About: Real-time Fluorescent PCR Techniques to Study Microbial

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Real-time Fluorescent PCR Techniques to Study Microbial–Host Interactions
Creator	Nitsche, Andreas Arden, Katherine Mackay, Ian
Source	Elsevier; PMC
abstract	Publisher Summary This chapter describes how real-time polymerase chain reaction (PCR) performs and how it may be used to detect microbial pathogens and the relationship they form with their host. Research and diagnostic microbiology laboratories contain a mix of traditional and leading-edge, in-house and commercial assays for the detection of microbes and the effects they impart upon target tissues, organs, and systems. The PCR has undergone significant change over the last decade, to the extent that only a small proportion of scientists have been able or willing to keep abreast of the latest offerings. The chapter reviews these changes. It discusses the second-generation of PCR technology—kinetic or real-time PCR, a tool gaining widespread acceptance in many scientific disciplines but especially in the microbiology laboratory.
has issue date	2004-12-31(xsd:dateTime)
bibo:doi	10.1016/s0580-9517(04)34010-9
has license	els-covid
sha1sum (hex)	f0c20d0d5209adaa88bd6f874f6c75f3e09eb24b
schema:url	https://doi.org/10.1016/s0580-9517%2804%2934010-9
resource representing a document's title	Real-time Fluorescent PCR Techniques to Study Microbial–Host Interactions
has PubMed Central identifier	PMC7148886
schema:publication	Methods in Microbiology
resource representing a document's body	covid:f0c20d0d5209adaa88bd6f874f6c75f3e09eb24b#body_text
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