About: Down-regulation of granulocyte-macrophage colony-stimulating factor by 3C-like proteinase in transfected A549 human lung carcinoma cells

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About: Down-regulation of granulocyte-macrophage colony-stimulating factor by 3C-like proteinase in transfected A549 human lung carcinoma cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Down-regulation of granulocyte-macrophage colony-stimulating factor by 3C-like proteinase in transfected A549 human lung carcinoma cells
Creator	Chen, Ming Chen, Shui-Tein Cheng, Sun-Long Liao, Hsien-Hua Lin, Johnson Tsai, Hsien-Yu Wang, Yao-Chen Chih-, Miles Tsay, Gregory
Source	Medline; PMC
abstract	BACKGROUND: Severe Acute Respiratory Syndrome (SARS) is a severe respiratory illness caused by a novel virus, the SARS coronavirus (SARS-CoV). 3C-like protease (3CL(pro)) of SARS-CoV plays a role in processing viral polypeptide precursors and is responsible of viral maturation. However, the function of 3CL(pro )in host cells remains unknown. This study investigated how the 3CL(pro )affected the secretion of cytokines in the gene-transfected cells. RESULTS: From immunofluorescence microscopy, the localization of c-myc tagged 3CL(pro )was detected both in the cytoplasm and nucleus of transfected A549 cells. Expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) was significantly decreased in 3CL(pro)-transfected cells by both RT-PCR and ELISA, but without changes in other cytokines, i.e., IL-1β, IL-6, IL-8, IL12p40, TNF-α, and TGF-β. Furthermore, the protein levels of NF-kB decreased in 3CL(pro)-transfected A549 cells when compared to EGFP transfected cells. CONCLUSIONS: Our results suggest that the 3CL(pro )may suppress expression of GM-CSF in transfected A549 cells through down-regulation of NF-kB production.
has issue date	2011-02-17(xsd:dateTime)
bibo:doi	10.1186/1471-2172-12-16
bibo:pmid	21324206
has license	cc-by
sha1sum (hex)	f64a9ce29e9ee3f25d1888ba6748dfdcd1fa3131
schema:url	https://doi.org/10.1186/1471-2172-12-16
resource representing a document's title	Down-regulation of granulocyte-macrophage colony-stimulating factor by 3C-like proteinase in transfected A549 human lung carcinoma cells
has PubMed Central identifier	PMC3048559
has PubMed identifier	21324206
schema:publication	BMC Immunol
resource representing a document's body	covid:f64a9ce29e9ee3f25d1888ba6748dfdcd1fa3131#body_text
is schema:about of	named entity 'GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR' named entity 'This' named entity 'responsible' named entity 'severe' named entity 'virus' named entity 'granulocyte-macrophage colony-stimulating factor' named entity 'cells' named entity 'SARS CORONAVIRUS' named entity 'VIRAL MATURATION' named entity 'C-MYC' named entity 'NUCLEUS' named entity 'CYTOPLASM' named entity 'SECRETION' named entity 'TAGGED' named entity 'CAUSED BY' named entity 'STUDY' named entity 'IL12P40' named entity 'BACKGROUND' named entity 'DETECTED' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'SEVERE' named entity 'IL-6' named entity 'RT-PCR' named entity 'IMMUNOFLUORESCENCE MICROSCOPY' named entity 'INVESTIGATED' named entity 'BUT' named entity 'PROTEASE' named entity 'PRECURSORS' covid:arg/f64a9ce29e9ee3f25d1888ba6748dfdcd1fa3131 named entity 'HUMAN LUNG' named entity 'A549' named entity 'HOW' named entity 'CARCINOMA CELLS' named entity 'PROTEINASE IN' named entity 'LIKE' named entity 'LIKE' named entity 'PRO' named entity 'VIRAL' named entity 'ROLE' named entity 'PROCESSING' named entity 'RESULTS' named entity 'DECREASED' named entity 'RESPONSIBLE' named entity 'EGFP' named entity 'NOVEL' named entity 'RESPIRATORY ILLNESS' named entity 'POLYPEPTIDE' named entity 'AFFECTED' named entity 'NF-KB' named entity 'ELISA' named entity 'CHANGES' named entity 'TNF' named entity 'LOCALIZATION' named entity 'A549 CELLS' named entity 'PROTEIN LEVELS' named entity 'EXPRESSION' named entity 'FUNCTION' named entity 'TGF' named entity 'IL-1' named entity 'CELLS' named entity 'CYTOKINES' named entity 'CSF' named entity 'UNKNOWN' named entity 'GENE' named entity 'GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR' named entity 'VIRUS' named entity 'COMPARED' named entity 'IS A' named entity 'PLAYS' named entity 'LUNG CARCINOMA' named entity 'DOWN-REGULATION' named entity 'IL-8' named entity 'HOST CELLS'

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