About: R3T (Rapid Research Response Team) One-step RT-qPCR kit for COVID-19 diagnostic using in-house enzymes

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: R3T (Rapid Research Response Team) One-step RT-qPCR kit for COVID-19 diagnostic using in-house enzymes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	R3T (Rapid Research Response Team) One-step RT-qPCR kit for COVID-19 diagnostic using in-house enzymes
Creator	Kusakabe, Takahiro Al-Qahtani, Ahmed Hashem, Anwar Lee, Jae Pain, Arnab Li, Mo Alhamlan, Fatimah Almontashiri, Naif Alofi, Fadwa Alsomali, Afrah Hala, Sharif Hamdan, Samir Khogeer, Asim Mon, Hiroaki Salunke, Rahul Tehseen, Muhammad Mfarrej, Sara Mandujano, Gerardo Sakashita, Kosuke Sobhy, Mohamed Takahashi, Etsuko Takahashi, Masateru
Source	MedRxiv
abstract	One-step RT-qPCR is the most widely applied method for COVID-19 diagnostics. Designing in-house one-step RT-qPCR kits is restricted by the patent-rights for the production of enzymes and the lack of information about the components of the commercial kits. Here, we provide a simple, economical, and powerful one-step RT-qPCR kit based on patent-free, specifically-tailored versions of Moloney Murine Leukemia Virus Reverse Transcriptase and Thermus aquaticus DNA polymerase termed the R3T (Rapid Research Response Team) One-step RT-qPCR. Our kit was routinely able to reliably detect as low as 10 copies of the synthetic RNAs of the SARS-CoV-2. More importantly, our kit successfully detected COVID-19 in clinical samples of broad viral titers with similar reliability and selectivity as that of the Invitrogen SuperScript III Platinum One-step RT-qPCR and TaqPath 1-Step RT-qPCR kits. Overall, our kit has shown robust performance in both of laboratory settings and the Saudi Ministry of Health-approved testing facility.
has issue date	2020-08-04(xsd:dateTime)
bibo:doi	10.1101/2020.07.31.20165704
has license	medrxiv
sha1sum (hex)	0116d59e5be0d8e7b23d96fdadfd0088261120c9
schema:url	https://doi.org/10.1101/2020.07.31.20165704
resource representing a document's title	R3T (Rapid Research Response Team) One-step RT-qPCR kit for COVID-19 diagnostic using in-house enzymes
resource representing a document's body	covid:0116d59e5be0d8e7b23d96fdadfd0088261120c9#body_text
is schema:about of	named entity 'One-step' named entity 'preprint' named entity 'respiratory illness' named entity 'RT-qPCR' named entity 'CDC' named entity 'hot-start' named entity 'CDC' named entity 'testing facilities' named entity 'RT-qPCR' named entity 'coronaviruses' named entity 'peer review' named entity 'tested positive' named entity 'preprint' named entity 'CoV' named entity 'Wuhan' named entity 'fever' named entity 'peer review' named entity 'preprint' named entity 'TaqMan' named entity 'preprint' named entity 'preprint' named entity 'RT-qPCR' named entity 'RT-qPCR' named entity 'cDNA' named entity 'Integrated DNA Technologies' named entity 'N-terminus' named entity 'preprint' named entity 'RT-qPCR' named entity 'preprint' named entity '1, 2' named entity 'severe acute respiratory syndrome' named entity 'medRxiv' named entity 'SARS-CoV-2' named entity 'preprint' named entity 'Saudi Arabia' named entity 'King Abdullah' named entity 'preprint' named entity 'RNAs' named entity 'RT-qPCR' named entity 'SARS-CoV-2' named entity 'CDC' named entity 'peer review' named entity 'novel-coronavirus' named entity 'RT-qPCR' named entity 'viral infection' named entity 'peer review' named entity 'COVID' named entity 'peer review' named entity 'preprint' named entity 'primer-dimer' named entity 'gene' named entity 'RNA' named entity 'preprint' named entity 'mixing ratio' named entity 'MMLV' named entity 'diagnostic tests' named entity 'true positive' named entity 'PCR' named entity 'RT-qPCR' named entity 'qPCR' named entity 'CDC' named entity 'vaccines' named entity 'gene' named entity 'RNAs'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software