About: ExoU-induced redox imbalance and oxidative stress in airway epithelial cells during Pseudomonas aeruginosa pneumosepsis

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About: ExoU-induced redox imbalance and oxidative stress in airway epithelial cells during Pseudomonas aeruginosa pneumosepsis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	ExoU-induced redox imbalance and oxidative stress in airway epithelial cells during Pseudomonas aeruginosa pneumosepsis
Creator	Barja-Fidalgo, Christina Castro-Faria-Neto, Caire Cunha, Da De Moraes, Alfredo Ferreira, Francisca Gonzaga, Luiz Hugo, · João, · Mattos Saliba, Alessandra Miriam, · Patricia, · Plotkowski, Maria-Cristina Reis, Alves
source	PMC
abstract	ExoU is a potent proinflammatory toxin produced by Pseudomonas aeruginosa, a major agent of severe lung infection and sepsis. Because inflammation is usually associated with oxidative stress, we investigated the effect of ExoU on free radical production and antioxidant defense mechanisms during the course of P. aeruginosa infection. In an experimental model of acute pneumonia, ExoU accounted for increased lipid peroxidation in mice lungs as soon as 3 h after intratracheal instillation of PA103 P. aeruginosa strain. The contribution of airway cells to the generation of a redox imbalance was assessed by in vitro tests carried out with A549 airway epithelial cells. Cultures infected with the ExoU-producing PA103 P. aeruginosa strain produced significantly increased concentrations of lipid hydroperoxides, 8-isoprostane, reactive oxygen intermediates, peroxynitrite and nitric oxide (NO), when compared to cells infected with exoU-deficient mutants. Overproduction of NO by PA103-infected cells likely resulted from overexpression of both inducible and endothelial NO synthase isoforms. PA103 infection was also associated with a significantly increased activity of superoxide dismutase (SOD) and decreased levels of reduced glutathione (GSH), a major antioxidant compound. Our findings unveil another potential mechanism of tissue damage during infection by ExoU-producing P. aeruginosa strains.
has issue date	2015-04-23(xsd:dateTime)
bibo:doi	10.1007/s00430-015-0418-x
bibo:pmid	25904542
has license	no-cc
sha1sum (hex)	f3e93a207517eb950db56c495bc9921904d5d61f
schema:url	https://doi.org/10.1007/s00430-015-0418-x
resource representing a document's title	ExoU-induced redox imbalance and oxidative stress in airway epithelial cells during Pseudomonas aeruginosa pneumosepsis
has PubMed Central identifier	PMC7100071
has PubMed identifier	25904542
schema:publication	Med Microbiol Immunol
resource representing a document's body	covid:f3e93a207517eb950db56c495bc9921904d5d61f#body_text
is schema:about of	named entity 'epithelial cells' named entity 'P. aeruginosa' named entity 'oxidative stress' named entity 'protein' named entity 'cell surfaces' named entity 'nNOS' named entity '37 °C' named entity 'infection' named entity 'breakdown products' named entity 'cellular signaling' named entity 'mice' named entity 'ROS' named entity 'RNS' named entity 'milk' named entity 'toxin' named entity 'cell division' named entity 'antioxidant' named entity 'EGTA' named entity 'tissue culture' named entity 'TBARS' named entity 'neutrophil' named entity 'centrifuged' named entity 'culture medium' named entity 'cell cultures' named entity 'iNOS' named entity 'phosphate buffer' named entity 'iNOS' named entity 'GSH' named entity 'superoxide anion' named entity 'ROS' named entity 'State University of Rio de Janeiro' named entity 'antioxidant enzymes' named entity 'loading control' named entity 'cell types' named entity 'oxygen' named entity 'redox' named entity 'P. aeruginosa' named entity 'nitric oxide' named entity 'lipid peroxidation' named entity 'lipid hydroperoxides' named entity 'Lipid peroxidation' named entity 'bacteria' named entity 'lipid hydroperoxides' named entity 'cofactors' named entity 'LPS' named entity 'type III secretion' named entity 'Cayman Chemical' named entity 'bacteria' named entity 'apoptosis' named entity 'lungs' named entity 'severe pneumonia' named entity 'saline' named entity 'alveolar' named entity 'mice' named entity 'upregulate' named entity 'oxidative stress' named entity '0.01' named entity 'nitric oxide' named entity 'primary antibodies' named entity 'permeability' named entity 'wildtype' named entity 'oxygen' named entity 'oxidant' named entity 'P. aeruginosa' named entity 'antioxidant' named entity 'Molecular Probes' named entity 'NF-κB' named entity '4-methylphenol' named entity 'P. aeruginosa'

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