About: The VPgPro protein of Turnip mosaic virus: In vitro inhibition of translation from a ribonuclease activity

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About: The VPgPro protein of Turnip mosaic virus: In vitro inhibition of translation from a ribonuclease activity Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The VPgPro protein of Turnip mosaic virus: In vitro inhibition of translation from a ribonuclease activity
Creator	Cotton, Sophie Ide, Christine Thivierge, Karine Fortin, Marc Dufresne, Philippe
Source	Elsevier; Medline; PMC
abstract	Abstract A role for viral encoded genome-linked (VPg) proteins in translation has often been suggested because of their covalent attachment to the 5′ end of the viral RNA, reminiscent of the cap structure normally present on most eukaryotic mRNAs. We tested the effect of Turnip mosaic virus (TuMV) VPgPro on translation of reporter RNAs in in vitro translation systems. The presence of VPgPro in either wheat germ extract or rabbit reticulocyte lysate systems lead to inhibition of translation. The inhibition did not appear to be mediated by the interaction of VPg with the eIF(iso)4E translation initiation factor since a VPg mutant that does not interact with eIF(iso)4E still inhibited translation. Monitoring the fate of RNAs revealed that they were degraded as a result of addition of TuMV VPgPro or of Norwalk virus (NV) VPg protein. The RNA degradation was not the result of translation being arrested and was heat labile and partially EDTA sensitive. The capacity of TuMV VPgPro and of (NV) VPg to degrade RNA suggests that these proteins have a ribonucleolytic activity which may contribute to the host RNA translation shutoff associated with many virus infections.
has issue date	2006-07-20(xsd:dateTime)
bibo:doi	10.1016/j.virol.2006.03.019
bibo:pmid	16647732
has license	els-covid
sha1sum (hex)	f3f5ed3d7a80bf794726a1cb9234df3f361beb2b
schema:url	https://doi.org/10.1016/j.virol.2006.03.019
resource representing a document's title	The VPgPro protein of Turnip mosaic virus: In vitro inhibition of translation from a ribonuclease activity
has PubMed Central identifier	PMC7172391
has PubMed identifier	16647732
schema:publication	Virology
resource representing a document's body	covid:f3f5ed3d7a80bf794726a1cb9234df3f361beb2b#body_text
is schema:about of	named entity 'Norwalk virus' named entity 'virus' named entity 'degradation' named entity 'translation' named entity 'In vitro' named entity '80%' named entity 'EUKARYOTIC' named entity 'VIRUS' named entity 'The presence' named entity 'VPg' named entity 'inhibited' named entity 'extract' named entity 'fate' named entity 'host' named entity 'RNAs' named entity 'RNA' named entity 'VPg' named entity 'GST' named entity 'E. coli' named entity 'GST' named entity 'infection' named entity '4.3' named entity 'virus' named entity 'result' named entity 'inhibition' named entity 'reminiscent' named entity 'inhibition' named entity 'Turnip mosaic virus' named entity 'VPg' named entity '5′ end' named entity 'viral RNA' named entity 'lead' named entity 'GST' named entity 'affinity purification' named entity 'GST' named entity 'mRNA' named entity 'sonication' named entity 'RNA' named entity 'Savithri' named entity 'cDNA' named entity 'luciferase' named entity 'GST' named entity 'virus infection' named entity 'ribonuclease' named entity 'mRNAs' named entity 'VPg' named entity 'fusion protein' named entity 'Bradford assay' named entity 'EDTA' named entity 'citric acid' named entity 'Hulst' named entity 'deoxyribonuclease' named entity 'cycloheximide' named entity 'EDTA' named entity 'infection' named entity 'RNA' named entity 'VPg' named entity 'secondary antibody' named entity 'VPg' named entity 'protein' named entity 'binding protein' named entity 'RNA' named entity 'RNA' named entity 'Influenza virus' named entity 'recombinant' named entity 'virus' named entity 'potyvirus' named entity 'infection' named entity 'positive-sense RNA' named entity 'nuclease' named entity 'RNA' named entity 'GST' named entity 'ribonucleases' named entity 'VPg' named entity 'VPg' named entity 'RNA' named entity 'nucleoprotein complex'

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