About: Analysis using canine peripheral blood for establishing in vitro conditions for monocyte differentiation into macrophages for Leishmania chagasi infection and T-cell subset purification

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About: Analysis using canine peripheral blood for establishing in vitro conditions for monocyte differentiation into macrophages for Leishmania chagasi infection and T-cell subset purification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Analysis using canine peripheral blood for establishing in vitro conditions for monocyte differentiation into macrophages for Leishmania chagasi infection and T-cell subset purification
Creator	Corrêa-Oliveira, Rodrigo Aguiar-Soares, Oliveira Assis Martins-Filho, Olindo Dian, Rodrigo Giunchetti, Rodolfo Mendes Roatt, Bruno Reis, Alexandre Araújo, Silva Da Silveira-Lemos, Denise Sobreira, Márcio Fernandes Viana, Kelvinson Lima Moura, Sandra Resende, Aparecida Santos Zanini, Marcos
source	Elsevier; Medline; PMC
abstract	Abstract Canine visceral leishmaniasis (CVL) is a parasitic disease endemic in many countries, and dogs present as the major natural reservoir of the parasite, Leishmania chagasi (syn. L. infantum). Biomarkers in the canine immune system is an important technique in the course of developing vaccines and treatment strategies against CVL. New methodologies for studying the immune response of dogs during Leishmania infection and after receiving vaccines and treatments against CVL would be useful. In this context, we used peripheral blood mononuclear cells (PBMCs) from healthy dogs to evaluate procedures related to (i) establishment of in vitro conditions of monocytes differentiated into macrophages infected with L. chagasi and (ii) purification procedures of T-cell subsets (CD4+ and CD8+) using microbeads. Our data demonstrated that after 5 days of differentiation, macrophages were able to induce significant phagocytic and microbicidal activity after L. chagasi infection and also showed increased frequency of parasitism and a higher parasite load. Although N-acetyl-β-d-glucosaminidase (NAG) levels presented similar levels of macrophage culture and L. chagasi infection, a progressive decrease in myeloperoxidase (MPO) levels was a hallmark over 5 days of culture. High purity levels (>90%) of CD4 and CD8 T cells were obtained on a magnetic separation column. We concluded that monocytes differentiated into macrophages at 5 days and displayed an intermediate frequency of parasitism and parasite load 72h after L. chagasi infection. Furthermore, the purification system using canine T-lymphocyte subsets obtained after 5 days of monocyte differentiation proved efficient for CD4 or CD8 T-cell purification (≥90%). The in vitro analysis using L. chagasi-infected macrophages and purified T cells presented a prospective methodology that could be incorporated in CVL vaccine and treatment studies that aim to analyze the microbicidal potential induced by specific CD4+ and/or CD8+ T cells.
has issue date	2013-11-15(xsd:dateTime)
bibo:doi	10.1016/j.vetpar.2013.08.014
bibo:pmid	24018185
has license	els-covid
sha1sum (hex)	f61db2492bf5bb245616f61730a6866d3c471e32
schema:url	https://doi.org/10.1016/j.vetpar.2013.08.014
resource representing a document's title	Analysis using canine peripheral blood for establishing in vitro conditions for monocyte differentiation into macrophages for Leishmania chagasi infection and T-cell subset purification
has PubMed Central identifier	PMC7115789
has PubMed identifier	24018185
schema:publication	Veterinary Parasitology
resource representing a document's body	covid:f61db2492bf5bb245616f61730a6866d3c471e32#body_text
is schema:about of	named entity 'immune system' named entity 'parasite' named entity 'infected' named entity 'macrophages' named entity 'studying' named entity 'visceral leishmaniasis' named entity 'ABLE TO' named entity 'LEISHMANIA CHAGASI' named entity 'SUBSET' named entity 'IN VITRO' named entity 'HIGHER' named entity 'T CELLS' named entity 'LEISHMANIA INFECTION' named entity 'RESERVOIR' named entity 'TREATMENTS' named entity 'TECHNIQUE' named entity 'ENDEMIC' named entity 'PRESENT' named entity 'MONOCYTES' named entity 'DOGS' named entity 'MAJOR' named entity 'HEALTHY' named entity '28I' named entity 'LEISHMANIA CHAGASI' named entity 'RELATED' named entity 'INFECTED' named entity 'OUR' named entity 'INFECTION' named entity 'EVALUATE' named entity 'DIFFERENTIATED' named entity 'IS A' named entity 'S T' named entity 'USED' named entity 'COUNTRIES' named entity 'INDUCE' named entity 'STRATEGIES' named entity 'PURIFICATION' named entity 'TREATMENT' named entity 'IN VITRO' named entity 'INCREASED FREQUENCY' named entity 'CANINE' named entity 'IMPORTANT' named entity 'T-CELL SUBSETS' named entity 'COURSE' named entity 'CD8' named entity 'NATURAL' named entity 'PROCEDURES' named entity 'MACROPHAGES' named entity 'VACCINES' named entity 'ESTABLISHING' named entity 'ANALYSIS' named entity 'CONDITIONS' named entity 'USING' named entity 'DATA' named entity 'SIGNIFICANT' named entity 'CANINE VISCERAL LEISHMANIASIS' named entity 'MACROPHAGE' named entity 'CONTEXT' named entity 'MACROPHAGES' named entity 'CANINE' named entity 'USING' named entity 'INFECTION' named entity 'PERIPHERAL BLOOD' named entity 'USEFUL' named entity 'DEVELOPING' named entity 'PARASITIC DISEASE' named entity 'L. INFANTUM' named entity 'BIOMARKERS'

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