About: A handheld point-of-care system for rapid detection of SARS-CoV-2 in under 20 minutes

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About: A handheld point-of-care system for rapid detection of SARS-CoV-2 in under 20 minutes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A handheld point-of-care system for rapid detection of SARS-CoV-2 in under 20 minutes
Creator	Barclay, Wendy Holmes, Alison Bolt, Frances Cavuto, Matthew Davies, Frances Georgiou, Pantelis Malpartida-Cardenas, Kenny Miglietta, Luca Moniri, Ahmad Moser, Nicolas Penn, Rebecca Pennisi, Ivana Randell, Paul Rodriguez-Manzano, Jesus Satta, Giovanni
Source	MedRxiv
abstract	The COVID-19 pandemic is a global health emergency characterized by the high rate of transmission and ongoing increase of cases globally. Rapid point-of-care (PoC) diagnostics to detect the causative virus, SARS-CoV-2, are urgently needed to identify and isolate patients, contain its spread and guide clinical management. In this work, we report the development of a rapid PoC diagnostic test (< 20 min) based on reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) and semiconductor technology for the detection of SARS-CoV-2 from extracted RNA samples. The developed LAMP assay was tested on a real-time benchtop instrument (RT-qLAMP) showing a lower limit of detection of 10 RNA copies per reaction. It was validated against 183 clinical samples including 127 positive samples (screened by the CDC RT-qPCR assay). Results showed 90.55% sensitivity and 100% specificity when compared to RT-qPCR and average positive detection times of 15.45 {+/-} 4.43 min. For validating the incorporation of the RT-LAMP assay onto our PoC platform (RT-eLAMP), a subset of samples was tested (n=40), showing average detection times of 12.89 {+/-} 2.59 min for positive samples (n=34), demonstrating a comparable performance to a benchtop commercial instrument. Paired with a smartphone for results visualization and geo-localization, this portable diagnostic platform with secure cloud connectivity will enable real-time case identification and epidemiological surveillance.
has issue date	2020-06-30(xsd:dateTime)
bibo:doi	10.1101/2020.06.29.20142349
has license	medrxiv
sha1sum (hex)	f3708f412b2c048be196e2da8ae03b26f476c5f5
schema:url	https://doi.org/10.1101/2020.06.29.20142349
resource representing a document's title	A handheld point-of-care system for rapid detection of SARS-CoV-2 in under 20 minutes
resource representing a document's body	covid:f3708f412b2c048be196e2da8ae03b26f476c5f5#body_text
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