About: Solexa sequencing assay   Goto Sponge  NotDistinct  Permalink

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AttributesValues
type
label
  • Solexa sequencing assay
subClassOf
described by
term editor
  • Philippe Rocca-Serra
example of usage
  • PMID: 18987734
definition
  • A DNA sequencing by synthesis assay that identifies sequence information using DNA polymerase and reversible terminator, requiring immobilization of genomic DNA fragments onto a surface and a specific clonal amplification step known as bridge PCR. Reliance on a reversible terminator allows cycles of DNA chain extension by DNA polymerase and imaging without the need of electrophoretic separation of newly synthesized DNA fragment as with Sanger sequencing.
  • A DNA sequencing by synthesis assay that identifies sequence information using DNA polymerase and reversible terminator, requiring immobilization of genomic DNA fragments onto a surface and a specific clonal amplification step known as bridge PCR. Reliance on a reversible terminator allows cycles of DNA chain extension by DNA polymerase and imaging without the need of electrophoretic separation of newly synthesized DNA fragment as with Sanger sequencing.
definition source
  • Solexa web site (SS_DNAsequencing.pdf document available on july 2009)
alternative label
  • reversible terminator sequencing
editor preferred term
  • Solexa sequencing assay
has curation status
is topic of
is hasBody of
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