About: Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature
Creator	Zaki, Ali Abdallah, Nermeen Abdel-Salam, Shimaa
Source	Elsevier; PMC
abstract	Abstract Middle East Respiratory Syndrome Coronavirus (MERS-CoV), is an emerging virus causing a highly fatal respiratory disease in humans. Confirmation of MERS-CoV infection and molecular study on the virus may require transportation of samples to specialized laboratories. While freezing at -80°C is the gold standard method for RNA preservation, maintaining the integrity of viral RNA during transport will require additional precautions and, as a result, increase transport costs. We aimed at testing the stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature for 16 weeks. Respiratory samples spiked with stock culture of MERS-CoV were extracted and loaded on QIAamp Viral RNA Mini Kit spin columns and preserved at room temperature. Amount of Viral RNA was evaluated periodically by real-time quantitative RT-PCR. Minimal changes in cycle threshold values over the study period was noted, suggesting stability of viral RNA by this preservation method.
has issue date	2020-08-21(xsd:dateTime)
bibo:doi	10.1016/j.diagmicrobio.2020.115182
has license	els-covid
sha1sum (hex)	00c68f41fde9491aa14a5c12a98114d7c2d78e8a
schema:url	https://doi.org/10.1016/j.diagmicrobio.2020.115182
resource representing a document's title	Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature
has PubMed Central identifier	PMC7441011
schema:publication	Diagn Microbiol Infect Dis
resource representing a document's body	covid:00c68f41fde9491aa14a5c12a98114d7c2d78e8a#body_text
is schema:about of	named entity 'highly' named entity 'realtime' named entity 'columns' named entity 'EXTRACTED' named entity 'STUDY' covid:arg/00c68f41fde9491aa14a5c12a98114d7c2d78e8a named entity 'quantitative' named entity 'spin' named entity 'cycle' named entity 'humans' named entity 'RNA extraction' named entity 'Abstract' named entity 'manuscript' named entity 'RNA' named entity 'values' named entity 'RNA' named entity 'Zaki' named entity 'All' named entity 'respiratory disease' named entity 'maintaining' named entity 'result' named entity 'involved' named entity 'coronavirus' named entity 'Stability' named entity 'coronavirus' named entity 'MERS-CoV' named entity 'Middle East Respiratory Syndrome Coronavirus' named entity 'Saudi Arabia' named entity 'respiratory disease' named entity 'RNA' named entity 'MERS-CoV' named entity 'RNA' named entity 'respiratory disease' named entity 'AW1' named entity 'MERS-CoV' named entity 'extract RNA' named entity 'Applied Biosystems' named entity 'Germany' named entity 'viral RNA' named entity 'virus' named entity 'RNA' named entity 'biosafety' named entity 'Ain Shams University' named entity 'Mathay' named entity 'MERS-CoV' named entity 'virus' named entity 'viral RNA' named entity 'RNA' named entity 'virus' named entity 'reverse-transcription polymerase chain reaction' named entity 'real-time PCR' named entity 'sputum' named entity 'RNA' named entity 'Mathay' named entity 'RNA extraction' named entity 'RT-PCR' named entity 'RNA' named entity 'tissue processing' named entity 'MERS-CoV' named entity 'Egypt' named entity 'RNA' named entity 'qRT-PCR' named entity 'MERS-CoV' named entity 'nasopharyngeal aspirate' named entity 'Thermo Fisher Scientific' named entity 'lysed' named entity 'WHO' named entity 'RNA' named entity 'clinical samples' named entity 'California' named entity 'gene' named entity 'Infectious bronchitis virus' named entity 'phylogenetic analysis' named entity 'CDC' named entity 'dry ice' named entity 'titer' named entity 'RNA' named entity 'dry ice' named entity '21 days' named entity 'Middle East Respiratory Syndrome Coronavirus' named entity 'RNA extraction'

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