About: SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract

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About: SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract
Creator	Chen, Gang Hou, Yixuan Baric, Ralph Gralinski, Lisa Schäfer, Alexandra Yount, Boyd Sun, Ling Jaspers, Ilona Dinnon, Kenneth Livraghi-Butrico, Alessandra Lee, T Cameron, Cheryl Kelvin, David Chen, T Cameron, Mark De Silva, Aravinda Hou, Y Kimple, Adam Leist, Sarah Martinez, Fernando Olivier, Kenneth Randell, Scott Sun, S Kato, K Boucher, Richard Ghio, Andrew Asakura, Takanori Borczuk, Alain Martinez, David Salvatore, Steven Tse, Longping Bartelt, Luther Edwards, Caitlin Margolis, David Markmann, Alena Tata, Purushothama Cameron, A Silva, De Asakura, D Borczuk, S Boucher, S Cameron, M Dang, A Dang, Hong Fulcher, L Fulcher, M Ghio, K Gilmore, H Gilmore, Rodney Gralinski, S Kato, Takafumi Kelvin, C Kimple, V Lee, Rhianna Markmann, D Martinez, C Martinez, R Mascenik, Teresa Nakano, R Nakano, Satoko Neal, O Nicely, Nathan O'neal, Wanda Okuda, K Okuda, Kenichi Olivier, G Randell, W Salvatore, F Sontake, P Sontake, Vishwaraj Tata, A Tse, A Zumwalt, Ross
Source	Elsevier; Medline; PMC
abstract	Summary The mode of acquisition and causes for the variable clinical spectrum of COVID-19 remain unknown. We utilized a reverse genetics system to generate a GFP reporter virus to explore SARS-CoV-2 pathogenesis and a luciferase reporter virus to demonstrate sera collected from SARS and COVID-19 patients exhibited limited cross-CoV neutralization. High-sensitivity RNA in situ mapping revealed the highest ACE2 expression in the nose with decreasing expression throughout the lower respiratory tract, paralleled by a striking gradient of SARS-CoV-2 infection in proximal (high) vs distal (low) pulmonary epithelial cultures. COVID-19 autopsied lung studies identified focal disease and, congruent with culture data, SARS-CoV-2-infected ciliated and type 2 pneumocyte cells in airway and alveolar regions, respectively. These findings highlight the nasal susceptibility to SARS-CoV-2 with likely subsequent aspiration-mediated virus seeding to the lung in SARS-CoV-2 pathogenesis. These reagents provide a foundation for investigations into virus-host interactions in protective immunity, host susceptibility, and virus pathogenesis.
has issue date	2020-05-27(xsd:dateTime)
bibo:doi	10.1016/j.cell.2020.05.042
bibo:pmid	32526206
has license	els-covid
sha1sum (hex)	fdc688e9ac336413d216a337b9129c7add615512
schema:url	https://doi.org/10.1016/j.cell.2020.05.042
resource representing a document's title	SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract
has PubMed Central identifier	PMC7250779
has PubMed identifier	32526206
schema:publication	Cell
resource representing a document's body	covid:fdc688e9ac336413d216a337b9129c7add615512#body_text
is schema:about of	named entity 'Journal' named entity 'virus' named entity 'limited' named entity 'virus' named entity 'COVID-19' named entity 'virus' named entity 'virus' named entity 'Infection' named entity 'viruses'

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