About: Molecular detection of novel picornaviruses in chickens and turkeys

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About: Molecular detection of novel picornaviruses in chickens and turkeys Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular detection of novel picornaviruses in chickens and turkeys
Creator	Saif, Yehia Ladman, Brian Parcells, Mark Farkas, Tibor Fey, Brittney Hargitt, • Murgia, Maria
Source	Medline; PMC
abstract	Fecal specimens, including swabs and litter extracts, collected from chickens, domestic ducks, turkeys, and Canadian geese were tested using degenerate primers targeting regions encoding for conserved amino acid motifs (YGDD and DY(T/S)(R/K/G)WDST) in calicivirus RNA-dependent RNA polymerases. Similar motifs are also present in other RNA viruses. Two fecal specimens and 18 litter extracts collected from chickens and turkeys yielded RT-PCR products. BLAST search and phylogenetic analysis revealed that all amplicons represented picornaviruses that clustered into two major groups. Four chicken and one turkey samples yielded 250 bp amplicons with 84–91% nucleotide identity to the recently described turkey hepatitis viruses, while 280 and 283 bp amplicons obtained from 11 chicken and 4 turkey samples represented novel picornaviruses with the closest nucleotide identity to kobuviruses (54–61%) and turdiviruses (47–54%). Analysis of 2.2–3.2 kb extended genome sequences including the partial P2 (2C) and complete P3 (3A, 3B (VPg), 3C(pro), and 3D(pol)) regions of selected strains indicated that viruses yielding the 280/283 bp amplicons represent a putative new genus of Picornaviridae. The 3′-non-translated region (NTR) of the turkey hepatitis-like viruses described in this study was significantly longer (641–654 nt) than that of any of the other piconaviruses and included a putative short open reading frame (ORF). In summary, we report the molecular detection of novel picornaviruses that appear to be endemic in both chickens and turkeys.
has issue date	2011-12-13(xsd:dateTime)
bibo:doi	10.1007/s11262-011-0695-4
bibo:pmid	22160827
has license	no-cc
sha1sum (hex)	f9b3cd1e84c6b400ea238353b6319854e6a3275c
schema:url	https://doi.org/10.1007/s11262-011-0695-4
resource representing a document's title	Molecular detection of novel picornaviruses in chickens and turkeys
has PubMed Central identifier	PMC7089249
has PubMed identifier	22160827
schema:publication	Virus Genes
resource representing a document's body	covid:f9b3cd1e84c6b400ea238353b6319854e6a3275c#body_text
is schema:about of	named entity 'represented' named entity 'chickens' named entity 'amplicons' named entity 'RNA' named entity 'nucleotide' named entity 'turkeys' named entity 'encoding' named entity '654' named entity 'WDST' named entity 'amplicons' named entity 'chicken' named entity 'ducks' named entity 'conserved' named entity 'extracts' named entity 'degenerate primers' named entity 'motifs' named entity 'Turdidae' named entity 'RNA virus' named entity 'genus' named entity 'enteric viruses' named entity 'Senecavirus' named entity 'picornavirus' named entity 'evolutionary origin' named entity 'North Carolina' named entity 'samples' named entity 'open reading frame (ORF)' named entity 'hepatitis' named entity 'nucleotide' named entity 'nucleotide' named entity 'calicivirus' named entity 'picornaviruses' named entity 'viruses' named entity 'amplicons' named entity '2.2' named entity 'RNA viruses' named entity 'amplicons' named entity 'genus' named entity 'turkeys' named entity 'picornaviruses' named entity 'RSS' named entity 'virus' named entity 'origin of replication' named entity 'amino acid' named entity 'P290' named entity 'sequence analysis' named entity 'viruses' named entity 'DYS' named entity 'genus' named entity 'primers' named entity 'genus' named entity 'Neighbor-Joining' named entity 'viruses' named entity 'Parechovirus' named entity 'viruses' named entity 'genus' named entity 'RNA-dependent RNA polymerase' named entity 'ethidium bromide' named entity 'secondary structures' named entity 'UPGMA' named entity 'Genus' named entity 'RNA sequences' named entity 'picornavirus' named entity 'primers' named entity 'calicivirus' named entity 'pseudoknot' named entity 'RT-PCR' named entity 'amino acid' named entity 'Applied Biosystems' named entity 'cell culture' named entity 'genomes' named entity 'protein' named entity 'RSS' named entity 'Aichi' named entity 'kidney' named entity 'virus'

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