About: Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-β

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-β
Creator	Hu, Sen Bu, Zhigao Ge, Jinying Wen, Zhiyuan Cao, Wenyan Chen, Weiye Hu, Qianqian Huang, Kehe Qu, Linmao Wang, Xijun Zhao, Huijun Li, Haobo
source	Elsevier; Medline; PMC
abstract	Abstract A CHO cell clone (CHO-PoIFN-β) with stable porcine IFN-β expression under control of CMV promoter was selected under G418 pressure. In a 25cm2 cell culture flask (5ml culture medium), the cumulative protein yield of recombinant PoIFN-β reached 2.3×106 IU/ml. This cells clone maintained stable expression for at least 20 generations even in the absence of G418 selection pressure. The expressed recombinant PoIFN-β could induce the expression of porcine Mx protein in PK15 cells, and activate the chicken Mx promoter-controlled luciferase reporter gene expression, confirming that the recombinant PoIFN-β has the biological activity of natural porcine type-I interferon. In addition, the recombinant PoIFN-β fully protected PK15 cells against 1000 TCID50 of porcine transmissible gastroenteritis virus and pseudo-rabies virus infection, demonstrating its high potential in therapeutic applications. This is the first report of establishing a mammalian cell line with stable expression of porcine IFN-β.
has issue date	2011-06-30(xsd:dateTime)
bibo:doi	10.1016/j.cyto.2010.12.002
bibo:pmid	21459017
has license	els-covid
sha1sum (hex)	f65309ff855b55d401b33fe3fe052ae285f69bf0
schema:url	https://doi.org/10.1016/j.cyto.2010.12.002
resource representing a document's title	Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-β
has PubMed Central identifier	PMC7128424
has PubMed identifier	21459017
schema:publication	Cytokine
resource representing a document's body	covid:f65309ff855b55d401b33fe3fe052ae285f69bf0#body_text
is schema:about of	named entity 'IFN' named entity 'chicken' named entity 'porcine' named entity 'expression' named entity 'expression' named entity 'recombinant' named entity 'porcine' named entity 'BIOLOGICAL ACTIVITY' named entity 'CONFIRMING' named entity 'PK15' named entity 'CUMULATIVE' named entity 'CULTURE MEDIUM' named entity 'STABLE' named entity 'MAINTAINED' named entity 'LUCIFERASE' named entity 'REPORT' named entity 'CMV PROMOTER' named entity 'CHICKEN' named entity 'ESTABLISHING' named entity 'PSEUDO' named entity 'ACTIVATE' named entity 'APPLICATIONS' named entity 'THERAPEUTIC' named entity 'ITS' named entity 'CONTROLLED' named entity 'CHO CELL' named entity 'CLONE' named entity 'TYPE' named entity 'HIGH LEVEL' named entity 'STABLE' named entity 'EXPRESSION' named entity 'ESTABLISHMENT' named entity 'RECOMBINANT' named entity 'YIELD' named entity 'G418' named entity 'EXPRESSION' named entity 'PROTEIN ' named entity 'PROMOTER' named entity 'UNDER CONTROL' named entity 'CHO CELL' named entity 'INDUCE' named entity 'CELLS' named entity 'FLASK' named entity 'INTERFERON' named entity 'HIGH' named entity 'STABLE EXPRESSION' named entity 'CELL CULTURE' named entity 'PORCINE' named entity 'RABIES VIRUS INFECTION' named entity 'SELECTED' named entity 'FULLY' named entity 'CELL LINE' named entity 'IFN' named entity 'ADDITION' named entity 'TCID' named entity 'POTENTIAL' named entity 'ABSENCE OF' named entity 'EXPRESSED' named entity 'PRESSURE' named entity 'GENE EXPRESSION' named entity 'SELECTION PRESSURE' named entity 'REPORTER GENE' named entity 'NATURAL' named entity 'CELL LINE' named entity 'RECOMBINANT' named entity 'PORCINE TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'GENERATIONS' named entity 'MAMMALIAN CELL' named entity '1000' named entity 'PORCINE'

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