification tests (NAAT) for detection of HIV-1 DNA or RNA has been restricted to laboratory settings due to time, equipment, and technical expertise requirements. The availability of a rapid NAAT with applicability for resource-limited or point-of-care (POC) settings would fill a great need in HIV diagnostics, allowing for timely diagnosis or confirmation of infection status, as well as facilitating the diagnosis of acute infection, screening and evaluation of infants born to HIV-infected mothers. Isothermal amplification methods, such as reverse-transcription, loop-mediated isothermal amplification (RT-LAMP), exhibit characteristics that are ideal for POC settings, since they are typically quicker, easier to perform, and allow for integration into low-tech, portable heating devices. METHODOLOGY/SIGNIFICANT FINDINGS: In this study, we evaluated the HIV-1 RT-LAMP assay using portable, non-instrumented nucleic acid amplification (NINA) heating devices that generate heat from the exothermic reaction of calcium oxide and water. The NINA heating devices exhibited stable temperatures throughout the amplification reaction and consistent amplification results between three separate devices and a thermalcycler. The performance of the NINA heaters was validated using whole blood specimens from HIV-1 infected patients. CONCLUSION: The RT-LAMP isothermal amplification method used in conjunction with a chemical heating device provides a portable, rapid and robust NAAT platform that has the potential to facilitate HIV-1 testing in resource-limited settings and POC.
has issue date	2012-02-23(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0031432
bibo:pmid	22384022
has license	cc0
sha1sum (hex)	ef7110a9022bac2e50c995b0f6b826ff071e48f8
schema:url	https://doi.org/10.1371/journal.pone.0031432
resource representing a document's title	Isothermal Amplification Using a Chemical Heating Device for Point-of-Care Detection of HIV-1
has PubMed Central identifier	PMC3285652
has PubMed identifier	22384022
schema:publication	PLoS One
resource representing a document's body	covid:ef7110a9022bac2e50c995b0f6b826ff071e48f8#body_text
is schema:about of	named entity 'SETTINGS' named entity 'HEATING' named entity 'INFECTION STATUS' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'DNA' named entity 'NUCLEIC ACID AMPLIFICATION' named entity 'PERFORM' named entity 'RAPID' named entity 'MOTHERS' named entity 'METHODS' named entity 'EXHIBIT' named entity 'FACILITATING' named entity 'LOW' named entity 'POINT-OF-CARE' named entity 'HIV-1' named entity 'RESTRICTED' named entity 'ALLOWING' named entity 'BACKGROUND' named entity 'REQUIREMENTS' named entity 'CONFIRMATION OF' named entity 'RNA' named entity 'DIAGNOSTICS' named entity 'EVALUATION' named entity 'EQUIPMENT' named entity 'HIV-1' named entity 'NAAT' named entity 'LABORATORY' named entity 'ALLOW' named entity 'INFECTED' named entity 'IDEAL' named entity 'HEATING DEVICE' named entity 'CHEMICAL' named entity 'AMPLIFICATION' named entity 'USING' named entity 'DIAGNOSIS' named entity 'INTEGRATION' named entity 'CHARACTERISTICS' named entity 'POINT-OF-CARE' named entity 'POC' named entity 'RESOURCE' named entity 'BORN' named entity 'USE OF' named entity 'TIME' named entity 'GREAT' named entity 'INFANTS' named entity 'SCREENING' named entity 'DATE' named entity 'NEED' named entity 'DEVICES' named entity 'DETECTION' named entity 'AMPLIFICATION' named entity 'LIMITED' named entity 'TIMELY' named entity 'HIV' named entity 'TECHNICAL' named entity 'EXPERTISE' named entity 'AVAILABILITY OF' named entity 'TECH' named entity 'ACUTE INFECTION' named entity 'LAMP' named entity 'DETECTION' named entity 'PORTABLE' named entity 'TRADITIONAL' named entity 'DUE TO' named entity 'TESTS' covid:arg/ef7110a9022bac2e50c995b0f6b826ff071e48f8 named entity 'POC' named entity 'ideal' named entity 'amplification' named entity 'Isothermal' named entity 'POC' named entity 'infants' named entity 'great' named entity 'settings'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software