About: Glucose-Regulated Protein 78 Interacts with Zika Virus Envelope Protein and Contributes to a Productive Infection

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About: Glucose-Regulated Protein 78 Interacts with Zika Virus Envelope Protein and Contributes to a Productive Infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Glucose-Regulated Protein 78 Interacts with Zika Virus Envelope Protein and Contributes to a Productive Infection
Creator	Merits, Andres Royle, Jamie Kohl, Alain Varjak, Margus Donald, Claire Anaya, Juan-Manuel Ramírez-Santana, Carolina Akpunarlieva, Snezhana Burchmore, Richard Gestuveo, Rommel
source	Medline; PMC
abstract	Zika virus (ZIKV; Flaviviridae) is a mosquito-borne flavivirus shown to cause fetal abnormalities collectively known as congenital Zika syndrome and Guillain-Barré syndrome in recent outbreaks. Currently, there is no specific treatment or vaccine available, and more effort is needed to identify cellular factors in the viral life cycle. Here, we investigated interactors of ZIKV envelope (E) protein by combining protein pull-down with mass spectrometry. We found that E interacts with the endoplasmic reticulum (ER) resident chaperone, glucose regulated protein 78 (GRP78). Although other flaviviruses are known to co-opt ER resident proteins, including GRP78, to enhance viral infectivity, the role ER proteins play during the ZIKV life cycle is yet to be elucidated. We showed that GRP78 levels increased during ZIKV infection and localised to sites coincident with ZIKV E staining. Depletion of GRP78 using specific siRNAs significantly reduced reporter-virus luciferase readings, viral protein synthesis, and viral titres. Additionally, GRP78 depletion reduced the ability of ZIKV to disrupt host cell translation and altered the localisation of viral replication factories, though there was no effect on viral RNA synthesis. In summary, we showed GRP78 is a vital host-factor during ZIKV infection, which may be involved in the coordination of viral replication factories.
has issue date	2020-05-09(xsd:dateTime)
bibo:doi	10.3390/v12050524
bibo:pmid	32397571
has license	cc-by
sha1sum (hex)	efbdbec86374f0d9a8c0f5a1b7890b1c9ff8933c
schema:url	https://doi.org/10.3390/v12050524
resource representing a document's title	Glucose-Regulated Protein 78 Interacts with Zika Virus Envelope Protein and Contributes to a Productive Infection
has PubMed Central identifier	PMC7290722
has PubMed identifier	32397571
schema:publication	Viruses
resource representing a document's body	covid:efbdbec86374f0d9a8c0f5a1b7890b1c9ff8933c#body_text
is schema:about of	named entity 'mass spectrometry' named entity 'Depletion' named entity 'collectively' named entity 'factories' named entity 'MAY BE' named entity 'ENVELOPE' named entity 'SYNDROME' named entity 'BORNE' named entity 'INVESTIGATED' named entity 'DELINEATE' named entity 'REDUCED' named entity 'RNA SYNTHESIS' named entity 'EFFORT' named entity 'ZIKA VIRUS' named entity 'SITES' named entity 'KNOWN' named entity 'NEEDED' named entity 'OPT' named entity 'ROLE' named entity 'co-opt' named entity 'viral replication' named entity 'infectivity' named entity 'infection' named entity 'investigated' named entity 'resident' named entity 'infection' named entity 'regulated' named entity 'specific' named entity 'flavivirus' named entity 'fetal' named entity 'protein synthesis' named entity 'ZIKV' named entity 'ZIKV' named entity 'flavivirus' named entity 'GRP78' named entity 'flaviviruses' named entity 'Guillain-Barré syndrome' named entity 'life cycle' named entity 'ZIKV' named entity 'mass spectrometry' named entity 'virus' named entity 'viral life cycle' named entity 'Envelope Protein' named entity 'Infection' named entity 'Glucose-Regulated Protein' named entity 'GRP78' named entity 'viral particles' named entity 'GRP78' named entity 'false discovery rate' named entity 'dsRNA' named entity 'A549' named entity 'Abcam' named entity 'supernatant' named entity 'virus' named entity 'statistical significance' named entity 'A549' named entity 'protein' named entity 'DENV' named entity 'GRP78' named entity 'Dulbecco' named entity 'mosquito-borne virus' named entity 'West Nile virus' named entity 'cytomegalovirus' named entity 'primers' named entity 'virus' named entity 'type 1 interferon' named entity 'ribosomes' named entity 'antibodies' named entity 'lysed' named entity 'MOI' named entity 'receptor' named entity 'MOI'

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