About: Conditional ligands for Asian HLA variants facilitate the definition of CD8(+) T-cell responses in acute and chronic viral diseases

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Conditional ligands for Asian HLA variants facilitate the definition of CD8(+) T-cell responses in acute and chronic viral diseases Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Conditional ligands for Asian HLA variants facilitate the definition of CD8(+) T-cell responses in acute and chronic viral diseases
Creator	Bertoletti, Antonio Chia, Adeline Tan, Yee-Joo Nadua, Karen Gehring, Adam Grotenbreg, Gijsbert Leong, Nam Tan, Anthony Oh, Hsueh-Ling Chang, Cynthia Froesig, Thomas Hadrup, Sine Lim, Pei Or, Ming Toh, Kai
source	Medline; PMC
abstract	Conditional ligands have enabled the high-throughput production of human leukocyte antigen (HLA) libraries that present defined peptides. Immunomonitoring platforms typically concentrate on restriction elements associated with European ancestry, and such tools are scarce for Asian HLA variants. We report 30 novel irradiation-sensitive ligands, specifically targeting South East Asian populations, which provide 93, 63, and 79% coverage for HLA-A, -B, and -C, respectively. Unique ligands for all 16 HLA types were constructed to provide the desired soluble HLA product in sufficient yield. Peptide exchange was accomplished for all variants as demonstrated by an ELISA-based MHC stability assay. HLA tetramers with redirected specificity could detect antigen-specific CD8(+) T-cell responses against human cytomegalovirus, hepatitis B (HBV), dengue virus (DENV), and Epstein-Barr virus (EBV) infections. The potential of this population-centric HLA library was demonstrated with the characterization of seven novel T-cell epitopes from severe acute respiratory syndrome coronavirus, HBV, and DENV. Posthoc analysis revealed that the majority of responses would be more readily identified by our unbiased discovery approach than through the application of state-of-the-art epitope prediction. This flow cytometry-based technology therefore holds considerable promise for monitoring clinically relevant antigen-specific T-cell responses in populations of distinct ethnicity.
has issue date	2012-12-26(xsd:dateTime)
bibo:doi	10.1002/eji.201243088
bibo:pmid	23280567
has license	cc-by
sha1sum (hex)	fac9ef6ba1ca4bdb16baad83f0146e6a5c3b906f
schema:url	https://doi.org/10.1002/eji.201243088
resource representing a document's title	Conditional ligands for Asian HLA variants facilitate the definition of CD8(+) T-cell responses in acute and chronic viral diseases
has PubMed Central identifier	PMC3655610
has PubMed identifier	23280567
schema:publication	Eur J Immunol
resource representing a document's body	covid:fac9ef6ba1ca4bdb16baad83f0146e6a5c3b906f#body_text
is schema:about of	named entity 'T-CELL' named entity 'RESPONSES' named entity 'mer' named entity 'CHRONIC' named entity 'ACUTE' named entity 'JOURNAL ' named entity 'VIRAL DISEASES' named entity 'PEPTIDES' named entity 'SUPPORTING INFORMATION' named entity 'OVERLAPPING' named entity 'PEPTIDE' named entity 'TOTAL' named entity 'VARIANTS' named entity 'DERIVED' named entity 'HLA' named entity 'CD8' named entity 'LIGANDS' named entity 'EUROPEAN' named entity 'TESTED FOR' named entity 'ASIAN' named entity 'IMMUNOLOGY' named entity 'CONDITIONAL' named entity 'SUPPORTING INFORMATION' named entity 'TO STABILIZE' named entity 'FIGURE' named entity 'THEIR' named entity 'DIFFERENT' named entity 'ANTIGEN' named entity 'DEFINITION' named entity 'CAPABILITY' named entity 'DESIGN' named entity 'MER' named entity 'HLA' named entity 'mer' named entity 'tested' named entity 'total' named entity 'T-cell' named entity 'T-cell' named entity 'CD8 +' named entity 'ligands' named entity 'solid phase peptide synthesis' named entity 'antigens' named entity 'HLA' named entity 'ligands' named entity 'PBMCs' named entity 'm/z' named entity 'CD8' named entity 'staining' named entity 'Culturing' named entity 'isomers' named entity 'FlowJo' named entity 'antigen' named entity 'peptides' named entity 'forward scatter' named entity 'forward scatter' named entity 'EBV' named entity 'mass to charge ratios' named entity 'negative staining' named entity 'serum' named entity 'C18' named entity 'tetramer' named entity 'HLA' named entity 'amino acid sequences' named entity 'isomers' named entity 'RPMI' named entity 'isomers' named entity 'antibodies'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software