About: A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification

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About: A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification
Creator	Li, Hui Wang, Chen Dillon, Natalie Wong, Freda Pui-Fan Chan, Paul Cheung, Albert Fung, Yin-Wan Lau, Collins, Richard Lin, Sau-Wah Lok, Ting Tam, Siu-Lun Yu, -H Yu, -Hoi
Source	Elsevier; Medline; PMC
abstract	Abstract An enhanced polymerase chain reaction (PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-CoV) was developed in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. Clinical samples were collected from 120 patients diagnosed as suspected or probable SARS cases and analyzed by conventional PCR followed by agarose gel electrophoresis, conventional TaqMan real-time PCR, and our enhanced TaqMan real-time PCR assays. An amplicon of the size expected from SARS-CoV was obtained from 28/120 samples using the enhanced real-time PCR method. Conventional PCR and real-time PCR alone identified fewer SARS-CoV positive cases. Results were confirmed by viral culture in 3/28 cases. The limit of detection of the enhanced real-time PCR method was 102-fold higher than the standard real-time PCR assay and 107-fold higher than conventional PCR methods. The increased sensitivity of the assay may help control the spread of the disease during future SARS outbreaks.
has issue date	2003-12-26(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2003.11.064
bibo:pmid	14652014
has license	els-covid
sha1sum (hex)	f74923b3ce82c984a7ae3e0c2754c9e33c60554f
schema:url	https://doi.org/10.1016/j.bbrc.2003.11.064
resource representing a document's title	A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification
has PubMed Central identifier	PMC7111096
has PubMed identifier	14652014
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:f74923b3ce82c984a7ae3e0c2754c9e33c60554f#body_text
is schema:about of	named entity 'The limit' named entity 'viral culture' named entity 'The' named entity 'assay' named entity 'PRE' named entity 'PROBABLE' named entity 'DIAGNOSED' named entity 'OBTAINED' named entity 'INCREASED SENSITIVITY' named entity 'USING' named entity 'ANALYZED' named entity 'AGAROSE GEL ELECTROPHORESIS' named entity 'POLYMERASE CHAIN REACTION' named entity 'PATIENTS' named entity 'REAL-TIME' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'FOLD' named entity 'TARGET GENE' named entity 'FOLLOWED BY' named entity 'ENHANCED' named entity 'LIMIT OF DETECTION' covid:arg/f74923b3ce82c984a7ae3e0c2754c9e33c60554f named entity 'SUSPECTED' named entity 'TAQMAN' named entity 'DISEASE' named entity 'CORONAVIRUS' named entity 'STANDARD' named entity 'SAMPLES' named entity 'OUR' named entity 'METHODS' named entity 'SIZE' named entity 'FLUORESCENT' named entity 'PCR' named entity 'SARS-COV' named entity 'CONVENTIONAL' named entity 'REAL-TIME PCR' named entity 'ASSAY' named entity 'AMPLICON' named entity 'COLLECTED' named entity 'OUTBREAKS' named entity 'SPREAD' named entity 'REAL-TIME PCR ' named entity 'IDENTIFIED' named entity 'EXPECTED' named entity 'CLINICAL' named entity 'POSITIVE' named entity 'AMPLIFICATION' named entity 'RESULTS' named entity 'CASES' named entity 'FUTURE' named entity 'CONFIRMED BY' named entity 'STEP' named entity 'ASSOCIATED WITH' named entity 'VIRAL CULTURE' named entity 'PRECEDED' named entity '120' named entity 'SARS' named entity 'CONTROL' named entity 'HELP' named entity 'TO DETECT' named entity 'HIGHER' named entity 'agarose gel electrophoresis' named entity 'SARS-CoV' named entity 'fluorescent' named entity 'detection' named entity 'Applied Biosystems' named entity 'clinical data' named entity 'SARS'

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