About: Deduced sequence of the bovine coronavirus spike protein and identification of the internal proteolytic cleavage site

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About: Deduced sequence of the bovine coronavirus spike protein and identification of the internal proteolytic cleavage site Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Deduced sequence of the bovine coronavirus spike protein and identification of the internal proteolytic cleavage site
Creator	Lapps, William Abraham, Sushma Brian~, David Kienzle,', Thomas
Source	Elsevier; Medline; PMC
abstract	Abstract The sequence of the spike (also called peplomer or E2) protein gene of the Mebus strain of bovine coronavirus (BCV) was obtained from cDNA clones of genomic RNA. The gene sequence predicts a 150,825 mol wt apoprotein of 1363 amino acids having an N-terminal hydrophobic signal sequence of 17 amino acids, 19 potential N-linked glycosylation sites, a hydrophobic anchor sequence of approximately 17 amino acids near the C terminus, and a hydrophilic cysteinerich C terminus of 35 amino acids. An internal LysArgArgSerArgArg sequence predicts a protease cleavage site between amino acids 768 and 769 that would separate the S apoprotein into S1 and S2 segments of 85690 and 65153 mol wt, respectively. Amino terminal amino acid sequencing of the virion-derived gp100 spike subunit confirmed the location of the predicted cleavage site, and established that gp120 and gp100 are the glycosylated virion forms of the S1 and S2 subunits, respectively. Sequence comparisons between BCV and the antigenically related mouse hepatitis coronavirus revealed more sequence divergence in the putative knob region of the spike protein (S1) than in the stem region (S2).
has issue date	1990-05-31(xsd:dateTime)
bibo:doi	10.1016/0042-6822(90)90257-r
bibo:pmid	2184576
has license	els-covid
sha1sum (hex)	fad4f28e9ed4392900febb77e26ab54c5435a5fb
schema:url	https://doi.org/10.1016/0042-6822%2890%2990257-r
resource representing a document's title	Deduced sequence of the bovine coronavirus spike protein and identification of the internal proteolytic cleavage site
has PubMed Central identifier	PMC7157924
has PubMed identifier	2184576
schema:publication	Virology
resource representing a document's body	covid:fad4f28e9ed4392900febb77e26ab54c5435a5fb#body_text
is schema:about of	named entity 'subunit' named entity 'sequence' named entity 'Proteolytic' named entity 'PROTEIN ' named entity 'BOVINE CORONAVIRUS' named entity '769' named entity 'HYDROPHOBIC' named entity 'SITES' named entity 'SER-' named entity 'SEPARATE' named entity 'INTERNAL' named entity 'PROTEASE CLEAVAGE' named entity 'SEQUENCE' named entity 'STRAIN' named entity 'TERMINUS' named entity 'CLONES' named entity 'CDNA' named entity 'N-TERMINAL ' named entity 'OBTAINED' named entity 'RNA' named entity 'PREDICTED' named entity 'CLEAVAGE' named entity 'LOCATION' named entity 'AMINO ACIDS' named entity 'GLYCOSYLATED' named entity '768' named entity 'AMINO TERMINAL' named entity 'ANCHOR' named entity 'HYDROPHILIC' named entity 'LYS-ARG' named entity 'SPIKE' named entity '150' named entity 'SUBUNIT' named entity 'APPROXIMATELY' named entity 'PROTEOLYTIC' named entity 'SITE' named entity 'VIRION' named entity 'ARG-SER' named entity 'SIGNAL SEQUENCE' named entity 'FORMS' named entity 'PROTEIN GENE' named entity 'INTERNAL' named entity 'IDENTIFICATION' named entity 'CLEAVAGE' named entity 'MOL' named entity 'SEQUENCING' named entity 'N-LINKED GLYCOSYLATION' named entity 'SITE' named entity 'NEAR' named entity 'AMINO ACID' named entity 'APOPROTEIN' named entity 'ARG-ARG' named entity 'BOVINE CORONAVIRUS' named entity 'SEGMENTS' named entity 'DERIVED' named entity 'SEQUENCE' named entity 'SPIKE PROTEIN' named entity 'ESTABLISHED' named entity 'GENOMIC' named entity 'CONFIRMED' named entity 'POTENTIAL' named entity 'BCV' named entity 'hydrophobic' named entity 'protease' named entity 'sites' named entity 'mol' named entity 'cleavage' named entity 'gene' named entity 'C terminus' named entity 'amino acids' named entity 'cleavage' named entity 'Protein' named entity 'cleavage site' named entity 'glycosylated' named entity 'amino acid sequencing' named entity 'Arg' named entity 'cDNA clones' named entity 'hydrophobic'

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