About: Rapid and Sensitive Detection of Novel Avian-Origin Influenza A (H7N9) Virus by Reverse Transcription Loop-Mediated Isothermal Amplification Combined with a Lateral-Flow Device

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About: Rapid and Sensitive Detection of Novel Avian-Origin Influenza A (H7N9) Virus by Reverse Transcription Loop-Mediated Isothermal Amplification Combined with a Lateral-Flow Device Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Rapid and Sensitive Detection of Novel Avian-Origin Influenza A (H7N9) Virus by Reverse Transcription Loop-Mediated Isothermal Amplification Combined with a Lateral-Flow Device
Creator	Wang, Hua Ge, Yiyue Guo, Xiling Qi, Xian Zhou, Minghao Cui, Lunbiao Qi, Yuhua Shi, Zhiyang Zhao, Kangchen Zhu, Yefei
source	Medline; PMC
abstract	A severe disease in humans caused by a novel avian-origin influenza A (H7N9) virus emerged in China recently, which has caused at least 128 cases and 26 deaths. Rapid detection of the novel H7N9 virus is urgently needed to differentiate the disease from other infections, and to facilitate infection control as well as epidemiologic investigations. In this study, a reverse transcription loop-mediated isothermal amplification combined with a lateral flow device (RT-LAMP-LFD) assay to rapidly detect H7N9 virus was developed and evaluated. The RT-LAMP primers were designed to target the haemagglutinin (HA) and neuraminidase (NA) genes of H7N9 virus. Results of 10-fold dilution series assays showed that analysis of RT-LAMP products by the LFD method was as sensitive as real-time turbidity detection, and that the analytic sensitivities of the HA and NA RT-LAMP assays were both 10 copies of synthetic RNA. Furthermore, both the assays showed 100% clinical specificity for identification of H7N9 virus. The performance characteristics of the RT-LAMP-LFD assay were evaluated with 80 clinical specimens collected from suspected H7N9 patients. The NA RT-LAMP-LFD assay was more sensitive than real time RT-PCR assay. Compared with a combination of virus culture and real-time RT-PCR, the sensitivity, specificity, positive predictive value, and negative predictive value of the RT-LAMP-LFD assay were all 100%. Overall, The RT-LAMP-LFD assay established in this study can be used as a reliable method for early diagnosis of the avian-origin influenza A (H7N9) virus infection.
has issue date	2013-08-01(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0069941
bibo:pmid	23936359
has license	cc-by
sha1sum (hex)	f81ae57f3c989bf53683a523da0a1799ebd19862
schema:url	https://doi.org/10.1371/journal.pone.0069941
resource representing a document's title	Rapid and Sensitive Detection of Novel Avian-Origin Influenza A (H7N9) Virus by Reverse Transcription Loop-Mediated Isothermal Amplification Combined with a Lateral-Flow Device
has PubMed Central identifier	PMC3731295
has PubMed identifier	23936359
schema:publication	PLoS One
resource representing a document's body	covid:f81ae57f3c989bf53683a523da0a1799ebd19862#body_text
is schema:about of	named entity 'real-time' named entity 'H7N9' named entity 'The' named entity 'haemagglutinin' named entity 'The' named entity 'assay' named entity 'H7N9' named entity 'Detection' named entity 'Novel' named entity 'Isothermal' named entity 'COLLECTED' named entity 'HUMANS' named entity 'CASES' named entity 'METHOD' named entity 'RNA' named entity '100%' named entity 'RELIABLE' named entity 'LAMP' named entity 'HAEMAGGLUTININ' named entity 'SPECIFICITY' named entity 'AVIAN' named entity 'PRIMERS' named entity 'SYNTHETIC' named entity 'AMPLIFICATION' named entity 'OVERALL' named entity 'INFLUENZA A' named entity 'STUDY' named entity 'H7N9' named entity 'REVERSE TRANSCRIPTION' named entity 'FOLD DILUTION' named entity 'VIRUS' named entity 'RESULTS' named entity 'SENSITIVE' named entity 'LFD' named entity 'POSITIVE PREDICTIVE VALUE' named entity 'TURBIDITY' named entity 'RAPIDLY' named entity 'COMBINATION' named entity 'NEEDED' named entity 'VIRUS INFECTION' named entity 'INFECTION CONTROL' named entity 'USED' named entity 'EPIDEMIOLOGIC' named entity 'DIFFERENTIATE' named entity 'PATIENTS' named entity 'SEVERE DISEASE' named entity 'NOVEL' named entity 'LATERAL' named entity 'DEVICE' named entity 'EARLY DIAGNOSIS' named entity 'PERFORMANCE' named entity 'CAUSED BY' named entity 'REAL-TIME RT-PCR' named entity 'EVALUATED' named entity 'RT-PCR ASSAY' named entity 'CHARACTERISTICS' named entity 'COMPARED' named entity 'SENSITIVITY' named entity 'DISEASE' named entity 'DETECTION' named entity 'IDENTIFICATION' named entity 'DEATHS' named entity 'VIRUS' named entity 'ORIGIN' named entity 'COMBINED' named entity 'AVIAN' named entity 'NOVEL' named entity 'FLOW' named entity 'DEVICE' named entity 'ESTABLISHED' named entity 'RECENTLY' named entity 'DETECTION'

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